Construct: ORF TRCN0000479328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005174.1_s317c1
- Derived from:
- ccsbBroadEn_06815
- DNA Barcode:
- AGCCGCAAATCCCAATTCCCGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN14 (5784)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5784 | PTPN14 | protein tyrosine phosphatas... | NM_005401.5 | 99.9% | 100% | 978A>G;3252A>G |
| 2 | human | 5784 | PTPN14 | protein tyrosine phosphatas... | XM_017001941.1 | 99.9% | 100% | 978A>G;3252A>G |
| 3 | human | 5784 | PTPN14 | protein tyrosine phosphatas... | XM_024448759.1 | 99.9% | 100% | 978A>G;3252A>G |
| 4 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | NM_008976.2 | 85.6% | 91.4% | (many diffs) |
| 5 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | XM_006497129.3 | 85.6% | 91.4% | (many diffs) |
| 6 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | XM_006497130.3 | 85.6% | 91.4% | (many diffs) |
| 7 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | XM_006497131.3 | 85.6% | 91.4% | (many diffs) |
| 8 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | XM_006497133.3 | 85.6% | 91.4% | (many diffs) |
| 9 | mouse | 19250 | Ptpn14 | protein tyrosine phosphatas... | XM_006497134.3 | 78% | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3630
- ORF length:
- 3561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccttttggt ctgaagctcc gccggacacg gcgctacaac gtcctgagca 121 agaactgctt tgtcacacgg attcgcctgc tggacagcaa tgttatcgag tgcacgctgt 181 cggtggaaag cacagggcaa gaatgcctgg aggctgtggc ccagaggctg gagctgcgag 241 agacgcacta ctttggcctt tggtttctca gcaagagcca gcaagcacga tgggtggagc 301 tggagaaacc tctgaagaaa catctggaca aattcgctaa tgagcctttg cttttctttg 361 gagtcatgtt ctatgtgcca aatgtgtcat ggcttcagca agaggccaca agatatcagt 421 attacctgca agtcaaaaaa gatgtgcttg aagggcgatt acgatgtaca ttggaccagg 481 tgattcggct agccggccta gctgtgcaag ctgattttgg agactataat cagtttgatt 541 ctcaagattt cctcagagag tatgtgctat ttcctatgga tttggccctg gaagaggctg 601 ttctggagga gctgacccag aaggtagccc aagaacacaa agcccacagt ggaatcctgc 661 cagcagaagc tgaactgatg tacatcaatg aagttgaacg tttggatgga tttggacagg 721 aaatcttccc tgtaaaggac aatcatggaa actgtgtaca ccttggcatt ttctttatgg 781 ggattttcgt gaggaacaga attggaagac aagcggtaat atacaggtgg aatgacatgg 841 ggaatatcac tcataacaag tcgaccattc tagtggagct catcaacaaa gaagagactg 901 ccctctttca cacggatgat atcgaaaatg ccaagtatat ttctcggttg tttgccacac 961 gacacaagtt ttacaaacaa aacaaaatct gcactgaaca gtcaaattct ccacccccca 1021 tcagacgcca gcccacctgg agccggtcct ctctgcccag gcagcagccg tacatcctgc 1081 ctcccgttca cgtccagtgt ggtgagcact actcggaaac gcacacctcg caagacagca 1141 tttttcatgg gaatgaagaa gccttgtatt gcaactctca caacagcctg gacttaaatt 1201 atttaaatgg cactgtcacc aatggcagcg tgtgtagcgt tcacagcgtc aactccctca 1261 actgctcgca aagtttcatc caggcctccc ctgtatcctc caacctcagt atccctggga 1321 gtgacatcat gcgggccgac tacatcccga gccaccggca cagcgcgatc