Construct: ORF TRCN0000479289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016665.1_s317c1
- Derived from:
- ccsbBroadEn_08821
- DNA Barcode:
- AGATCCGGCGTTCGCGACTTCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLSPN (63967)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479289
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63967 | CLSPN | claspin | NM_001190481.2 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 63967 | CLSPN | claspin | NM_022111.4 | 95% | 94.9% | (many diffs) |
| 3 | human | 63967 | CLSPN | claspin | XM_011541938.2 | 94.7% | 94.6% | (many diffs) |
| 4 | human | 63967 | CLSPN | claspin | XM_011541940.3 | 93.1% | 92.3% | (many diffs) |
| 5 | human | 63967 | CLSPN | claspin | NM_001330490.2 | 93% | 92.2% | (many diffs) |
| 6 | human | 63967 | CLSPN | claspin | XM_011541941.3 | 91% | 90.8% | (many diffs) |
| 7 | human | 63967 | CLSPN | claspin | XR_001737361.2 | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3894
- ORF length:
- 3825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaggcgag gtgggttctg aggttcacct agaaatcaat gacccaaacg 121 tcatttcaca agaggaagca gatagtcctt cagatagtgg acagggcggc tatgaaacaa 181 ttggaccctt gagtgaagga gattcagatg aagagatatt tgtaagtaag aagttgaaaa 241 acaggaaggt tctacaagac agtgattccg aaacagagga cacaaatgcc tctccagaga 301 aaactaccta tgacagtgcc gaggaggaaa ataaagagaa tttatatgct gggaaaaata 361 caaaaatcaa aaggatttac aaaactgtgg cagacagtga tgaaagttac atggaaaagt 421 ctttgtatca ggaaaatctt gaagcgcaag tgaaaccttg cttagagctg agtcttcagt 481 ctggaaactc tacagacttt accactgaca gaaagagttc caaaaagcac atacatgata 541 aagaaggaac tgcaggaaaa gcaaaagtaa aatcaaaaag aagacttgag aaagaggaga 601 gaaaaatgga aaaaattaga cagctaaaaa agaaggaaac aaaaaaccag gaagatgatg 661 tagaacagcc atttaatgac agtggctgtc ttcttgtgga taaagacctt tttgaaactg 721 ggttggagga tgaaaataac tctccattgg aagatgaaga gtcattagaa tcaataagag 781 cagctgtaaa aaacaaagta aaaaagcaca agaaaaaaga accatctttg gagagtgggg 841 tccattcatt tgaggaagga agtgagttat caaaaggaac cacgaggaag gaaagaaagg 901 cagccagatt aagtaaagaa gcattaaaac aactgcatag tgagactcag cgccttattc 961 gagagtctgc actgaacctt ccatatcata tgcctgagaa taaaaccatt catgatttct 1021 tcaaacgtaa accccggccc acttgccacg gaaatgccat ggcactattg aagtcatcta 1081 aatatcagtc aagccatcac aaagaaatca tagacactgc aaatactact gaaatgaaca 1141 gtgatcacca tagtaaaggt tctgagcaga caacaggtgc agaaaatgaa gtggaaacta 1201 atgcactccc tgtagtttca aaggaaaccc agatcattac tggatcagat gagtcttgca 1261 ggaaggattt ggtaaaaaat gaagagctag aaattcagga gaaacagaag cagagtgaca 1321 ttagaccttc acctggggac agctcagtgt tgcaacagga atccaacttc ctcgggaaca 1381 atcacagtga ggaatgtcag gttgaagggc ttgtagcatt tgaacctcat gccctggagg 1441 gtgaaggccc ccaaaatcca gaagaaacag atgagaaagt ggaagagcct gagcagcaaa 1501 ataaatcatc agcagttggg ccacctgaaa aagtgagacg gtttactctg gatagactta 1561 agcaactggg agtagatgtt tccattaaac cacggctagg tgctgatgaa gattcctttg 1621 tgatacttga acctgaaacc aacagaggtg aaaagcttca ggtgttaaaa gctaaactgc 1681 aagaagcaat gaaactccga aggtttgagg agcgccagaa gcgccaagca ctgtttaaat 1741 tagataatga agatgggttt gaggaagagg aggaggaaga ggaagaaatg acagatgagt 1801 ctgaggaaga tggagaagag aaggtagaga aagaagagaa agaggaagaa ctagaggaag 1861 aggaggagaa agaagaggag gaggaagaag aaggaaatca ggagactgca gaattccttc 1921 ttagtagtga agaaatagaa acaaaagatg aaaaagaaat ggataaagaa aataatgatg 1981 gcagtagtga aattggcaag gcagttggct tcctctctgt tcccaagtct ctctcatcag 2041 attctacttt acttctgttt aaggacagct cttccaagat gggttacttt cctactgaag 2101 aaaaatcaga aacagatgaa aactcaggca agcagcctag caaactggat gaggatgatt 2161 catgttcatt gctaacaaag gagagcagcc acaatagcag ctttgagctg attggctcca 2221 cgattccatc ctatcagcct tgcaacagac aaacaggccg tgggaccagt tttttcccta 2281 cagcaggagg attcagatct ccttcccctg ggctatttcg agccagtttg gtcagctcag 2341 cttctaagag ttcagggaaa ctgtctgagc cttcacttcc catagaggat tcccaggatc 2401 tgtataacgc ctccccagag cctaagacac ttttcctagg agcaggagac ttccagttct 2461 gtttagaaga tgacactcag agccaactgt tggatgcaga tgggttctta aatgttagaa 2521 accacaggaa tcagtaccaa gctttgaagc ctcgattgcc attggccagt atggatgaga 2581 atgccatgga tgccaacatg gatgagctgt tggatttgtg tactggaaag ttcacatctc 2641 aggctgaaaa acatctaccc aggaagggtg acgagaaaga gaacatggag gaacttctga 2701 acctttgttc aggaaaattc acttctcagg atgcctccac tccagcctca tcagagttaa 2761 ataaacagga gaaggagagc agcatgggtg atccaatgga agaagcactt gctctttgct 2821 caggctcttt tcccacagac aaggaagagg aagacgagga ggaggaattt ggagactttc 2881 ggcttgtttc aaatgataat gagtttgata gtgatgagga tgaacacagt gactctggta 2941 atgatctggc actggaagac catgaagatg atgatgaaga agaactcctg aagcgatctg 3001 agaagttgaa aaggcaaatg aggttgagga aatacctgga ggatgaggca gaggtgtcag 3061 gaagtgatgt gggaagcgaa gatgagtatg atggggaaga aattgatgaa tatgaagagg 3121 acgtaattga tgaagtactt ccttctgatg aggaactgca gagtcaaatc aagaaaatac 3181 acatgaaaac tatgttggat gatgataagc gacagctacg tttataccaa gagaggtacc 3241 ttgctgatgg ggatctacac agcgatggtc ctgggcgaat gaggaagttt cgatggaaaa 3301 acatagatga tgcttcccag atggacttgt tccacagaga ctctgatgat gatcagactg 3361 aagaacagct tgatgagtca gaagccaggt ggaggaagga gcgaattgaa cgagagcagt 3421 ggcttcggga catggcacag caggggaaaa ttacagctga agaagaagaa gaaattgggG 3481 AGGACAGTCA GTTTATGATA CTGGCCAAGA AAGTTACAGC CAAAGCACTG CAGAAGAATG 3541 CCAGTCGCCC TATGGTTATT CAGGAATCAA AGTCTTTGCT CAGAAATCCT TTTGAAGCCA 3601 TCAGACCAGG AAGTGCTCAA CAGGTGAAGA CAGGCTCACT GCTAAACCAG CCCAAAGCTG 3661 TGCTTCAGAA ACTGGCTGCT CTCTCTGACC ATAACCCCAG TGCTCCTCGA AATTCGAGAA 3721 ACTTTGTCTT TCATACACTT TCTCCTGTCA AGGCTGAGGC GGCAAAGGAA TCGTCTAAGT 3781 CTCAGGTAAA GAAAAGGGGT CCATCTTTCA TGACTTCTCC TTCACCTAAG CACCTCAAAA 3841 CAGATGATAG CACTTCAGGA TTGACGCGAA GCATCTTCAA ATATTTGGAG AGCTTGCCAA 3901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 4021 TATCTTGTGG AAAGGACGAA GATCCGGCGT TCGCGACTTC ATGACGCGTT AAGTCgacaa 4081 tcaacctctg gattacaaaa tttgtgaaag att