Construct: ORF TRCN0000479538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012597.1_s317c1
- Derived from:
- ccsbBroadEn_04411
- DNA Barcode:
- CCGTAGCTAACTGCAGGACGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM126A (84668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479538
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84668 | FAM126A | family with sequence simila... | NM_032581.4 | 100% | 100% | |
| 2 | human | 84668 | FAM126A | family with sequence simila... | XM_011515589.2 | 100% | 100% | |
| 3 | human | 84668 | FAM126A | family with sequence simila... | NM_001363466.2 | 72.3% | 66.6% | (many diffs) |
| 4 | human | 84668 | FAM126A | family with sequence simila... | NM_001363467.2 | 71.7% | 65.8% | (many diffs) |
| 5 | human | 84668 | FAM126A | family with sequence simila... | XM_011515590.2 | 66.6% | 64.6% | (many diffs) |
| 6 | mouse | 84652 | Fam126a | family with sequence simila... | NM_053090.3 | 88.4% | 91.7% | (many diffs) |
| 7 | mouse | 84652 | Fam126a | family with sequence simila... | XM_011249798.2 | 88.4% | 91.7% | (many diffs) |
| 8 | mouse | 84652 | Fam126a | family with sequence simila... | NM_001310471.1 | 62.1% | 62.5% | (many diffs) |
| 9 | mouse | 84652 | Fam126a | family with sequence simila... | XM_017321188.1 | 62.1% | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tacttcagag aaaggggttg tggaggaatg gttgtcagag tttaagacat 121 taccagaaac atctttacca aattatgcca caaatttgaa agacaagagt tctttagttt 181 catctctcta taaagttatc caggagccac aaagtgagtt gctagaacct gtctgtcacc 241 agctctttga attctatcgc agtggagagg agcagttgct tcaatttacg ctgcaatttc 301 tcccagaact aatttggtgc taccttgcag tctcagccag cagaaatgtg catagcagtg 361 gatgcattga agctcttctt ctaggggttt acaatttgga aatagttgac aaacagggac 421 ataccaaagt attgagtttt acgattccat ctttatccaa accatctgta taccatgaac 481 cttccagcat tgggtccatg gctctgactg agagtgcact atcccagcat ggtttgtcaa 541 aagtggtata cagtggacct catcctcaaa gggagatgct gacagcacag aatcggtttg 601 aagtgttgac attccttttg ttgtgttaca atgctgcctt aacttacatg cccagtgttt 661 ctcttcagtc actgtgtcaa atttgttcaa gaatctgtgt ttgtggatat cctcgacaac 721 atgtaagaaa atataaaggt ataagtagca ggataccagt ttcttcagga ttcatggtgc 781 aaatgttaac agggatttat tttgcctttt ataatggaga atgggatcta gctcaaaaag 841 cactggatga tattatatac agagcccagc tagaattata tccagagcca ttgctggttg 901 ctaatgcaat aaaggcttca ttgcctcatg gtcccatgaa atctaataag gaaggtacaa 961 ggtgtattca agttgaaatc acaccaactt cctctcgaat atcaagaaat gcagtaacca 1021 gcatgtcaat aaggggtcat aggtggaaaa gacatggaaa tacagaatta acaggtcaag 1081 aagaactgat ggagatatct gaagttgacg agggatttta ttccagggct gcgtctagta 1141 ccagccagtc gggtttatca aacagtagtc acaattgtag taacaagcca agtataggaa 1201 agaaccacag acggtcagga ggaagcaaaa ctggaggaaa agaaaaagaa actacagggg 1261 aatcttgcaa agatcacttt gctcgaaaac aaactcagag agcccaaagt gagaatcttg 1321 agcttctctc tttgaagaga cttactttaa caaccagcca aTCCCTACCT AAGCCTAGTA 1381 GCCATGGTTT GGCTAAGACC GCAGCGACTG TATTTAGTAA ATCCTTTGAA CAAGTCAGTG 1441 GTGTCACAGT CCCACATAAC CCGTCATCTG CTGTTGGTTG TGGGGCTGGG ACAGATGCCA 1501 ATAGGTTTTC CGCTTGTAGT CTCCAAGAAG AAAAGCTTAT TTACGTTTCA GAAAGAACTG 1561 AACTTCCAAT GAAGCATCAA TCAGGTCAGC AGAGACCTCC TAGTATTAGC ATTACTCTGT 1621 CCACAGATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1681 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1741 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGTAG CTAACTGCAG GACGGTTGAC 1801 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt