Construct: ORF TRCN0000479316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007239.1_s317c1
- Derived from:
- ccsbBroadEn_09350
- DNA Barcode:
- AATACTTACCACCTTTATCTGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHKBP1 (92799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | NM_138392.4 | 99.8% | 99.8% | 63T>C;1520A>T;1629A>G |
| 2 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_017027473.2 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_006723474.2 | 98.4% | 98.4% | (many diffs) |
| 4 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_006723475.2 | 98.3% | 98.3% | (many diffs) |
| 5 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_011527492.2 | 84.7% | 84.7% | (many diffs) |
| 6 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_017027474.2 | 76.8% | 76.8% | 0_1ins489;1031A>T;1140A>G |
| 7 | human | 92799 | SHKBP1 | SH3KBP1 binding protein 1 | XM_011527493.2 | 75.7% | 75.7% | (many diffs) |
| 8 | mouse | 192192 | Shkbp1 | Sh3kbp1 binding protein 1 | NM_138676.2 | 85.4% | 92.6% | (many diffs) |
| 9 | mouse | 192192 | Shkbp1 | Sh3kbp1 binding protein 1 | XM_006539667.2 | 85.3% | 92.5% | (many diffs) |
| 10 | mouse | 192192 | Shkbp1 | Sh3kbp1 binding protein 1 | XM_017322044.1 | 78.2% | 84.7% | (many diffs) |
| 11 | mouse | 192192 | Shkbp1 | Sh3kbp1 binding protein 1 | XR_881667.1 | 75.6% | (many diffs) | |
| 12 | mouse | 192192 | Shkbp1 | Sh3kbp1 binding protein 1 | XM_006539668.2 | 61.4% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcagcggct actgcagccg agggggtccc cagtcggggg cctcccgggg 121 aagtcatcca tctgaatgtg ggaggcaaga gattcagtac ctctcgccag actctcacct 181 ggatcccaga ctccttcttc tccagtcttc tgagcggacg catctcgacg ctgaaagatg 241 agaccggagc aatcttcatc gacagggacc ctacagtctt cgcccccatc ctcaacttcc 301 tgcgcaccaa agagttggat cccaggggtg tccacggttc cagcctcctc catgaagccc 361 agttctatgg gctcactcct ctggttcgtc gcctgcagct tcgagaggag ttggatcgat 421 cttcttgtgg aaacgtcctc ttcaatggtt acctgccgcc accagtgttc ccagtgaagc 481 ggcggaaccg gcacagccta gtggggcctc agcagctagg aggacggcca gcccctgtcc 541 gacggagcaa cacgatgccc cccaaccttg gcaatgcagg gctgctgggc cgaatgctgg 601 atgagaaaac ccctccctca ccctcaggac aacctgagga gccggggatg gtgcgcctgg 661 tgtgtggaca ccataattgg atcgctgtgg cctataccca gtttctagtc tgctacaggt 721 tgaaggaagc ctctggctgg cagctggtgt tttccagccc ccgcctggac tggcccatcg 781 aacgactggc gctcacagcc cgggtgcatg gtggggcttt gggtgaacat gacaagatgg 841 tggcagcagc caccggcagc gagatcctgc tatgggctct gcaggcggaa ggcggtggct 901 ccgagatagg ggtctttcat ctgggggtgc ctgtggaggc cttgttcttc gtcgggaacc 961 agctcattgc tacaagccac acagggcgca tcggggtgtg gaatgccgtc accaagcact 1021 ggcaggtcca ggaggtgcag cccatcacca gttatgacgc ggcaggctcc ttcctcctcc 1081 tgggctgcaa caacggctcc atttactacg tggatgtgca gaagttcccc ttgcgcatga 1141 aagacaacga cctccttgtc agcgagctct atcgggaccc agcggaggat ggggtcaccg 1201 ccctcagtgt ctacctcacc cccaagacca gtgacagtgg gaactggatc gagatcgcct 1261 atggcaccag ctcagggggc gtgcgggtca tcgtgcagca cccggagact gtgggctcgg 1321 ggcctcagct cttccagacc ttcactgtgc accgcagccc tgtcaccaag atcatgctgt 1381 cggagaagca cctcatctca gtctgtgccg acaacaacca cgtgcggaca tggtctgtga 1441 ctcgcttccg cggcatgatt tccacccagc ccggctccac cccactcgct tcctttaaga 1501 tcctggctct ggagtcggca gatgggcatg gcggctgcag tgctggcaat gacattggcc 1561 cctacggtga gcgggacgac cagctagtgt tcatccagaa ggtggtgccc agtgccagcc 1621 agctcttcgt gcgtctctca tctactgggc agcgggtgtg ctccgtgcgc tccgtggacg 1681 gctcacccac gacggccttc acagtgctgg agtgcgaggg ctcccggcgg ctcggctctc 1741 ggccccggcg ctacctgctc actggccagg ccaacggcag cttggccatg tgggacctaa 1801 ccaccgccat ggacggccTC GGCCAGGCCC CTGCAGGTGG CCTGACGGAG CAAGAGCTGA 1861 TGGAACAGCT GGAACACTGT GAGCTGGCCC CGCCGGCTCC TTCAGCTCCC TCATGGGGCT 1921 GTCTCCCCAG CCCCTCACCC CGCATCTCCC TCACCAGCCT CCACTCAGCC TCCAGCAACA 1981 CCTCCTTGTC TGGCCACCGT GGGAGCCCAA GCCCCCCGCA GGCTGAGGCC CGGCGCCGTG 2041 GTGGGGGCAG CTTTGTGGAA CGCTGCCAGG AACTGGTGCG GAGTGGGCCA GACCTCCGAC 2101 GGCCACCCAC ACCAGCCCCG TGGCCCTCCA GCGGTCTCGG CACTCCCCTC ACACCTCCCA 2161 AGATGAAGCT CAATGAAACT TCCTTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATACTTA 2341 CCACCTTTAT CTGGTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt