Construct: ORF TRCN0000479340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012200.1_s317c1
- Derived from:
- ccsbBroadEn_07324
- DNA Barcode:
- TTAAGGGAGGTTCCTAATAAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX18 (8886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8886 | DDX18 | DEAD-box helicase 18 | NM_006773.4 | 99.9% | 99.7% | 281C>G;2003T>A |
| 2 | mouse | 66942 | Ddx18 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_025860.3 | 81.1% | 85.9% | (many diffs) |
| 3 | mouse | 66942 | Ddx18 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006529794.3 | 73.9% | 79.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2076
- ORF length:
- 2010
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc acacctgccg atgaaactcc tgcgtaagaa gatcgagaag cggaacctca 121 aattgcggca gcggaaccta aagtttcagg gggcctcaaa tctgacccta tcggaaactc 181 aaaatggaga tgtatctgaa gaaacaatgg gaagtagaaa ggttaaaaaa tcaaaacaaa 241 agcccatgaa tgtgggctta tcagaaactc aaaatggagg catgtctcaa gaagcagtgg 301 gaaatataaa agttacaaag tctccccaga aatccactgt attaagcaat ggagaagcag 361 caatgcagtc ttccaattca gaatcaaaaa agaaaaagaa gaaaaagaga aaaatggtga 421 atgatgctga gcctgatacg aaaaaagcaa aaactgaaaa caaagggaaa tctgaagaag 481 aaagtgccga gactactaaa gaaacagaaa ataatgtgga gaagccagat aatgatgaag 541 atgagagtga ggtgcccagt ctgcccctgg gactgacagg agcttttgag gatacttcgt 601 ttgcttctct atgtaatctt gtcaatgaaa acactctgaa ggcaataaaa gaaatgggtt 661 ttacaaacat gactgaaatt cagcataaaa gtatcagacc acttctggaa ggcagggatc 721 ttctagcagc tgcaaaaaca ggcagtggta aaaccctggc ttttctcatc cctgcagttg 781 aactcattgt taagttaagg ttcatgccca ggaatggaac aggagtcctt attctctcac 841 ctactagaga actagccatg caaacctttg gtgttcttaa ggagctgatg actcaccacg 901 tgcataccta tggcttgata atgggtggca gtaacagatc tgctgaagca cagaaacttg 961 gtaatgggat caacatcatt gtggccacac caggccgtct gctggaccat atgcagaata 1021 ccccaggatt tatgtataaa aacctgcagt gtctggttat tgatgaagct gatcgtatct 1081 tggatgtggg gtttgaagag gaattaaagc aaattattaa acttttgcca acacgtagac 1141 agactatgct cttttctgcc acccaaactc gaaaagttga agacctggca aggatttctc 1201 tgaaaaagga gccattgtat gttggcgttg atgatgataa agcgaatgca acagtggatg 1261 gtcttgaaca gggatatgtt gtttgtcctt ctgaaaagag attccttctg ctctttacat 1321 tccttaagaa gaaccgaaag aagaagctta tggtcttctt ttcatcttgt atgtctgtga 1381 aataccacta tgagttgctg aactacattg atttgcccgt cttggccatt catggaaagc 1441 aaaagcaaaa taagcgtaca accacattct tccagttctg caatgcagat tcgggaacac 1501 tattgtgtac ggatgtggca gcgagaggac tagacattcc tgaagtcgac tggattgttc 1561 agtatgaccc tccggatgac cctaaggaat atattcatcg tgtgggtaga acagccagag 1621 gccTAAATGG GAGAGGGCAT GCCTTGCTCA TTTTGCGCCC AGAAGAATTG GGTTTTCTTC 1681 GCTACTTGAA ACAATCCAAG GTTCCATTAA GTGAATTTGA CTTTTCCTGG TCTAAAATTT 1741 CTGACATTCA GTCTCAGCTT GAGAAATTGA TTGAAAAGAA TTACTTTCTT CATAAGTCAG 1801 CCCAGGAAGC ATATAAGTCA TACATACGAG CCTATGATTC CCATTCTCTG AAACAGATCT 1861 TTAATGTTAA TAACCTAAAT TTGCCTCAGG TTGCTCTGTC ATTTGGTTTC AAGGTGCCTC 1921 CCTTCGTTGA TCTGAACGTC AACAGTAATG AAGGCAAGCA GAAAAAGCGA GGAGGTGGTG 1981 GTGGATTTGG CTACCAGAAA ACCAAGAAAG TTGAGAAATC CAAAATCTTT AAACACATTA 2041 GCAAGAAATC ATCTGACAGC AGGCAGTACT CTCACTGCCC AACTTACTTG TACAAAGTGG 2101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2221 ATTAAGGGAG GTTCCTAATA AGCGGACGCG TTAAGTCgac aatcaacctc tggattacaa 2281 aatttgtgaa agatt