atcgtgccct 1381 cgtacaggcc aacccccgat tatgagacag tcatgcgcca gatgaagagg gggatcctgc 1441 atacagacag ccagagccag tctctgagaa acctcaacat tatcaacacc catgcctaca 1501 accagccaga ggatctggtg tacagccaac cggagatgcg ggagaggcac ccctacactg 1561 tcccttatgg gccacagggg gtctacagca acaaacttgt cagtccatct gaccagagga 1621 acccaaagaa taatgtggta ccaagcaagc cgggggcaag cgccatctcg cacacggtga 1681 gcaccccaga gctggccaac atgcagctgc agggcagcca taactacagc acggcccaca 1741 tgcttaagaa ctatctcttc aggccaccgc ccccctaccc acggccacga cctgccacca 1801 gcaccccaga cctggccagc caccgccaca agtacgtcag cggcagcagc ccggacctgg 1861 tgacccggaa ggtgcagctc tcggtgaaga ccttccaaga ggacagctct ccggtggttc 1921 atcagtctct ccaggaggtg agcgagcccc tcacggccac caagcaccac ggcactgtga 1981 acaagcgcca cagcctggag gtgatgaaca gcatggtgcg gggcatggag gccatgacgc 2041 tcaagtcgct ccacctcccc atggctcgcc gcaacacgct ccgggagcag ggaccgcccg 2101 aggaggggtc aggcagccac gaggtccccc agctccctca gtatcaccac aagaagacct 2161 tctctgatgc cactatgcta atccacagca gcgagagtga ggaggaggag gaggaggctc 2221 cagaatcggt gccccagatc cccatgctcc gggagaagat ggagtacagt gcccagctgc 2281 aggcggccct ggcccgcatc cccaacaagc ccccgcctga gtaccccggt ccaaggaaga 2341 gtgtgagcaa tggggctctg aggcaggacc aagccagcct tcctcccgcc atggccagag 2401 ccagggtgct gaggcatggg ccagccaagg ccatcagcat gtctcggact gacccgccgg 2461 ctgtcaacgg ggcctctctc ggcccatcca tctcggaacc cgacctgact agtgtgaagg 2521 agcgggtcaa aaaagagcct gtgaaggaga gacctgtgtc tgaaatgttt tccctggaag 2581 acagcattat agagagagag atgatgatca ggaatctaga gaagcagaag atggcaggcc 2641 tggaggcaca gaagaggccg ctgatgttgg cagcattgaa tgggctctcg gtggctcgag 2701 tctcagggcg ggaagagaat cgagttgatg ccacccgggt tcccatggac gagaggttca 2761 gaaccctgaa gaagaaacta gaagagggaa tggtgttcac agaatatgag caaattccaa 2821 agaaaaaggc gaatggcatt ttcagcacag cagctctgcc agaaaacgcc gagcgcagcc 2881 gaatccgtga agttgtcccc tatgaggaga atcgagtaga gctgatacca accaaagaaa 2941 ataacacagg atacattaat gcctcccaca tcaaggtggt ggttggcggg gcagaatggc 3001 actacatagc cacccagggg cccctgccac acacgtgcca cgacttctgg cagatggtgt 3061 gggagcaggg agtgaatgtg attgccatgg tcactgcaga ggaggagggt ggacgaacca 3121 aaagccaccg atactggccc aaactaggtt caaagcacag ctcagccacc TATGGCAAGT 3181 TCAAGGTCAC CACGAAGTTT CGAACGGATT CTGTTTGCTA TGCAACCACG GGCTTGAAGG 3241 TCAAGCACCT TTTGTCTGGG CAAGAAAGGA CGGTGTGGCA TTTACAATAT ACTGACTGGC 3301 CAGATCACGG CTGTCCAGAG GATGTCCAAG GATTTTTATC CTACTTGGAG GAGATCCAGT 3361 CGGTCCGTCG CCATACCAAC AGCATGCTGG AAGGCACCAA GAACCGGCAC CCGCCCATCG 3421 TGGTCCACTG TAGTGCTGGG GTGGGAAGGA CCGGCGTGCT CATTCTTTCT GAGCTGATGA 3481 TCTACTGCTT GGAACATAAC GAAAAGGTGG AAGTGCCCAT GATGCTGAGG CTCCTCAGGG 3541 AGCAGAGGAT GTTCATGATC CAGACTATCG CTCAGTACAA GTTTGTCTAC CAAGTCCTCA 3601 TCCAGTTCCT CCAAAACTCC AGACTCATTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 3661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCCG 3781 CAAATCCCAA TTCCCGTCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3841 tgaaagatt