Construct: ORF TRCN0000479544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001733.1_s317c1
- Derived from:
- ccsbBroadEn_14335
- DNA Barcode:
- TGCATACGTCCCTATATTCGCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SHF (90525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90525 | SHF | Src homology 2 domain conta... | XM_017022711.2 | 74% | 23.3% | 118delG;553_554delGT;639_858del |
| 2 | human | 90525 | SHF | Src homology 2 domain conta... | NM_001301171.1 | 57.5% | 26% | (many diffs) |
| 3 | human | 90525 | SHF | Src homology 2 domain conta... | XM_006720742.3 | 51.1% | 16.2% | 1_606del;724delG |
| 4 | human | 90525 | SHF | Src homology 2 domain conta... | XM_011522175.2 | 51.1% | 16.2% | 1_606del;724delG |
| 5 | human | 90525 | SHF | Src homology 2 domain conta... | NM_138356.3 | 50% | 15.9% | (many diffs) |
| 6 | human | 90525 | SHF | Src homology 2 domain conta... | XM_011522174.2 | 49.5% | 15.7% | (many diffs) |
| 7 | human | 90525 | SHF | Src homology 2 domain conta... | NM_001301169.2 | 39.7% | 17.5% | 1_606del;724delG;846_847ins141 |
| 8 | human | 90525 | SHF | Src homology 2 domain conta... | NM_001301168.2 | 33.7% | 14.5% | (many diffs) |
| 9 | human | 90525 | SHF | Src homology 2 domain conta... | XM_017022710.1 | 31.4% | 16.7% | (many diffs) |
| 10 | human | 90525 | SHF | Src homology 2 domain conta... | XM_011522176.2 | 26.5% | 14.9% | (many diffs) |
| 11 | human | 90525 | SHF | Src homology 2 domain conta... | XM_011522178.2 | 25.1% | 15.1% | (many diffs) |
| 12 | human | 90525 | SHF | Src homology 2 domain conta... | XM_011522177.2 | 24.9% | 15.9% | (many diffs) |
| 13 | human | 90525 | SHF | Src homology 2 domain conta... | NM_001301170.2 | 20.3% | 13.1% | (many diffs) |
| 14 | mouse | 435684 | Shf | Src homology 2 domain conta... | XM_017319108.2 | 68.6% | 22% | (many diffs) |
| 15 | mouse | 435684 | Shf | Src homology 2 domain conta... | NM_001013829.2 | 52.9% | 19.1% | (many diffs) |
| 16 | mouse | 435684 | Shf | Src homology 2 domain conta... | NM_001374011.1 | 40.5% | 13.1% | (many diffs) |
| 17 | mouse | 435684 | Shf | Src homology 2 domain conta... | XM_006499877.2 | 31.2% | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 555
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccctat gaggctcaaa agatgatggc cgagatccgg ggctccaagg 121 agacagcaac tcagcccttg cctctgtatg acacacccta tgagccagag gaggatgggg 181 ccacccggaa ggtgaggggg ccccctggcc ccgggagtcc cgcctgccag aggatgatga 241 gaggccccct gaggagtatg accagccctg ggagtggaag aaggagcgga tttccaaagc 301 ctttgcagtt gacattaagg tcatcaaaga cctaccttgg cctccacctg tgggacagct 361 ggacagcagc ccctccctgc ctgatgggga cagggacatc tccggtccag cctcgccccT 421 CCCTGAGCCC AGCCTGGAGG ACAGCAGCGC CCAGTTTGAA GGACCGGAGA AGAGCTGCCT 481 GTCACCTGGC CGGGAGGAGA AGGGGCGGCT ACCTCCCCGA CTCTCTGCAG GGAACCCCAA 541 GTCAGCCAAA CCCCTAAGCA TGGAGCCCAG CAGCCCCCTG GGGGAGTGGA CAGATCCAGC 601 ACTGCCTCTG GAAAACCAGC TGGTATCACG GGGCCATCAG CCGAACCGAC GCCGAGAACC 661 TGCTCCGGCT GTGCAAAGAG GCCAGCTACC TGGTGCGCAA CAGTTGCCAA CTTTCTTGTA 721 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 781 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 841 AAAGGACGAT GCATACGTCC CTATATTCGC GTCACGCGTT AAGTCgacaa tcaacctctg 901 gattacaaaa tttgtgaaag att