Construct: ORF TRCN0000479320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006300.1_s317c1
- Derived from:
- ccsbBroadEn_12095
- DNA Barcode:
- ACTCCCAGGACAATCGTCTAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC49 (54839)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54839 | LRRC49 | leucine rich repeat contain... | NM_017691.4 | 99.8% | 99.8% | 1054_1056delCAA |
| 2 | human | 54839 | LRRC49 | leucine rich repeat contain... | NM_001199017.2 | 99.1% | 99.1% | 106_120del;1069_1071delCAA |
| 3 | human | 54839 | LRRC49 | leucine rich repeat contain... | NM_001284357.1 | 98.1% | 97.6% | (many diffs) |
| 4 | human | 54839 | LRRC49 | leucine rich repeat contain... | XM_024449965.1 | 97.4% | 96.9% | (many diffs) |
| 5 | human | 54839 | LRRC49 | leucine rich repeat contain... | NM_001199018.2 | 92.2% | 91.1% | (many diffs) |
| 6 | human | 54839 | LRRC49 | leucine rich repeat contain... | XM_011521715.3 | 92.2% | 91.1% | (many diffs) |
| 7 | human | 54839 | LRRC49 | leucine rich repeat contain... | XM_017022358.1 | 92.2% | 91.1% | (many diffs) |
| 8 | human | 54839 | LRRC49 | leucine rich repeat contain... | NM_001363732.1 | 56.9% | 56.9% | 0_1ins882;172_174delCAA |
| 9 | human | 54839 | LRRC49 | leucine rich repeat contain... | XM_005254492.4 | 56.9% | 56.9% | 0_1ins882;172_174delCAA |
| 10 | human | 54839 | LRRC49 | leucine rich repeat contain... | XM_011521717.2 | 56.9% | 56.9% | 0_1ins882;172_174delCAA |
| 11 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | NM_001146046.1 | 84.9% | 89.5% | (many diffs) |
| 12 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | NM_001311095.1 | 83.2% | 87.2% | (many diffs) |
| 13 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | NM_145616.4 | 77.5% | 81.6% | (many diffs) |
| 14 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | NM_001146047.1 | 75.9% | 79.5% | (many diffs) |
| 15 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | XR_001778785.1 | 53.4% | (many diffs) | |
| 16 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | XM_006510754.3 | 47.2% | 50.7% | (many diffs) |
| 17 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | XM_017313093.1 | 47.2% | 50.7% | (many diffs) |
| 18 | mouse | 102747 | Lrrc49 | leucine rich repeat contain... | XM_017313094.1 | 47.2% | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2121
- ORF length:
- 2055
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tcccgggaaa tatcgctctg tttctggccg ggctgcgaac aacgtgaact 121 gcgggcttca tctggttatt caaacatcat cgcttcctga aaaaaacaaa gttgaattca 181 agctaaataa agacacatcg tcattccccg gtagactttt acaacatgac cttgaaagaa 241 actactcaag taggcaaggt gatcatatta atttggtgag ctcatcattg tcatcatttc 301 ctattcttca acgttcttct gaagagaaaa ttctttactc agacaggttg agcctagaaa 361 gacaaaagct gaccgtatgt cctatcatca atggggaaga ccaccttcgt ttgttgaact 421 ttcaacacaa ttttataact cggatacaaa atatttctaa tctacagaag ttaatatcgt 481 tggatttata tgataaccag attgaagaaa ttagtgggct ttcgactctg agatgtcttc 541 gtgtccttct gttggggaaa aacagaatca agaaaatctc aaatctggag aatctaaaaa 601 gcttagatgt cttggatctt catggaaatc agattaccaa aattgaaaat attaatcatt 661 tgtgtgagtt gagagtttta aatcttgcca ggaacttttt aagtcatgtt gataatctta 721 atgggctgga ttcactaact gaacttaact tgcgacacaa tcaaatcact ttcgtgagag 781 atgtggataa tttgccctgc ctccaacatc tctttctcag ctttaacaat atatctagtt 841 ttgacagtgt ttcctgcctt gctgactctt cttccctctc ggacatcacc tttgatggca 901 atcccatagc tcaagagtca tggtacaaac acactgtcct tcagaatatg atgcagctgc 961 gccagctaga tatgaagaga atcacggaag aagaaaggcg tatggcatct gttttagcca 1021 aaaaagagga agagaagaag cgggaaagtc ataaacaatc tttgcttaag gagaagaaaa 1081 ggttaacaat taacaacgta gctcgacagt gggacttgca acaacgagta gccaatattg 1141 ctacaaatga agatagaaaa gattctgact ctcctcagga cccctgtcag attgatggaa 1201 gcaccctctc tgcattccca gaggaaacag ggcctctaga ctcaggactc aacaatgctt 1261 tacaaggttt atctgtcata gacacatacc ttgttgaagt ggacggggat acactttccc 1321 tatatggctc aggagcactg gaatctctgg ataggaattg gagtgttcaa acagcaggaa 1381 tgatcacaac agtctccttc actttcatag aatttgatga aatcgtccaa gtgcttccta 1441 aactgaagat taagtttcct aattctctgc accttaaatt caaggaaaca aatcttgtaa 1501 tgctgcagca atttaacgca ctagcccaac tccgtcgtat tgaccagttg acaattgatc 1561 ctcaaggaaa tccagttgtc aattttacac tctggaaata ctatgtactg tttaggctaa 1621 gccatttcag tatgcagaaa ataaatggaa cagaggtgac acagaatgat atgataatgg 1681 ctgaaaggct ctttggaatc ctagcacatg tagcatcttc tgagttaccc CAGTATCGTC 1741 TGATTTCCAT TCTGGGTGAT GCCAGGAAAA AGCAATTTCG GTATCTACTA GAATCCAAAG 1801 GAAAAAAACC TGGTATTATC AACGAAGAAA ATAATGACAG CAAAAGACTT GTAGGAGAAA 1861 ACACAAATCG TGCTACATTA AATTATACTA CAAGAGACTT TTATAATGAA AAGCTAGAGG 1921 AAATAAAGGA AAAGAAGAAA TTCTGTAAAA CATATATAGA AGACCTTGTG AAGGAAGCCA 1981 CAGAAATCAA CATGAAAAAT GAGGCTTTGC AGAAGCTTTG GCCACAGATG TTCATTGAGC 2041 TTGTTAGGGA TGCAGTCATA GAAATTCGCA ATAAAAATTC CTATATGAAG CTCTGCCTAC 2101 AGCAGATAAC AGACCAAAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2161 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2221 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTC CCAGGACAAT 2281 CGTCTAACCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt