Construct: ORF TRCN0000479329

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004679.1_s317c1
Derived from:
ccsbBroadEn_00007
DNA Barcode:
GTGTCCAAAATTAAGTGTATACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACADVL (37)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479329
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 37 ACADVL acyl-CoA dehydrogenase very... NM_000018.4 100% 100%
2 human 37 ACADVL acyl-CoA dehydrogenase very... XM_006721516.3 97.1% 96% (many diffs)
3 human 37 ACADVL acyl-CoA dehydrogenase very... NM_001033859.2 96.6% 96.6% 137_138ins66
4 human 37 ACADVL acyl-CoA dehydrogenase very... NM_001270447.1 95.2% 92.1% (many diffs)
5 human 37 ACADVL acyl-CoA dehydrogenase very... XM_011523830.2 94.8% 91.3% 1433_1434ins98;1506_1507insGGCA
6 human 37 ACADVL acyl-CoA dehydrogenase very... XM_011523829.2 92% 87.5% (many diffs)
7 human 37 ACADVL acyl-CoA dehydrogenase very... XM_024450741.1 91% 92.7% 1433_1434ins98;1507_1592del
8 human 37 ACADVL acyl-CoA dehydrogenase very... XR_934021.2 89.2% 1_59del;1663_1664insGGCA;2021_2192del
9 human 37 ACADVL acyl-CoA dehydrogenase very... NM_001270448.1 88.3% 88.3% 0_1ins228
10 human 37 ACADVL acyl-CoA dehydrogenase very... XR_934022.2 85% 1_59del;1492_1493ins98;1927_2098del
11 human 37 ACADVL acyl-CoA dehydrogenase very... XR_934023.2 82.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2031
ORF length:
1965
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca ggcggctcgg atggccgcga gcttggggcg gcagctgctg aggctcgggg
121gcggaagctc gcggctcacg gcgctcctgg ggcagccccg gcccggccct gcccggcggc
181cctatgccgg gggtgccgct cagctggctc tggacaagtc agattcccac ccctctgacg
241ctctgaccag gaaaaaaccg gccaaggcgg aatctaagtc ctttgctgtg ggaatgttca
301aaggccagct caccacagat caggtgttcc catacccgtc cgtgctcaac gaagagcaga
361cacagtttct taaagagctg gtggagcctg tgtcccgttt cttcgaggaa gtgaacgatc
421ccgccaagaa tgacgctctg gagatggtgg aggagaccac ttggcagggc ctcaaggagc
481tgggggcctt tggtctgcaa gtgcccagtg agctgggtgg tgtgggcctt tgcaacaccc
541agtacgcccg tttggtggag atcgtgggca tgcatgacct tggcgtgggc attaccctgg
601gggcccatca gagcatcggt ttcaaaggca tcctgctctt tggcacaaag gcccagaaag
661aaaaatacct ccccaagctg gcatctgggg agactgtggc cgctttctgt ctaaccgagc
721cctcaagcgg gtcagatgca gcctccatcc gaacctctgc tgtgcccagc ccctgtggaa
781aatactatac cctcaatgga agcaagcttt ggatcagtaa tgggggccta gcagacatct
841tcacggtctt tgccaagaca ccagttacag atccagccac aggagccgtg aaggagaaga
901tcacagcttt tgtggtggag aggggcttcg ggggcattac ccatgggccc cctgagaaga
961agatgggcat caaggcttca aacacagcag aggtgttctt tgatggagta cgggtgccat
1021cggagaacgt gctgggtgag gttgggagtg gcttcaaggt tgccatgcac atcctcaaca
1081atggaaggtt tggcatggct gcggccctgg caggtaccat gagaggcatc attgctaagg
1141cggtagatca tgccactaat cgtacccagt ttggggagaa aattcacaac tttgggctga
1201tccaggagaa gctggcacgg atggttatgc tgcagtatgt aactgagtcc atggcttaca
1261tggtgagtgc taacatggac cagggagcca cggacttcca gatagaggcc gccatcagca
1321aaatctttgg ctcggaggca gcctggaagg tgacagatga atgcatccaa atcatggggg
1381gtatgggctt catgaaggaa cctggagtag agcgtgtgct ccgagatctt cgcatcttcc
1441ggatctttga ggggacaaat gacattcttc ggctgtttgt ggctctgcag ggctgtatgg
1501acaaaggaaa ggagctctct gggcttggca gtgctctaaa gaatcccttt gggaatgctg
1561gcctcctgct aggagaggca ggcaaacagc tgaggcggcg ggcagggctg ggcagcggcc
1621tgagtcTCAG CGGACTTGTC CACCCGGAGT TGAGTCGGAG TGGCGAGCTG GCAGTACGGG
1681CTCTGGAGCA GTTTGCCACT GTGGTGGAGG CCAAGCTGAT AAAACACAAG AAGGGGATTG
1741TCAATGAACA GTTTCTGCTG CAGCGGCTGG CAGACGGGGC CATCGACCTC TATGCCATGG
1801TGGTGGTTCT CTCGAGGGCC TCAAGATCCC TGAGTGAGGG CCACCCCACG GCCCAGCATG
1861AGAAAATGCT CTGTGACACC TGGTGTATCG AGGCTGCAGC TCGGATCCGA GAGGGCATGG
1921CCGCCCTGCA GTCTGACCCC TGGCAGCAAG AGCTCTACCG CAACTTCAAA AGCATCTCCA
1981AGGCCTTGGT GGAGCGGGGT GGTGTGGTCA CCAGCAACCC ACTTGGCTTC TGCCCAACTT
2041TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2101CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2161CTTGTGGAAA GGACGAGTGT CCAAAATTAA GTGTATACCT ACGCGTTAAG TCgacaatca
2221acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)