Construct: ORF TRCN0000479329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004679.1_s317c1
- Derived from:
- ccsbBroadEn_00007
- DNA Barcode:
- GTGTCCAAAATTAAGTGTATACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACADVL (37)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | NM_000018.4 | 100% | 100% | |
| 2 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XM_006721516.3 | 97.1% | 96% | (many diffs) |
| 3 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | NM_001033859.2 | 96.6% | 96.6% | 137_138ins66 |
| 4 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | NM_001270447.1 | 95.2% | 92.1% | (many diffs) |
| 5 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XM_011523830.2 | 94.8% | 91.3% | 1433_1434ins98;1506_1507insGGCA |
| 6 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XM_011523829.2 | 92% | 87.5% | (many diffs) |
| 7 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XM_024450741.1 | 91% | 92.7% | 1433_1434ins98;1507_1592del |
| 8 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XR_934021.2 | 89.2% | 1_59del;1663_1664insGGCA;2021_2192del | |
| 9 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | NM_001270448.1 | 88.3% | 88.3% | 0_1ins228 |
| 10 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XR_934022.2 | 85% | 1_59del;1492_1493ins98;1927_2098del | |
| 11 | human | 37 | ACADVL | acyl-CoA dehydrogenase very... | XR_934023.2 | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2031
- ORF length:
- 1965
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggcggctcgg atggccgcga gcttggggcg gcagctgctg aggctcgggg 121 gcggaagctc gcggctcacg gcgctcctgg ggcagccccg gcccggccct gcccggcggc 181 cctatgccgg gggtgccgct cagctggctc tggacaagtc agattcccac ccctctgacg 241 ctctgaccag gaaaaaaccg gccaaggcgg aatctaagtc ctttgctgtg ggaatgttca 301 aaggccagct caccacagat caggtgttcc catacccgtc cgtgctcaac gaagagcaga 361 cacagtttct taaagagctg gtggagcctg tgtcccgttt cttcgaggaa gtgaacgatc 421 ccgccaagaa tgacgctctg gagatggtgg aggagaccac ttggcagggc ctcaaggagc 481 tgggggcctt tggtctgcaa gtgcccagtg agctgggtgg tgtgggcctt tgcaacaccc 541 agtacgcccg tttggtggag atcgtgggca tgcatgacct tggcgtgggc attaccctgg 601 gggcccatca gagcatcggt ttcaaaggca tcctgctctt tggcacaaag gcccagaaag 661 aaaaatacct ccccaagctg gcatctgggg agactgtggc cgctttctgt ctaaccgagc 721 cctcaagcgg gtcagatgca gcctccatcc gaacctctgc tgtgcccagc ccctgtggaa 781 aatactatac cctcaatgga agcaagcttt ggatcagtaa tgggggccta gcagacatct 841 tcacggtctt tgccaagaca ccagttacag atccagccac aggagccgtg aaggagaaga 901 tcacagcttt tgtggtggag aggggcttcg ggggcattac ccatgggccc cctgagaaga 961 agatgggcat caaggcttca aacacagcag aggtgttctt tgatggagta cgggtgccat 1021 cggagaacgt gctgggtgag gttgggagtg gcttcaaggt tgccatgcac atcctcaaca 1081 atggaaggtt tggcatggct gcggccctgg caggtaccat gagaggcatc attgctaagg 1141 cggtagatca tgccactaat cgtacccagt ttggggagaa aattcacaac tttgggctga 1201 tccaggagaa gctggcacgg atggttatgc tgcagtatgt aactgagtcc atggcttaca 1261 tggtgagtgc taacatggac cagggagcca cggacttcca gatagaggcc gccatcagca 1321 aaatctttgg ctcggaggca gcctggaagg tgacagatga atgcatccaa atcatggggg 1381 gtatgggctt catgaaggaa cctggagtag agcgtgtgct ccgagatctt cgcatcttcc 1441 ggatctttga ggggacaaat gacattcttc ggctgtttgt ggctctgcag ggctgtatgg 1501 acaaaggaaa ggagctctct gggcttggca gtgctctaaa gaatcccttt gggaatgctg 1561 gcctcctgct aggagaggca ggcaaacagc tgaggcggcg ggcagggctg ggcagcggcc 1621 tgagtcTCAG CGGACTTGTC CACCCGGAGT TGAGTCGGAG TGGCGAGCTG GCAGTACGGG 1681 CTCTGGAGCA GTTTGCCACT GTGGTGGAGG CCAAGCTGAT AAAACACAAG AAGGGGATTG 1741 TCAATGAACA GTTTCTGCTG CAGCGGCTGG CAGACGGGGC CATCGACCTC TATGCCATGG 1801 TGGTGGTTCT CTCGAGGGCC TCAAGATCCC TGAGTGAGGG CCACCCCACG GCCCAGCATG 1861 AGAAAATGCT CTGTGACACC TGGTGTATCG AGGCTGCAGC TCGGATCCGA GAGGGCATGG 1921 CCGCCCTGCA GTCTGACCCC TGGCAGCAAG AGCTCTACCG CAACTTCAAA AGCATCTCCA 1981 AGGCCTTGGT GGAGCGGGGT GGTGTGGTCA CCAGCAACCC ACTTGGCTTC TGCCCAACTT 2041 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2101 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2161 CTTGTGGAAA GGACGAGTGT CCAAAATTAA GTGTATACCT ACGCGTTAAG TCgacaatca 2221 acctctggat tacaaaattt gtgaaagatt