Construct: ORF TRCN0000479279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000374.1_s317c1
- Derived from:
- ccsbBroadEn_13991
- DNA Barcode:
- CCGATTGTTCTGTATCCCATCTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NCOA3 (8202)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479279
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8202 | NCOA3 | nuclear receptor coactivator 3 | NM_006534.4 | 99.9% | 85.4% | 3638A>C;3640delC |
| 2 | human | 8202 | NCOA3 | nuclear receptor coactivator 3 | NM_001174087.2 | 99.7% | 85.3% | 3637_3646delCAGGTGAGCT;3649C>G |
| 3 | human | 8202 | NCOA3 | nuclear receptor coactivator 3 | NM_181659.3 | 99.6% | 85.2% | 3638A>C;3640_3652del |
| 4 | human | 8202 | NCOA3 | nuclear receptor coactivator 3 | NM_001174088.2 | 98.2% | 83.8% | (many diffs) |
| 5 | mouse | 17979 | Ncoa3 | nuclear receptor coactivator 3 | NM_008679.3 | 80.3% | 72.9% | (many diffs) |
| 6 | mouse | 17979 | Ncoa3 | nuclear receptor coactivator 3 | XM_006498888.2 | 80.3% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3756
- ORF length:
- 3687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtggatta ggagaaaact tggatccact ggccagtgat tcacgaaaac 121 gcaaattgcc atgtgatact ccaggacaag gtcttacctg cagtggtgaa aaacggagac 181 gggagcagga aagtaaatat attgaagaat tggctgagct gatatctgcc aatcttagtg 241 atattgacaa tttcaatgtc aaaccagata aatgtgcgat tttaaaggaa acagtaagac 301 agatacgtca aataaaagag caaggaaaaa ctatttccaa tgatgatgat gttcaaaaag 361 ccgatgtatc ttctacaggg cagggagtta ttgataaaga ctccttagga ccgcttttac 421 ttcaggcatt ggatggtttc ctatttgtgg tgaatcgaga cggaaacatt gtatttgtat 481 cagaaaatgt cacacaatac ctgcaatata agcaagagga cctggttaac acaagtgttt 541 acaatatctt acatgaagaa gacagaaagg attttcttaa gaatttacca aaatctacag 601 ttaatggagt ttcctggaca aatgagaccc aaagacaaaa aagccataca tttaattgcc 661 gtatgttgat gaaaacacca catgatattc tggaagacat aaacgccagt cctgaaatgc 721 gccagagata tgaaacaatg cagtgctttg ccctgtctca gccacgagct atgatggagg 781 aaggggaaga tttgcaatct tgtatgatct gtgtggcacg ccgcattact acaggagaaa 841 gaacatttcc atcaaaccct gagagcttta ttaccagaca tgatctttca ggaaaggttg 901 tcaatataga tacaaattca ctgagatcct ccatgaggcc tggctttgaa gatataatcc 961 gaaggtgtat tcagagattt tttagtctaa atgatgggca gtcatggtcc cagaaacgtc 1021 actatcaaga agcttatctt aatggccatg cagaaacccc agtatatcga ttctcgttgg 1081 ctgatggaac tatagtgact gcacagacaa aaagcaaact cttccgaaat cctgtaacaa 1141 atgatcgaca tggctttgtc tcaacccact tccttcagag agaacagaat ggatatagac 1201 caaacccaaa tcctgttgga caagggatta gaccacctat ggctggatgc aacagttcgg 1261 taggcggcat gagtatgtcg ccaaaccaag gcttacagat gccgagcagc agggcctatg 1321 gcttggcaga ccctagcacc acagggcaga tgagtggagc taggtatggg ggttccagta 1381 acatagcttc attgacccct gggccaggca tgcaatcacc atcttcctac cagaacaaca 1441 actatgggct caacatgagt agccccccac atgggagtcc tggtcttgcc ccaaaccagc 1501 agaatatcat gatttctcct cgtaatcgtg ggagtccaaa gatagcctca catcagtttt 1561 ctcctgttgc aggtgtgcac tctcccatgg catcttctgg caatactggg aaccacagct 1621 tttccagcag ctctctcagt gccctgcaag ccatcagtga aggtgtgggg acttcccttt 1681 tatctactct gtcatcacca ggccccaaat tggataactc tcccaatatg aatattaccc 1741 aaccaagtaa agtaagcaat caggattcca agagtcctct gggcttttat tgcgaccaaa 1801 atccagtgga gagttcaatg tgtcagtcaa atagcagaga tcacctcagt