Transcript: Mouse NM_025860.3

Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ddx18 (66942)
Length:
2249
CDS:
106..2088

Additional Resources:

NCBI RefSeq record:
NM_025860.3
NBCI Gene record:
Ddx18 (66942)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025860.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071086 CGTCAATGAGAACACCCTGAA pLKO.1 630 CDS 100% 4.050 3.240 N Ddx18 n/a
2 TRCN0000316378 CGTCAATGAGAACACCCTGAA pLKO_005 630 CDS 100% 4.050 3.240 N Ddx18 n/a
3 TRCN0000071083 CCTGGTCTAAAGTTTCTGATA pLKO.1 1736 CDS 100% 4.950 3.465 N Ddx18 n/a
4 TRCN0000316376 CCTGGTCTAAAGTTTCTGATA pLKO_005 1736 CDS 100% 4.950 3.465 N Ddx18 n/a
5 TRCN0000071085 CGAAACCTGAAGCTGCAAGAA pLKO.1 172 CDS 100% 4.950 3.465 N Ddx18 n/a
6 TRCN0000071084 GCTGACCGTATCTTGGATGTT pLKO.1 1078 CDS 100% 4.950 3.465 N Ddx18 n/a
7 TRCN0000316377 GCTGACCGTATCTTGGATGTT pLKO_005 1078 CDS 100% 4.950 3.465 N Ddx18 n/a
8 TRCN0000071087 CCTGAAGAGTTGGGTTTCCTT pLKO.1 1669 CDS 100% 3.000 2.100 N Ddx18 n/a
9 TRCN0000316446 CCTGAAGAGTTGGGTTTCCTT pLKO_005 1669 CDS 100% 3.000 2.100 N Ddx18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025860.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07324 pDONR223 100% 81.1% 86.1% None (many diffs) n/a
2 ccsbBroad304_07324 pLX_304 0% 81.1% 86.1% V5 (many diffs) n/a
3 TRCN0000479340 TTAAGGGAGGTTCCTAATAAGCGG pLX_317 22.8% 81.1% 85.9% V5 (many diffs) n/a
4 TRCN0000488316 GGGACAACGTGGCTATACCTCCAT pLX_317 15.5% 81.1% 86.1% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000487989 ACACGAGTGACATTGGTGGCGATC pLX_317 11.2% 81.1% 86% V5 (many diffs) n/a
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