Construct: ORF TRCN0000479389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011894.4_s317c1
- Derived from:
- ccsbBroadEn_06560
- DNA Barcode:
- CTCCATTCAAAATAGAAACCTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARK3 (4140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4140 | MARK3 | microtubule affinity regula... | NM_002376.6 | 100% | 100% | |
| 2 | human | 4140 | MARK3 | microtubule affinity regula... | XM_005267641.5 | 98.7% | 98.7% | 1843_1869del |
| 3 | human | 4140 | MARK3 | microtubule affinity regula... | NM_001128919.2 | 97.9% | 97.9% | 1843_1887del |
| 4 | human | 4140 | MARK3 | microtubule affinity regula... | NM_001128920.2 | 97.8% | 97.8% | 1108_1109ins48 |
| 5 | human | 4140 | MARK3 | microtubule affinity regula... | NM_001128918.3 | 96.8% | 96.8% | 1843_1914del |
| 6 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021292.2 | 96.6% | 96.6% | 1108_1109ins48;1795_1821del |
| 7 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021291.2 | 95.8% | 95.8% | 1108_1109ins48;1795_1839del |
| 8 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449583.1 | 94.8% | 94.5% | (many diffs) |
| 9 | human | 4140 | MARK3 | microtubule affinity regula... | XM_005267642.5 | 94.6% | 94.6% | 1108_1109ins48;1795_1866del |
| 10 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021293.1 | 94.5% | 94.3% | (many diffs) |
| 11 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021298.2 | 91.2% | 91.2% | 51_52ins192 |
| 12 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021296.2 | 90.1% | 90.1% | 51_52ins192;1651_1677del |
| 13 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021295.2 | 89.3% | 89.3% | 51_52ins192;1651_1695del |
| 14 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021294.2 | 88.3% | 88.3% | 51_52ins192;1651_1722del |
| 15 | human | 4140 | MARK3 | microtubule affinity regula... | XM_005267643.5 | 88.2% | 88.2% | 1584_1585ins258 |
| 16 | human | 4140 | MARK3 | microtubule affinity regula... | NM_001128921.2 | 88% | 88% | 537_538ins237;1606_1632del |
| 17 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021299.2 | 87.2% | 87.2% | 51_52ins192;916_917ins48;1603_1647del |
| 18 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021297.2 | 86.1% | 86.1% | 51_52ins192;916_917ins48;1603_1674del |
| 19 | human | 4140 | MARK3 | microtubule affinity regula... | XM_011536769.3 | 86.1% | 86% | 0_1ins241;2delT;1603_1674del |
| 20 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449587.1 | 82% | 82% | 0_1ins393 |
| 21 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449586.1 | 81% | 81% | 0_1ins393;1450_1476del |
| 22 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449585.1 | 80.3% | 80.3% | 0_1ins393;1450_1494del |
| 23 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021301.2 | 79.4% | 79.4% | 0_1ins393;1450_1521del |
| 24 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449584.1 | 79.4% | 79.4% | 0_1ins393;1450_1521del |
| 25 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021308.2 | 78.8% | 78.8% | 0_1ins393;715_716ins48;1402_1428del |
| 26 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449590.1 | 78.3% | 77.9% | (many diffs) |
| 27 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449591.1 | 78.3% | 77.9% | (many diffs) |
| 28 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449588.1 | 78.2% | 78.2% | 0_1ins393;715_716ins48;1402_1446del |
| 29 | human | 4140 | MARK3 | microtubule affinity regula... | XM_024449589.1 | 76.7% | 76.3% | (many diffs) |
| 30 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021306.2 | 75.8% | 75.4% | (many diffs) |
| 31 | human | 4140 | MARK3 | microtubule affinity regula... | XM_017021307.2 | 75.8% | 75.4% | (many diffs) |
| 32 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | NM_022801.4 | 89.9% | 96.9% | (many diffs) |
| 33 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | NM_021516.4 | 88.1% | 95% | (many diffs) |
| 34 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515518.3 | 87.8% | 94.7% | (many diffs) |
| 35 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515515.3 | 87% | 93.8% | (many diffs) |
| 36 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515517.3 | 85% | 91.7% | (many diffs) |
| 37 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515514.3 | 83.8% | 90.4% | (many diffs) |
| 38 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515512.3 | 82.2% | 88.7% | (many diffs) |
| 39 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515511.3 | 81.3% | 87.7% | (many diffs) |
| 40 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515513.3 | 79.4% | 85.7% | (many diffs) |
| 41 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515519.1 | 64.1% | 69.4% | (many diffs) |
| 42 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_006515522.3 | 52.4% | 56.5% | (many diffs) |
| 43 | mouse | 17169 | Mark3 | MAP/microtubule affinity re... | XM_011244006.1 | 40.9% | 37.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2253
- ORF length:
- 2187
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cactaggacc ccattgccaa cggtgaatga acgagacact gaaaaccaca 121 cgtcacatgg agatgggcgt caagaagtta cctctcgtac cagccgctca ggagctcggt 181 gtagaaactc tatagcctcc tgtgcagatg aacaacctca catcggaaac tacagactgt 241 tgaaaacaat cggcaagggg aattttgcaa aagtaaaatt ggcaagacat atccttacag 301 gcagagaggt tgcaataaaa ataattgaca aaactcagtt gaatccaaca agtctacaaa 361 agctcttcag agaagtaaga ataatgaaga ttttaaatca tcccaatata gtgaagttat 421 tcgaagtcat tgaaactgaa aaaacactct acctaatcat ggaatatgca agtggaggtg 481 aagtatttga ctatttggtt gcacatggca ggatgaagga aaaagaagca agatctaaat 541 ttagacagat tgtgtctgca gttcaatact gccatcagaa acggatcgta catcgagacc 601 tcaaggctga aaatctattg ttagatgccg atatgaacat taaaatagca gatttcggtt 661 ttagcaatga atttactgtt ggcggtaaac tcgacacgtt ttgtggcagt cctccatacg 721 cagcacctga gctcttccag ggcaagaaat atgacgggcc agaagtggat gtgtggagtc 781 tgggggtcat tttatacaca ctagtcagtg gctcacttcc ctttgatggg caaaacctaa 841 aggaactgag agagagagta ttaagaggga aatacagaat tcccttctac atgtctacag 901 actgtgaaaa ccttctcaaa cgtttcctgg tgctaaatcc aattaaacgc ggcactctag 961 agcaaatcat gaaggacagg tggatcaatg cagggcatga agaagatgaa ctcaaaccat 1021 ttgttgaacc agagctagac atctcagacc aaaaaagaat agatattatg gtgggaatgg 1081 gatattcaca agaagaaatt caagaatctc ttagtaagat gaaatacgat gaaatcacag 1141 ctacatattt gttattgggg agaaaatctt cagagctgga tgctagtgat tccagttcta 1201 gcagcaatct ttcacttgct aaggttaggc cgagcagtga tctcaacaac agtactggcc 1261 agtctcctca ccacaaagtg cagagaagtg tttcttcaag ccaaaagcaa agacgctaca 1321 gtgaccatgc tggaccagct attccttctg ttgtggcgta tccgaaaagg agtcagacca 1381 gcactgcaga tagtgacctc aaagaagatg gaatttcctc ccggaaatca agtggcagtg 1441 ctgttggagg aaagggaatt gctccagcca gtcccatgct tgggaatgca agtaatccta 1501 ataaggcgga tattcctgaa cgcaagaaaa gctccactgt ccctagtagt aacacagcat 1561 ctggtggaat gacacgacga aatacttatg tttgcagtga gagaactaca gctgatagac 1621 actcagtgat tcagaatggc aaagaaaaca gcactattcc tgatcagaga actccagttg 1681 cttcaacaca cagtatcagt agtgcagcca ccccagatcg aatccgcttc ccaagaggca 1741 ctgccagtcg tagcactttc cacggccagc cccgggaacg gcgaaccgca acatataatg 1801 gccctcctgc ctctcccagc ctgtcccatg aagccacacc attgtcccag actcgaagcc 1861 gaggctccac taatctcttt agtaaattaa cttcaaaact cacaaggagt cgcaatgtat 1921 ctgctgagca aaaagatgaa aacaaagaag caaagcctcg atcccTACGC TTCACCTGGA 1981 GCATGAAAAC CACTAGTTCA ATGGATCCCG GGGACATGAT GCGGGAAATC CGCAAAGTGT 2041 TGGACGCCAA TAACTGCGAC TATGAGCAGA GGGAGCGCTT CTTGCTCTTC TGCGTCCACG 2101 GAGATGGGCA CGCGGAGAAC CTCGTGCAGT GGGAAATGGA AGTGTGCAAG CTGCCAAGAC 2161 TGTCTCTGAA CGGGGTCCGG TTTAAGCGGA TATCGGGGAC ATCCATAGCC TTCAAAAATA 2221 TTGCTTCCAA AATTGCCAAT GAGCTAAAGC TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 2281 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2341 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 2401 CCATTCAAAA TAGAAACCTA GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2461 ttgtgaaaga tt