Construct: ORF TRCN0000479488
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011775.1_s317c1
- Derived from:
- ccsbBroadEn_07810
- DNA Barcode:
- GGGACGCAAAGGGAGCAATGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MON1B (22879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479488
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22879 | MON1B | MON1 homolog B, secretory t... | NM_014940.4 | 99.8% | 99.8% | 873A>G;1274G>A |
| 2 | human | 22879 | MON1B | MON1 homolog B, secretory t... | NM_001286639.2 | 79.9% | 79.8% | 147_148ins327;546A>G;947G>A |
| 3 | human | 22879 | MON1B | MON1 homolog B, secretory t... | NM_001286640.2 | 71.2% | 71.1% | (many diffs) |
| 4 | mouse | 270096 | Mon1b | MON1 homolog B, secretory t... | NM_001048143.2 | 83.9% | 87.3% | (many diffs) |
| 5 | mouse | 270096 | Mon1b | MON1 homolog B, secretory t... | NM_173015.3 | 83.9% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1707
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtcggagga gacactgctg ccccggcccc cgggggcgcg gaggacttgg 121 aggacacgca gttccccagt gaggaagcta gagaaggtgg aggggttcac gcggtcccgc 181 cggatcccga agacgagggc ctggaggaaa caggatccaa ggacaaggac cagccaccca 241 gcccatcacc accgccccag tcagaggccc tgtcaagcac ctctcggctc tggagtcctg 301 cagcccctga gaatagtccc acatgtagcc ctgagagtag ctctggaggc cagggcgggg 361 accccagtga tgaggagtgg cgcagccagc ggaagcatgt gtttgtgctg agtgaggctg 421 gcaagcccat ctactcgcgg tatggtagtg tggaggcgct gtcggctacc atgggtgtaa 481 tgaccgccct ggtgtccttt gtgcagagtg cgggagatgc catccgtgcc atctacgctg 541 aggaccacaa gctggtgttc ctacaacagg gcccactgtt gctcgtggcc atgtcacgga 601 cttctcagtc agcagcccag ctgcgggggg agctgctagc tgtgcacgca cagatcgtga 661 gcacacttac acgtgcaagt gtcgcccgca tcttcgcaca caagcagaac tatgacctcc 721 gccgcctgct ggctggttca gagcgcacac tggaccgact tctggacagt atggagcagg 781 acccaggagc cctgctcctg ggtgccgtgc gctgtgtgcc ccttgcccgc ccgctgcgag 841 acgcactagg tgcgctcctc cgacgttgca cagcgcctgg cctggcgctg tcagtgctgg 901 cagtaggcgg tcgacttata acagcagccc aggagcggaa tgtgctggcc gagtgccggc 961 tggacccagc tgacctgcag ttgctgctcg actgggtggg tgcaccagcc tttgcggcgg 1021 gtgaggcttg ggcacctgtg tgcctgcccc gcttcaaccc tgatggtttt ttctacgcct 1081 acgtggcccg cctggatgct atgcctgtct gcctgctgct gcttggcacc caacgtgaag 1141 ccttccatgc catggccgcc tgccggcgcc tggttgaaga tgggatgcat gcccttggtg 1201 ccatgcgtgc ccttggggag gctgccagct tctctaatgc ctcatcagcc agtgctcctg 1261 cctacagcgt gcaggctgtc ggggcgccgg gcctccggca cttcctgtat aagccgctgg 1321 acatccctga ccaccaccac caactgcccc agtttaccag cccTGAGCTA GAGGCCCCCT 1381 ACAGCAGAGA GGAGGAGCGG CAGCGGCTGT CGGACCTGTA CCACCGCCTG CATGCTCGTC 1441 TCCACAGCAC CTCCCGACCC CTGCGCCTCA TTTACCACGT GGCTGAGAAG GAGACACTAC 1501 TGGCCTGGGT GACCTCCAAA TTCGAGCTCT ATACCTGCCT CAGCCCTCTG GTGACCAAGG 1561 CAGGTGCAAT CTTGGTAGTG ACCAAACTCC TGCGCTGGGT GAAGAAAGAG GAGGACCGGC 1621 TCTTCATTCG TTACCCACCC AAGTACTCCA CACCACCAGC CACCTCTACG GACCAAGCTG 1681 CCCATAATGG CTTGTTCACT GGACTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGGACGCA 1861 AAGGGAGCAA TGAAATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt