Construct: ORF TRCN0000479206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008189.1_s317c1
- Derived from:
- ccsbBroadEn_05564
- DNA Barcode:
- CGGAATGTGATGCCCCCCCGGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPSR1 (387129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387129 | NPSR1 | neuropeptide S receptor 1 | NM_207173.2 | 100% | 100% | |
| 2 | human | 387129 | NPSR1 | neuropeptide S receptor 1 | NM_001300933.2 | 97% | 97% | 276_277ins33 |
| 3 | human | 387129 | NPSR1 | neuropeptide S receptor 1 | NM_207172.2 | 92.8% | 91.5% | (many diffs) |
| 4 | human | 387129 | NPSR1 | neuropeptide S receptor 1 | NM_001300935.1 | 91.4% | 87.5% | (many diffs) |
| 5 | human | 387129 | NPSR1 | neuropeptide S receptor 1 | NM_001300934.1 | 75.7% | 74% | (many diffs) |
| 6 | mouse | 319239 | Npsr1 | neuropeptide S receptor 1 | NM_175678.3 | 82.1% | 81.6% | (many diffs) |
| 7 | mouse | 319239 | Npsr1 | neuropeptide S receptor 1 | XM_017313417.1 | 82.1% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1200
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccagccaac ttcacagagg gcagcttcga ttccagtggg accgggcaga 121 cgctggattc ttccccagtg gcttgcactg aaacagtgac ttttactgaa gtggtggaag 181 gaaaggaatg gggttccttc tactactcct ttaagactga gcaattgata actctgtggg 241 tcctctttgt ttttaccatt gttggaaact ccgttgtgct tttttccaca tggaggagaa 301 agaagaagtc aagaatgacc ttctttgtga ctcagctggc catcacagat tctttcacag 361 gactggtcaa catcttgaca gatattaatt ggcgattcac tggagacttc acggcacctg 421 acctggtttg ccgagtggtc cgctatttgc aggttgtgct gctctacgcc tctacctacg 481 tcctggtgtc cctcagcata gacagatacc atgccatcgt ctaccccatg aagttccttc 541 aaggagaaaa gcaagccagg gtcctcattg tgatcgcctg gagcctgtct tttctgttct 601 ccattcccac cctgatcata tttgggaaga ggacactgtc caacggtgaa gtgcagtgct 661 gggccctgtg gcctgacgac tcctactgga ccccatacat gaccatcgtg gccttcctgg 721 tgtacttcat ccctctgaca atcatcagca tcatgtatgg cattgtgatc cgaactattt 781 ggattaaaag caaaaccTAC GAAACAGTGA TTTCCAACTG CTCAGATGGG AAACTGTGCA 841 GCAGCTATAA CCGAGGACTC ATCTCAAAGG CAAAAATCAA GGCTATCAAG TATAGCATCA 901 TCATCATTCT TGCCTTCATC TGCTGTTGGA GTCCATACTT CCTGTTTGAC ATTTTGGACA 961 ATTTCAACCT CCTTCCAGAC ACCCAGGAGC GTTTCTATGC CTCTGTGATC ATTCAGAACC 1021 TGCCAGCATT GAATAGTGCC ATCAACCCCC TCATCTACTG TGTCTTCAGC AGCTCCATCT 1081 CTTTCCCCTG CAGGGTCATC CGTCTCCGTC AGCTCCAGGA GGCTGCGCTA ATGCTCTGCC 1141 CTCAACGAGA GAACTGGAAG GGTACTTGGC CAGGTGTACC TTCCTGGGCT CTTCCAAGGT 1201 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGACGGAA TGTGATGCCC CCCCGGCCGA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt