Construct: ORF TRCN0000479332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001726.1_s317c1
- Derived from:
- ccsbBroadEn_03682
- DNA Barcode:
- AGTCTTGGGTAAGCCTTCCTAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NKRF (55922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55922 | NKRF | NFKB repressing factor | XM_011531365.2 | 100% | 100% | |
| 2 | human | 55922 | NKRF | NFKB repressing factor | NM_017544.5 | 88% | 88% | 1_282del |
| 3 | human | 55922 | NKRF | NFKB repressing factor | NR_163972.1 | 53% | 1_809del;2880_3903del | |
| 4 | mouse | 77286 | Nkrf | NF-kappaB repressing factor | NM_029891.2 | 91.1% | 94.7% | (many diffs) |
| 5 | mouse | 77286 | Nkrf | NF-kappaB repressing factor | XM_006541409.3 | 80.2% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2139
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaaaatt ctccaaatgg ctgaaggtat tgatattggg gagatgcctt 121 catatgatct ggtgctgtcc aaaccttcca aaggtcaaaa acgccacctc tcaacatgtg 181 atggtcaaaa tcctcctaaa aagcaagccg gttccaaatt ccatgcgaga cctcgttttg 241 agcctgtaca ttttgtagct agtagttcaa aagatgaaag acaggaagat ccttatggcc 301 ctcaaacaaa agaggtaaat gaacaaacac attttgccag catgccaaga gacatctacc 361 aagattatac tcaagactct ttcagtatac aagatgggaa ttctcagtat tgtgattcat 421 caggattcat tctcacaaaa gaccagcctg taacagccaa catgtatttt gacagtggga 481 accctgcccc aagcaccaca tcacagcagg caaactctca gtcaactcct gagccttcac 541 catcacagac atttcccgag tctgtggtag ccgagaagca gtattttatt gaaaaattaa 601 cggcgacaat ctggaagaac ctttctaatc cagaaatgac ttctggatct gataaaatta 661 attatacata tatgttaact cgttgtattc aggcgtgtaa gacaaatcct gagtatatat 721 atgctccttt aaaggaaatt cctcctgccg acatccccaa aaataaaaaa cttctaactg 781 atggctatgc ttgtgaagtt agatgccaaa atatctactt aactacaggt tatgctggca 841 gcaagaatgg gtccagggat cgagctacag agctagctgt aaaactcttg cagaaacgta 901 ttgaagttag agttgtccgg cggaaattca agcatacatt tggagaggac ctcgtggtgt 961 gtcagattgg catgtcctcc tatgaatttc ctccagctct gaagccacca gaagacctgg 1021 tggtgctggg taaagatgct tccgggcagc caatttttaa tgcttctgcc aaacactgga 1081 ccaattttgt cattacagaa aatgcaaatg atgcaattgg tatccttaac aattctgcct 1141 cattcaacaa gatgtcaatt gaatacaaat atgagatgat gccaaatcgc acatggcgtt 1201 gtcgagtgtt tttacaagat cactgcttag ctgaaggtta tggaaccaag aaaacaagta 1261 aacatgcagc tgccgacgag gctttgaaaa ttcttcaaaa aacacagccc acttatccat 1321 ctgtcaaaag ttcacaatgc catacaggct cttcacccag aggatctgga aagaagaaag 1381 atataaagga tcttgtagtt tatgagaatt cttcaaatcc cgtgtgcacg ctgaacgaca 1441 cagctcagtt taaccgaatg acagttgagt atgtctatga aaggatgaca ggcctccgct 1501 ggaaatgcaa agtgattcta gagagtgaag taattgcaga agcagttggg gtgaagaaaa 1561 ctgtcaaata tgaagctgct ggggaagctg tgaaaaccct caaaaagacc cagccaactg 1621 tcattaacaa cttgaagaaa ggagctgttg aagatgtgat ttcaagaaat gaaattcagg 1681 gccgctcagc agaggaggct tacaaacagc aaatcaaaga agataatatt ggaaaTCAGC 1741 TGCTGAGAAA GATGGGTTGG ACTGGTGGTG GTTTAGGTAA ATCTGGTGAG GGCATACGGG 1801 AGCCTATCTC AGTGAAAGAG CAGCATAAGC GGGAAGGGCT TGGTCTGGAT GTAGAGAGGG 1861 TGAATAAAAT TGCCAAGAGA GATATTGAAC AGATCATCAG AAACTACGCC CGCTCCGAGA 1921 GCCACACAGA TTTGACTTTC TCTAGAGAGC TGACTAATGA TGAACGGAAG CAAATACATC 1981 AGATTGCCCA GAAGTATGGT CTTAAGAGTA AGTCTCATGG GGTGGGCCAT GATAGGTACC 2041 TAGTGGTAGG TAGAAAAAGA CGGAAGGAAG ACCTACTAGA TCAGCTCAAA CAGGAAGGCC 2101 AAGTGGGCCA TTACGAGCTT GTTATGCCTC AAGCAAATTT GCCAACTTTC TTGTACAAAG 2161 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2221 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2281 ACGAAGTCTT GGGTAAGCCT TCCTAAAAAC GCGTTAAGTC gacaatcaac ctctggatta 2341 caaaatttgt gaaagatt