Transcript: Mouse XR_876459.1

PREDICTED: Mus musculus euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ehmt2 (110147)
Length:
3952
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_876459.1
NBCI Gene record:
Ehmt2 (110147)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_876459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000310845 GCAACAACGCACGCCACTAAT pLKO_005 2352 3UTR 100% 13.200 18.480 N Ehmt2 n/a
2 TRCN0000054546 CCTCTTCGATTTAGATAACAA pLKO.1 3343 3UTR 100% 5.625 7.875 N Ehmt2 n/a
3 TRCN0000301155 CCTCTTCGATTTAGATAACAA pLKO_005 3343 3UTR 100% 5.625 7.875 N Ehmt2 n/a
4 TRCN0000054547 CGAAGCTCTAGCTGAACAGTT pLKO.1 1044 3UTR 100% 4.950 6.930 N Ehmt2 n/a
5 TRCN0000301233 CGAAGCTCTAGCTGAACAGTT pLKO_005 1044 3UTR 100% 4.950 6.930 N Ehmt2 n/a
6 TRCN0000054544 GCCAACATCAATGCCGTAGAT pLKO.1 2329 3UTR 100% 4.950 3.465 N Ehmt2 n/a
7 TRCN0000304242 AGACATTTCTCCATCAGAGAC pLKO_005 3756 3UTR 100% 4.050 2.835 N Ehmt2 n/a
8 TRCN0000054545 CCGAGAGAGTTCATAGCTCTT pLKO.1 287 3UTR 100% 4.050 2.835 N Ehmt2 n/a
9 TRCN0000174241 CCGAGAGAGTTCATAGCTCTT pLKO.1 287 3UTR 100% 4.050 2.835 N Ehmt2 n/a
10 TRCN0000301235 CCGAGAGAGTTCATAGCTCTT pLKO_005 287 3UTR 100% 4.050 2.835 N Ehmt2 n/a
11 TRCN0000054543 GCCAAGAAGAAATGGCGGAAA pLKO.1 1198 3UTR 100% 4.050 2.835 N Ehmt2 n/a
12 TRCN0000190517 GAGGAGGAAGAAGAAGAAGAA pLKO.1 1087 3UTR 100% 4.950 2.475 Y G430095P16Rik n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_876459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11571 pDONR223 100% 62.5% None (many diffs) n/a
2 ccsbBroad304_11571 pLX_304 0% 62.5% V5 (many diffs) n/a
3 TRCN0000479199 TCATGCGACGACAGCGGAGAATAG pLX_317 12.5% 62.5% V5 (many diffs) n/a
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