Construct: ORF TRCN0000479406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014806.1_s317c1
- Derived from:
- ccsbBroadEn_11359
- DNA Barcode:
- GGCAGAATCTGCAACGCAGCTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE4A (9354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9354 | UBE4A | ubiquitination factor E4A | NM_001204077.2 | 100% | 100% | |
| 2 | human | 9354 | UBE4A | ubiquitination factor E4A | NM_004788.4 | 99.3% | 99.2% | 722_742del |
| 3 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_006510003.3 | 92.2% | 97.3% | (many diffs) |
| 4 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_011242396.2 | 92.2% | 97.3% | (many diffs) |
| 5 | mouse | 140630 | Ube4a | ubiquitination factor E4A | NM_001346698.1 | 90.6% | 95.6% | (many diffs) |
| 6 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_006510002.3 | 90.6% | 95.6% | (many diffs) |
| 7 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_011242394.2 | 90.6% | 95.6% | (many diffs) |
| 8 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_011242395.2 | 90.6% | 95.6% | (many diffs) |
| 9 | mouse | 140630 | Ube4a | ubiquitination factor E4A | NM_145400.3 | 88.8% | 93.8% | (many diffs) |
| 10 | mouse | 140630 | Ube4a | ubiquitination factor E4A | XM_017313132.1 | 85.6% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3267
- ORF length:
- 3198
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacagaccag gagaataaca acaacatctc aagtaacccc tttgctgctc 121 tttttggctc cctggctgat gccaaacagt ttgcggcaat ccaaaaagag cagctgaagc 181 aacaatctga tgaactccca gctagcccag atgactcgga taatagcgtg tcagagagcc 241 tggatgaatt cgattactct gtggctgaga ttagccgctc attccgatca cagcaggaaa 301 tatgtgagca actcaacatc aatcacatga tccaaaggat cttccttatt actctggaca 361 acagtgatcc cagcttgaaa agcgggaatg gcatccctag ccgttgtgtg tatttggaag 421 aaatggcagt agagctagaa gatcaagact ggcttgatat gagcaatgtt gagcaggccc 481 tcttcgctcg cttattactt caagatccag gcaaccactt aattaacatg acttcttcta 541 caacgctaaa tctctctgct gatcgagatg caggagagag gcacattttt tgttaccttt 601 actcctgctt ccagagagcc aaggaagaga ttaccaaagt tccagagaac ctgctaccct 661 ttgcagtgca gtgcagaaac ctcactgtgt ccaatacccg aacagttctt ctcaccccag 721 agatctatgt tgaccaaaac atccatgagc aactggtaga tttgatgtta gaagccatcc 781 agggagccca ttttgaagat gtaactgagt ttctggaaga ggtcattgaa gccttgatat 841 tggatgagga agttagaaca tttccagaag tcatgattcc agtgtttgat attttattgg 901 gccgaataaa agatctagag ctctgtcaga tccttttgta tgcatatctg gatattcttc 961 tctatttcac taggcaaaaa gatatggcaa aggtttttgt agaatacatt cagcccaagg 1021 accctaccaa tgggcaaatg taccagaaga ccttgctggg agtaattctg agtatctcct 1081 gcttattaaa gactccgggt gttgtagaaa atcatggcta ctttttgaat ccatctcgtt 1141 ccagccccca ggagatcaaa gtacaggagg ccaacatcca tcagttcatg gctcagttcc 1201 acgaaaagat ctaccagatg ctgaagaact tactccagct ctctccagaa accaaacact 1261 gtatcttgtc ctggcttgga aactgtttgc atgcaaatgc aggccgcacc aagatttggg 1321 ccaatcagat gccagaaatc tttttccaaa tgtatgcctc agatgctttc tttctgaatc 1381 tgggtgctgc tctcctgaag ctatgccagc cattttgcaa acccagatcc tctcggctcc 1441 tcacctttaa tcccacatac tgtgccctca aggagttgaa tgatgaagaa cgaaaaatta 1501 aaaatgtaca catgagaggt ttggacaaag aaacctgttt gatcccagct gtgcaggagc 1561 cgaagtttcc acagaactac aaccttgtaa cagagaacct tgctctgaca gagtacacct 1621 tgtacttggg atttcacagg ttgcatgatc agatggtaaa aatcaaccaa aatctgcatc 1681 ggctgcaggt tgcctggcgg gatgctcagc aaagttctag ccctgctgct gacaatcttc 1741 gtgagcagtt tgaacgactg atgaccatct atctttctac caagactgcc atgacagagc 1801 cacaaatgct acaaaactgc ctaaacttgc aggtgtccat ggctgttcta ctggttcaac 1861 tggccatagg caatgagggc tcacagccaa tagagctaac ctttcctttg ccagatggct 1921 acagctcttt ggcttatgtg ccagaatttt ttgcagataa cctgggtgat tttctcattt 1981 ttctccgccg ctttgccgat gacattttgg agacatcagc agattccctg gagcatgtcc 2041 ttcactttat caccattttc actggaagca tagaaagaat gaagaatccc cacctgaggg 2101 ccaaactagc agaggtgttg gaagcagtga tgccccacct ggatcagacc ccaaatccct 2161 tggtatccag tgtgttccac cggaaacgtg tgttctgcaa ctttcagtat gcaccccaac 2221 ttgcagaggc tctaatcaag gtttttgtgg acatcgaatt tacaggagac ccccatcaat 2281 ttgaacagaa gtttaattac cgccgtccca tgtatcctat cctaagatac atgtggggga 2341 cagataccta tcgggagagc attaaggatt tggctgacta tgcctctaag aatttagaag 2401 ccatgaatcc cccacttttc ctccgctttc ttaacctgct aatgaatgat gccatcttcc 2461 ttttggatga agccatacag tatttgagca agataaagat tcagcaaatt gagaaggatc 2521 gaggtgaatg ggatagtctg actccagaag cccgccgaga aaaggaggct ggcctacaga 2581 tgtttggaca gctggcacgt ttccataaca tcatgtccaa tgaaacaatc ggtacccttg 2641 cctttctcac atcagagatc aagtcactct ttgtgcatcc cttcctggct gagcgcatca 2701 tctccatgtt gaactacttc ctgcaacacc tggttggccc caagatgggt gccttaaaag 2761 tcaaggactt cagcgaattt gacttcaaac cccagcagct tgtatcagat atctgcacta 2821 tctacttaaa tcttggggat gaggagaatt tctgtgccac tgtgcccaag gatggacgtt 2881 cctattcccc aactctcttt gcacagacag ttcgagtctt gaagaaaata aataagcctg 2941 ggaatatgat tatggctttc agcaacttGG CAGAGAGAAT CAAGTCTCTT GCAGACCTCC 3001 AACAACAGGA AGAGGAAACC TATGCAGATG CCTGTGATGA GTTCCTGGAT CCCATTATGA 3061 GCACACTGAT GTGTGACCCT GTGGTGCTGC CATCTTCCAG AGTCACTGTG GATAGATCCA 3121 CCATTGCAAG ACATTTGCTC AGTGACCAAA CAGATCCCTT TAACCGTAGT CCCCTCACCA 3181 TGGACCAGAT CCGGCCAAAC ACAGAACTAA AAGAAAAAAT CCAACGGTGG CTTGCAGAGA 3241 GGAAACAACA AAAGGAGCAA CTTGAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 3301 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3361 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCAGAAT 3421 CTGCAACGCA GCTGTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3481 aagatt