Construct: ORF TRCN0000479304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012853.1_s317c1
- Derived from:
- ccsbBroadEn_07870
- DNA Barcode:
- ATACAAATCGATTCTACTCCTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANGEL1 (23357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23357 | ANGEL1 | angel homolog 1 | NM_015305.4 | 99.9% | 99.8% | 1244A>G |
| 2 | human | 23357 | ANGEL1 | angel homolog 1 | NM_001370748.1 | 92.8% | 89.1% | (many diffs) |
| 3 | human | 23357 | ANGEL1 | angel homolog 1 | NM_001370747.1 | 92.6% | 92.5% | 1244A>G;1380_1538del |
| 4 | human | 23357 | ANGEL1 | angel homolog 1 | NM_001370746.1 | 86.3% | 82.9% | (many diffs) |
| 5 | mouse | 68737 | Angel1 | angel homolog 1 | NM_144524.2 | 89% | 90.4% | (many diffs) |
| 6 | mouse | 68737 | Angel1 | angel homolog 1 | XM_006516199.3 | 86.4% | 85.6% | (many diffs) |
| 7 | mouse | 68737 | Angel1 | angel homolog 1 | XM_011244169.1 | 85.7% | 83.8% | (many diffs) |
| 8 | mouse | 68737 | Angel1 | angel homolog 1 | XM_006516200.3 | 82.8% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2076
- ORF length:
- 2010
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cgcgtcgtgc ttgtgttacc tgctgctgcc ggccacgcgc ctcttccgcg 121 ccctctcaga tgctttcttc acatgtcgaa aaaatgtcct tctggcgaac agctcatccc 181 cccaggtaga gggcgacttt gccatggccc ctcggggccc tgagcaggag gaatgtgagg 241 gcctgctgca gcagtggcga gaagaagggt tgagccaggt gctctcaact gcaagtgagg 301 ggccccttat agataaagga ctagcccaga gcagcctggc acttctgatg gataatcctg 361 gagaagagaa tgctgcttca gaggacaggt ggtccagcag gcagctgagt gaccttcggg 421 ctgcagagaa cctggatgag cctttccctg agatgctagg agaggagcca ctgctggagg 481 tggagggggt ggagggctcc atgtgggcag ctatccccat gcagtcggag ccccagtatg 541 cagactgtgc tgccctccca gtgggtgccc tggccacaga gcagtgggaa gaggacccag 601 cggtgttggc ctggagcata gcacctgagc ctgtgcccca ggaagaggct tccatctggc 661 cctttgaggg cctggggcag ttgcagcctc ccgcagtgga aataccatat catgaaattt 721 tgtggcgaga atgggaggat ttctccaccc agccagatgc tcagggcctg aaggcaggag 781 atggccctca gttccagttc actctgatgt cttataacat cctggctcag gacctgatgc 841 agcagagctc agagctctat ctacattgcc atccagacat cctcaattgg aactatcgct 901 tcgtgaacct catgcaggaa ttccagcact gggaccctga tatcctgtgt ctccaggaag 961 tccaggaaga tcattactgg gagcagctgg aaccctctct gcgaatgatg ggctttacct 1021 gtttctacaa gaggaggact gggtgtaaaa ccgatggctg tgctgtctgc tacaagccta 1081 ccagattccg cctgctctgt gctagccctg tggagtactt ccggcctggc ttggagctac 1141 ttaatcggga taatgtgggc ttagtgttgc tactgcaacc actcgtccca gaaggcctgg 1201 gacaagtctc ggtggccccg ctgtgtgtgg caaataccca tatcctttac aacccacgcc 1261 ggggcgatgt caagctggcc cagatggcca ttctcctggc ggaagtgggc aaggtggcca 1321 gactgtcaga tggcagccac tgccccatca tcttgtgcgg ggacctaaat tctgtccctg 1381 attcacctct ctacaacttc atcagggatg gagagctcca gtaccatggg atgccagcct 1441 ggaaggtatc tggacaggaa gacttctccc atcagcttta ccagaggaag ctgcaggccc 1501 cactgtggcc cagctccctg ggcatcactg attgctgtca gtatgtcacc tcctgtcacc 1561 ccaagagatc agagagacgc aagtatggcc gagacttcct gctacgtttc cgcttctgca 1621 gcatcgcttg tcagcgacca gtaggactgg tccttatgga aggagtgaca gatactaagc 1681 cagagcgacc TGCGGGTTGG GCTGAGTCTG TCCTTGAGGA AGATGCATCG GAGCTTGAGC 1741 CTGCCTTCTC CAGGACTGTA GGTACCATCC AGCACTGCCT CCACCTGACG TCAGTATATA 1801 CCCACTTCCT GCCCCAGCGT GGCCGCCCAG AGGTCACTAC AATGCCATTG GGTCTTGGAA 1861 TGACAGTAGA TTACATCTTC TTCTCAGCTG AGTCCTGTGA GAATGGGAAC AGAACTGATC 1921 ACAGGCTGTA TCGAGATGGA ACTCTCAAGC TCCTGGGTCG TCTCTCCCTT CTCTCTGAAG 1981 AGATACTCTG GGCTGCCAAT GGCTTACCCA ACCCCTTCTG CTCTTCAGAC CACCTCTGCC 2041 TGCTAGCCAG CTTCGGGATG GAAGTCACCG CCCCATGCCC AACTTTCTTG TACAAAGTGG 2101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2221 AATACAAATC GATTCTACTC CTATAACGCG TTAAGTCgac aatcaacctc tggattacaa 2281 aatttgtgaa agatt