gacaaagaaa 1861 gtaaggagag cagtgttgag ggggcagaga atcaaagggg tcctttggaa agcaaaggtc 1921 ataaaaaatt actgcagtta cttacctgtt cttctgatga ccggggtcat tcctccttga 1981 ccaactcccc cctagattca agttgtaaag aatcttctgt tagtgtcacc agcccctctg 2041 gagtctcctc ctctacatct ggaggagtat cctctacatc caatatgcat gggtcactgt 2101 tacaagagaa gcaccggatt ttgcacaagt tgctgcagaa tgggaattca ccagctgagg 2161 tagccaagat tactgcagaa gccactggga aagacaccag cagtataact tcttgtgggg 2221 acggaaatgt tgtcaagcag gagcagctaa gtcctaagaa gaaggagaat aatgcacttc 2281 ttagatacct gctggacagg gatgatccta gtgatgcact ctctaaagaa ctacagcccc 2341 aagtggaagg agtggataat aaaatgagtc agtgcaccag ctccaccatt cctagctcaa 2401 gtcaagagaa agaccctaaa attaagacag agacaagtga agagggatct ggagacttgg 2461 ataatctaga tgctattctt ggtgatctga ctagttctga cttttacaat aattccatat 2521 cctcaaatgg tagtcatctg gggactaagc aacaggtgtt tcaaggaact aattctctgg 2581 gtttgaaaag ttcacagtct gtgcagtcta ttcgtcctcc atataaccga gcagtgtctc 2641 tggatagccc tgtttctgtt ggctcaagtc ctccagtaaa aaatatcagt gctttcccca 2701 tgttaccaaa gcaacccatg ttgggtggga atccaagaat gatggatagt caggaaaatt 2761 atggctcaag tatgggtggg ccaaaccgaa atgtgactgt gactcagact ccttcctcag 2821 gagactgggg cttaccaaac tcaaaggccg gcagaatgga acctatgaat tcaaactcca 2881 tgggaagacc aggaggagat tataatactt ctttacccag acctgcactg ggtggctcta 2941 ttcccacatt gcctcttcgg tctaatagca taccaggtgc gagaccagta ttgcaacagc 3001 agcagcagat gcttcaaatg aggcctggtg aaatccccat gggaatgggg gctaatccct 3061 atggccaagc agcagcatct aaccaactgg gttcctggcc cgatggcatg ttgtccatgg 3121 aacaagtttc tcatggcact caaaataggc ctcttcttag gaattccctg gatgatcttg 3181 ttgggccacc ttccaacctg gaaggccaga gtgacgaaag agcattattg gaccagctgc 3241 acactcttct cagcaacaca gatgccacag gcctggaaga aattgacaga gctttgggca 3301 ttcctgaact tgtcaatcag ggacaggcat tagagcccaa acaggatgct ttccaaggcc 3361 aagaagcagc agtaatgatg gatcagaagg caggattata tggacagaca tacccagcac 3421 aggggcctcc aatgcaagga ggctttcatc ttcagggaca atcaccatct tttaactcta 3481 tgatgaatca gatgaaccag caaggcaatt ttcctctcca aggaatgcac ccacgagcca 3541 acatcatgag accccggaca aacaccccca agcaacttag aatgcagctt cagcagaggc 3601 tgcagggcca gcagtttttg aatcagagcc gacaggcact tgaattgaaa atggaaaacc 3661 ctactgctgg tggtgctgcg gtgatgaggc ctatgatgca gcccccgagg gttttcttaa 3721 tgctcaaatg gtcgcccaac gcagcagaga gctgctaagt catcacttcc gacaacagag 3781 ggtggctatg atgatgcagc agcagcagca gcagcaacag cagcagcagc agcagcagca 3841 gcagcaacag caacagcaac agcaacagca gcaacagcag caaacccagg ccttcagccc 3901 acctcctaat gtgactgctt cccccagcat ggatgggctt ttggcaggac ccacaatgcc 3961 acaagctccT CCGCAACAGT TTCCATATCA ACCAAATTAT GGAATGGGAC AACAACCAGA 4021 TCCAGCCTTT GGTCGAGTGT CTAGTCCTCC CAATGCAATG ATGTCGTCAA GAATGGGTCC 4081 CTCCCAGAAT CCCATGATGC AACACCCGCA GGCTGCATCC ATCTATCAGT CCTCAGAAAT 4141 GAAGGGCTGG CCATCAGGAA ATTTGGCCAG GAACAGCTCC TTTTCCCAGC AGCAGTTTGC 4201 CCACCAGGGG AATCCTGCAG TGTATAGTAT GGTGCACATG AATGGCAGCA GTGGTCACAT 4261 GGGACAGATG AACATGAACC CCATGCCCAT GTCTGGCATG CCTATGGGTC CTGATCAGAA 4321 ATACTGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 4381 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAG TATTTCGATT 4441 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCGATTGT TCTGTATCCC ATCTAAACGC 4501 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt