Construct: ORF TRCN0000479199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008884.1_s317c1
- Derived from:
- ccsbBroadEn_11571
- DNA Barcode:
- TCATGCGACGACAGCGGAGAATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EHMT2 (10919)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10919 | EHMT2 | euchromatic histone lysine ... | NM_001318833.1 | 99.2% | 99.3% | 84A>G;1822_1842del |
| 2 | human | 10919 | EHMT2 | euchromatic histone lysine ... | NM_006709.5 | 82.6% | 82.7% | 1_627del;711A>G |
| 3 | human | 10919 | EHMT2 | euchromatic histone lysine ... | XM_006714976.1 | 82.2% | 82.2% | 1_627del;711A>G;2449_2469del |
| 4 | human | 10919 | EHMT2 | euchromatic histone lysine ... | NM_025256.7 | 79.8% | 79.9% | 1_627del;711A>G;1113_1114ins102 |
| 5 | human | 10919 | EHMT2 | euchromatic histone lysine ... | XM_017010202.1 | 79.4% | 79.4% | (many diffs) |
| 6 | human | 10919 | EHMT2 | euchromatic histone lysine ... | NM_001363689.1 | 78.9% | 79% | 1_798del;882A>G |
| 7 | human | 10919 | EHMT2 | euchromatic histone lysine ... | XM_006714974.1 | 78.5% | 78.5% | 1_798del;882A>G;2620_2640del |
| 8 | human | 10919 | EHMT2 | euchromatic histone lysine ... | NM_001289413.1 | 76.2% | 76.3% | 1_798del;882A>G;1284_1285ins102 |
| 9 | human | 10919 | EHMT2 | euchromatic histone lysine ... | XM_006714975.1 | 75.8% | 75.9% | (many diffs) |
| 10 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | NM_001286575.1 | 73.1% | 80% | (many diffs) |
| 11 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | XM_006523482.3 | 72.5% | 79.3% | (many diffs) |
| 12 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | NM_147151.2 | 70.3% | 77.2% | (many diffs) |
| 13 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | NM_145830.2 | 69.8% | 76.4% | (many diffs) |
| 14 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | XM_011246248.1 | 69.2% | 75.8% | (many diffs) |
| 15 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | NM_001286573.1 | 67.2% | 73.7% | (many diffs) |
| 16 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | XM_011246249.1 | 66.6% | 73.1% | (many diffs) |
| 17 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | XR_001782028.1 | 63% | (many diffs) | |
| 18 | mouse | 110147 | Ehmt2 | euchromatic histone lysine ... | XR_876459.1 | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3069
- ORF length:
- 3003
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgatgatgtc cactcactgg gaaaggtgac ctcagatctg gccaaaagga 121 ggaagctgaa ctcaggaggt ggcctgtcgg aggagttagg ttctgcccgg cgttcaggag 181 aagtgaccct gacgaaaggg gaccccgggt ccctggagga gtgggagacg gtggtgggtg 241 atgacttcag tctctactat gattcctact ctgtggatga gcgcgtggac tccgacagca 301 agtctgaagt tgaagctcta actgaacaac taagtgaaga ggaggaggag gaagaggagg 361 aagaagaaga agaggaagag gaggaggaag aggaagaaga agaggaagat gaggagtcag 421 ggaatcagtc agataggagt ggttccagtg gccggcgcaa ggccaagaag aaatggcgaa 481 aagacagccc atgggtgaag ccgtctcgga aacggcgcaa gcgggagcct ccgcgggcca 541 aggagccacg aggagtgaat ggtgtgggct cctcaggccc cagtgagtac atggaggtcc 601 ctctggggtc cctggagctg cccagcgagg ggaccctctc ccccaaccac gctggggtgt 661 ccaatgacac atcttcgctg gagacagagc gagggtttga ggagttgccc ctgtgcagct 721 gccgcatgga ggcacccaag attgaccgca tcagcgagag ggcggggcac aagtgcatgg 781 ccactgagag tgtggacgga gagctgtcag gctgcaatgc cgccatcctc aagcgggaga 841 ccatgaggcc atccagccgt gtggccctga tggtgctctg tgagacccac cgcgcccgca 901 tggtcaaaca ccactgctgc ccgggctgcg gctacttctg cacggcgggc accttcctgg 961 agtgccaccc tgacttccgt gtggcccacc gcttccacaa ggcctgtgtg tctcagctga 1021 atgggatggt cttctgtccc cactgtgggg aggatgcttc tgaagctcaa gaggtgacca 1081 tcccccgggg tgacggggtg accccaccgg ccggcactgc agctcctgca cccccacccc 1141 tgtcccagga tgtccccggg agagcagaca cttctcagcc cagtgcccgg atgcgagggc 1201 atggggaacc ccggcgcccg ccctgcgatc ccctggctga caccattgac agctcagggc 1261 cctccctgac cctgcccaat gggggctgcc tttcagccgt ggggctgcca ctggggccag 1321 gccgggaggc cctggaaaag gccctggtca tccaggagtc agagaggcgg aagaagctcc 1381 gtttccaccc tcggcagttg tacctgtccg tgaagcaggg cgagctgcag aaggtgatcc 1441 tgatgctgtt ggacaacctg gaccccaact tccagagcga ccagcagagc aagcgcacgc 1501 ccctgcatgc agccgcccag aagggctccg tggagatctg ccatgtgctg ctgcaggctg 1561 gagccaacat aaatgcagtg gacaaacagc agcggacgcc actgatggag gccgtggtga 1621 acaaccacct ggaggtagcc cgttacatgg tgcagcgtgg tggctgtgtc tatagcaagg 1681 aggaggacgg ttccacctgc ctccaccacg cagccaaaat cgggaacttg gagatggtca 1741 gcctgctgct gagcacagga caggtggacg tcaacgccca ggacagtggg gggtggacgc 1801 ccatcatctg ggctgcagag cacaagcaca tcgaggtgat ccgcatgcta ctgacgcggg 1861 gcgccgacgt caccctcact gacaacgagg agaacatctg cctgcactgg gcctccttca 1921 cgggcagcgc cgccatcgcc gaagtccttc tgaatgcgcg ctgtgacctc catgctgtca 1981 actaccatgg ggacaccccc ctgcacatcg cagctcggga gagctaccat gactgcgtgc 2041 tgttattcct gtcacgtggg gccaaccctg agctgcggaa caaagagggg gacacagcat 2101 gggacctgac tcccgagcgc tccgacgtgt ggtttgcgct tcaactcaac cgcaagctcc 2161 gacttggggt gggaaatcgg gccatccgca cagagaagat catctgccgg gacgtggctc 2221 ggggctatga gaacgtgccc attccctgtg tcaacggtgt ggatggggag ccctgccctg 2281 aggattacaa gtacatctca gagaactgcg agacgtccac catgaacatc gatcgcaaca 2341 tcacccacct gcagcactgc acgtgtgtgg acgactgctc tagctccaac tgcctgtgcg 2401 gccagctcag catccggtgc tggtatgaca aggatgggcg attgctccag gaatttaaca 2461 agattgagcc tccgctgatt ttcgagtgta accaggcgtg ctcatgctgg agaaactgca 2521 agaaccgggt cgtacagagt ggcatcaagg tgcggctaca gctctaccga acagccaaga 2581 tgggctgggg ggtccgcgcc ctgcagacca tcccacaggg gaccttcatc tgcgagtatg 2641 tcggggagct gatctctgat gctgaggctg atgtgagaga ggatgattct tacctcttcG 2701 ACTTAGACAA CAAGGATGGA GAGGTGTACT GCATAGATGC CCGTTACTAT GGCAACATCA 2761 GCCGCTTCAT CAACCACCTG TGTGACCCCA ACATCATTCC CGTCCGGGTC TTCATGCTGC 2821 ACCAAGACCT GCGATTTCCA CGCATCGCCT TCTTCAGTTC CCGAGACATC CGGACTGGGG 2881 AGGAGCTAGG GTTTGACTAT GGCGACCGCT TCTGGGACAT CAAAAGCAAA TATTTCACCT 2941 GCCAATGTGG CTCTGAGAAG TGCAAGCACT CAGCCGAAGC CATTGCCCTG GAGCAGAGCC 3001 GTCTGGCCCG CCTGGACCCA CACCCTGAGC TGCTGCCCGA GCTCGGCTCC CTGCCCCCTG 3061 TCAACACATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3121 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTTTTCGAT 3181 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCATGCG ACGACAGCGG AGAATAGACG 3241 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt