Construct: ORF TRCN0000479542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006871.1_s317c1
- Derived from:
- ccsbBroadEn_14505
- DNA Barcode:
- CCCATGTGCTCCACTGGGACTGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A51P1 (442229)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92014 | SLC25A51 | solute carrier family 25 me... | NM_033412.4 | 21.8% | 20.2% | (many diffs) |
| 2 | human | 147407 | SLC25A52 | solute carrier family 25 me... | NM_001034172.4 | 21.7% | 19.8% | (many diffs) |
| 3 | human | 442229 | SLC25A51P1 | SLC25A51 pseudogene 1 | NR_026540.1 | 12.7% | 1_538del;735_736delAT;746_1605del | |
| 4 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026564.1 | 10.7% | (many diffs) | |
| 5 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026563.1 | 9.9% | (many diffs) | |
| 6 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026541.1 | 8.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 300
- ORF length:
- 207
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGATTC AGAAGCTCAT GAAAAGAGGC 121 CACCAACACT AACATCTTCA GAACAAGATA TATCACCTCG TATTACAAAT GTTGGTGAAA 181 TGAAGCATTA CTTGTGTGGC TGCTGCGCAG CCTTCAACAA CATCGCAATC ACATATCCCA 241 TTCAGACGGT CCTCTTTCGA CAACAGCTGT ATGGCATCAA AACCCGGGGC AATATTTTTG 301 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 361 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 421 TATATATCTT GTGGAAAGGA CGACCCATGT GCTCCACTGG GACTGCCACG CGTTAAGTCg 481 acaatcaacc tctggattac aaaatttgtg aaagatt