Construct: ORF TRCN0000479256

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009668.2_s317c1
Derived from:
ccsbBroadEn_08233
DNA Barcode:
AAAAACGTTCGGCATAACTCACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SDF4 (51150)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479256
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51150 SDF4 stromal cell derived factor 4 NM_016176.4 99.9% 100% 570T>C
2 human 51150 SDF4 stromal cell derived factor 4 NM_016547.2 88.9% 82.8% (many diffs)
3 human 51150 SDF4 stromal cell derived factor 4 XM_024447473.1 88.8% 88.9% 570T>C;736_870del
4 human 51150 SDF4 stromal cell derived factor 4 XM_011541556.1 55% 53.8% (many diffs)
5 mouse 20318 Sdf4 stromal cell derived factor 4 NM_001302467.1 82.8% 87% (many diffs)
6 mouse 20318 Sdf4 stromal cell derived factor 4 NM_001302468.1 82.8% 87% (many diffs)
7 mouse 20318 Sdf4 stromal cell derived factor 4 NM_011341.5 82.8% 87% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1152
ORF length:
1086
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ctggccctgg gtggcgatgg cgtccaggtg gggtcccctc attggcctgg
121ctccgtgctg cctctggctc ctgggggcag tccttctgat ggacgcgtct gcacggcctg
181ccaaccactc gtccactcga gagagagtag ccaacaggga ggagaatgag atcctgcccc
241cagaccacct gaacggggtg aagctggaga tggacgggca cctcaatcgc ggcttccacc
301aggaggtctt cctaggcaag gacctgggtg gctttgatga ggacgcggag ccgcggcgga
361gccggaggaa gctgatggtc atcttttcca aggtggatgt gaacactgac cggaagatca
421gtgccaagga gatgcagcgc tggatcatgg agaagacggc cgagcacttc caggaggcca
481tggaggagag caagacacac ttccgcgccg tggaccctga cggggacggt cacgtgtctt
541gggacgagta taaggtgaag tttttggcga gtaaaggcca tagcgagaag gaggttgccg
601acgccatcag gctcaacgag gaactcaaag tggacgagga aacacaggaa gtcctggaga
661acctgaagga ccgctggtac caggcggaca gcccccctgc agacctgctg ctgacggagg
721aggagttcct gtcgttcctc caccccgagc acagccgggg aatgctcagg ttcatggtga
781aggagatcgt ccgggacctg GACCAGGACG GTGACAAGCA GCTCTCTGTG CCCGAGTTCA
841TCTCCCTGCC CGTGGGCACC GTGGAGAACC AGCAGGGCCA GGACATTGAC GACAACTGGG
901TGAAAGACAG AAAAAAGGAG TTTGAGGAGC TCATTGACTC CAACCACGAC GGCATCGTGA
961CCGCCGAGGA GCTGGAGAGC TACATGGACC CCATGAACGA GTACAACGCG CTGAACGAGG
1021CCAAGCAGAT GATCGCCGTC GCCGACGAGA ACCAGAACCA CCACCTGGAG CCCGAGGAGG
1081TGCTCAAGTA CAGCGAGTTC TTCACGGGCA GCAAGCTGGT GGACTACGCG CGCAGCGTGC
1141ACGAGGAGTT TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1201ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1261CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA AACGTTCGGC ATAACTCACA
1321CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)