Construct: ORF TRCN0000479316

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007239.1_s317c1
Derived from:
ccsbBroadEn_09350
DNA Barcode:
AATACTTACCACCTTTATCTGGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SHKBP1 (92799)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479316
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92799 SHKBP1 SH3KBP1 binding protein 1 NM_138392.4 99.8% 99.8% 63T>C;1520A>T;1629A>G
2 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_017027473.2 99.7% 99.7% (many diffs)
3 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_006723474.2 98.4% 98.4% (many diffs)
4 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_006723475.2 98.3% 98.3% (many diffs)
5 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_011527492.2 84.7% 84.7% (many diffs)
6 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_017027474.2 76.8% 76.8% 0_1ins489;1031A>T;1140A>G
7 human 92799 SHKBP1 SH3KBP1 binding protein 1 XM_011527493.2 75.7% 75.7% (many diffs)
8 mouse 192192 Shkbp1 Sh3kbp1 binding protein 1 NM_138676.2 85.4% 92.6% (many diffs)
9 mouse 192192 Shkbp1 Sh3kbp1 binding protein 1 XM_006539667.2 85.3% 92.5% (many diffs)
10 mouse 192192 Shkbp1 Sh3kbp1 binding protein 1 XM_017322044.1 78.2% 84.7% (many diffs)
11 mouse 192192 Shkbp1 Sh3kbp1 binding protein 1 XR_881667.1 75.6% (many diffs)
12 mouse 192192 Shkbp1 Sh3kbp1 binding protein 1 XM_006539668.2 61.4% 66.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2187
ORF length:
2121
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agcagcggct actgcagccg agggggtccc cagtcggggg cctcccgggg
121aagtcatcca tctgaatgtg ggaggcaaga gattcagtac ctctcgccag actctcacct
181ggatcccaga ctccttcttc tccagtcttc tgagcggacg catctcgacg ctgaaagatg
241agaccggagc aatcttcatc gacagggacc ctacagtctt cgcccccatc ctcaacttcc
301tgcgcaccaa agagttggat cccaggggtg tccacggttc cagcctcctc catgaagccc
361agttctatgg gctcactcct ctggttcgtc gcctgcagct tcgagaggag ttggatcgat
421cttcttgtgg aaacgtcctc ttcaatggtt acctgccgcc accagtgttc ccagtgaagc
481ggcggaaccg gcacagccta gtggggcctc agcagctagg aggacggcca gcccctgtcc
541gacggagcaa cacgatgccc cccaaccttg gcaatgcagg gctgctgggc cgaatgctgg
601atgagaaaac ccctccctca ccctcaggac aacctgagga gccggggatg gtgcgcctgg
661tgtgtggaca ccataattgg atcgctgtgg cctataccca gtttctagtc tgctacaggt
721tgaaggaagc ctctggctgg cagctggtgt tttccagccc ccgcctggac tggcccatcg
781aacgactggc gctcacagcc cgggtgcatg gtggggcttt gggtgaacat gacaagatgg
841tggcagcagc caccggcagc gagatcctgc tatgggctct gcaggcggaa ggcggtggct
901ccgagatagg ggtctttcat ctgggggtgc ctgtggaggc cttgttcttc gtcgggaacc
961agctcattgc tacaagccac acagggcgca tcggggtgtg gaatgccgtc accaagcact
1021ggcaggtcca ggaggtgcag cccatcacca gttatgacgc ggcaggctcc ttcctcctcc
1081tgggctgcaa caacggctcc atttactacg tggatgtgca gaagttcccc ttgcgcatga
1141aagacaacga cctccttgtc agcgagctct atcgggaccc agcggaggat ggggtcaccg
1201ccctcagtgt ctacctcacc cccaagacca gtgacagtgg gaactggatc gagatcgcct
1261atggcaccag ctcagggggc gtgcgggtca tcgtgcagca cccggagact gtgggctcgg
1321ggcctcagct cttccagacc ttcactgtgc accgcagccc tgtcaccaag atcatgctgt
1381cggagaagca cctcatctca gtctgtgccg acaacaacca cgtgcggaca tggtctgtga
1441ctcgcttccg cggcatgatt tccacccagc ccggctccac cccactcgct tcctttaaga
1501tcctggctct ggagtcggca gatgggcatg gcggctgcag tgctggcaat gacattggcc
1561cctacggtga gcgggacgac cagctagtgt tcatccagaa ggtggtgccc agtgccagcc
1621agctcttcgt gcgtctctca tctactgggc agcgggtgtg ctccgtgcgc tccgtggacg
1681gctcacccac gacggccttc acagtgctgg agtgcgaggg ctcccggcgg ctcggctctc
1741ggccccggcg ctacctgctc actggccagg ccaacggcag cttggccatg tgggacctaa
1801ccaccgccat ggacggccTC GGCCAGGCCC CTGCAGGTGG CCTGACGGAG CAAGAGCTGA
1861TGGAACAGCT GGAACACTGT GAGCTGGCCC CGCCGGCTCC TTCAGCTCCC TCATGGGGCT
1921GTCTCCCCAG CCCCTCACCC CGCATCTCCC TCACCAGCCT CCACTCAGCC TCCAGCAACA
1981CCTCCTTGTC TGGCCACCGT GGGAGCCCAA GCCCCCCGCA GGCTGAGGCC CGGCGCCGTG
2041GTGGGGGCAG CTTTGTGGAA CGCTGCCAGG AACTGGTGCG GAGTGGGCCA GACCTCCGAC
2101GGCCACCCAC ACCAGCCCCG TGGCCCTCCA GCGGTCTCGG CACTCCCCTC ACACCTCCCA
2161AGATGAAGCT CAATGAAACT TCCTTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
2221GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2281AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATACTTA
2341CCACCTTTAT CTGGTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2401aagatt

Download FASTA (ORF) (Full)