Construct: ORF TRCN0000479535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019001.4_s317c1
- Derived from:
- ccsbBroadEn_14879
- DNA Barcode:
- CAACCCAGCCTTGGCAATTCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZAP70 (7535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_001079.3 | 100% | 100% | |
| 2 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004867.1 | 83.4% | 83.4% | 1_369del |
| 3 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004868.1 | 82.6% | 82.6% | 1_369del;1254_1255insCCAGCACGCATAACGTCC |
| 4 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004869.1 | 79.3% | 78.5% | (many diffs) |
| 5 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004870.1 | 78.6% | 77.8% | (many diffs) |
| 6 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_207519.1 | 50.4% | 50.4% | 0_1ins921 |
| 7 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738927.1 | 49.5% | 1_1608del;3345_3553del;3639_3640ins35 | |
| 8 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738926.1 | 42.2% | (many diffs) | |
| 9 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738925.1 | 37.6% | (many diffs) | |
| 10 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289765.1 | 87.6% | 93.5% | (many diffs) |
| 11 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_009539.3 | 87.6% | 93.5% | (many diffs) |
| 12 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | XM_006495896.2 | 87.6% | 93.5% | (many diffs) |
| 13 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289766.1 | 84.3% | 90% | (many diffs) |
| 14 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289612.1 | 44% | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1926
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccagacccc gcggcgcacc tgcccttctt ctacggcagc atctcgcgtg 121 ccgaggccga ggagcacctg aagctggcgg gcatggcgga cgggctcttc ctgctgcgcc 181 agtgcctgcg ctcgctgggc ggctatgtgc tgtcgctcgt gcacgatgtg cgcttccacc 241 actttcccat cgagcgccag ctcaacggca cctacgccat tgccggcggc aaagcgcact 301 gtggaccggc agagctctgc gagttctact cgcgcgaccc cgacgggctg ccctgcaacc 361 tgcgcaagcc gtgcaaccgg ccgtcgggcc tcgagccgca gccgggggtc ttcgactgcc 421 tgcgagacgc catggtgcgt gactacgtgc gccagacgtg gaagctggag ggcgaggccc 481 tggagcaggc catcatcagc caggccccgc aggtggagaa gctcattgct acgacggccc 541 acgagcggat gccctggtac cacagcagcc tgacgcgtga ggaggccgag cgcaaacttt 601 actctggggc gcagaccgac ggcaagttcc tgctgaggcc gcggaaggag cagggcacat 661 acgccctgtc cctcatctat gggaagacgg tgtaccacta cctcatcagc caagacaagg 721 cgggcaagta ctgcattccc gagggcacca agtttgacac gctctggcag ctggtggagt 781 atctgaagct gaaggcggac gggctcatct actgcctgaa ggaggcctgc cccaacagca 841 gtgccagcaa cgcctcaggg gctgctgctc ccacactccc agcccaccca tccacgttga 901 ctcatcctca gagacgaatc gacaccctca actcagatgg atacacccct gagccagcac 961 gcataacgtc cccagacaaa ccgcggccga tgcccatgga cacgagcgtg tatgagagcc 1021 cctacagcga cccagaggag ctcaaggaca agaagctctt cctgaagcgc gataacctcc 1081 tcatagctga cattgaactt ggctgcggca actttggctc agtgcgccag ggcgtgtacc 1141 gcatgcgcaa gaagcagatc gacgtggcca tcaaggtgct gaagcagggc acggagaagg 1201 cagacacgga agagatgatg cgcgaggcgc agatcatgca ccagctggac aacccctaca 1261 tcgtgcggct cattggcgtc tgccaggccg aggccctcat gctggtcatg gagatggctg 1321 ggggcgggcc gctgcacaag ttcctggtcg gcaagaggga ggagatccct gtgagcaatg 1381 tggccgagct gctgcaccag gtgtccatgg ggatgaagta cctggaggag aagaactttg 1441 tgcaccgtga cctggcggcc cgcaacgtcc tgctggttaa ccggcactac gccaagatca 1501 gcgactttgg cctctccaaa gcactgggtg ccgacgacag ctactacact gcccgctcag 1561 cagggaagtg gccgctcaag tggtacgcac ccgaatgcat caacttccgc aagttctcca 1621 gccgcagcga tgtctggagc tatggggtca ccatgtggga ggccttgtcc TACGGCCAGA 1681 AGCCCTACAA GAAGATGAAA GGGCCGGAGG TCATGGCCTT CATCGAGCAG GGCAAGCGGA 1741 TGGAGTGCCC ACCAGAGTGT CCACCCGAAC TGTACGCACT CATGAGTGAC TGCTGGATCT 1801 ACAAGTGGGA GGATCGCCCC GACTTCCTGA CCGTGGAGCA GCGCATGCGA GCCTGTTACT 1861 ACAGCCTGGC CAGCAAGGTG GAAGGGCCCC CAGGCAGCAC ACAGAAGGCT GAGGCTGCCT 1921 GTGCCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGACA ACCCAGCCTT GGCAATTCAC AAACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt