Construct: ORF TRCN0000479448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003963.1_s317c1
- Derived from:
- ccsbBroadEn_13116
- DNA Barcode:
- CCGGAATGAGGCCGAGCTCCCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC25 (126364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126364 | LRRC25 | leucine rich repeat contain... | NM_145256.3 | 32.7% | 7.8% | 1_301del;602_915del |
| 2 | human | 126364 | LRRC25 | leucine rich repeat contain... | XM_005259739.4 | 32.7% | 7.8% | 1_301del;602_915del |
| 3 | human | 126364 | LRRC25 | leucine rich repeat contain... | XR_001753602.2 | 14.4% | 1_503del;804_2079del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 390
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGGCGCTG GCCGCCCGCT GTGACCTTGA 121 CCTGCAGGCC GACTGCAACT GTGCCCTGGA GTCCTGGCAC GACATCCGCC GAGACAACTG 181 CTCTGGCCAG AAGCCTCTGC TCTGCTGGGA CACAACCAGC TCCCAGCACA ACCTCTCTGC 241 CTTCCTGGAG GTCAGCTGCG CCCCTGGCCT GGCCTCTGCA ACTATCGGGG CAGTGGTGGT 301 CAGCGGGTGC CTGCTTCTTG GACTTGCCAT CGCTGGCCCT GTGCTGGCCT GGAGACTCTG 361 GCGATGCCGA GTGGCCAGAA GCCGGGAGCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGGA 541 ATGAGGCCGA GCTCCCCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt