Construct: ORF TRCN0000479593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004905.1_s317c1
- Derived from:
- ccsbBroadEn_04156
- DNA Barcode:
- AAACCAGCTGTTTCAAATCCATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR76 (79968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79968 | WDR76 | WD repeat domain 76 | NM_001167941.2 | 100% | 100% | |
| 2 | human | 79968 | WDR76 | WD repeat domain 76 | XM_005254680.3 | 100% | 100% | |
| 3 | human | 79968 | WDR76 | WD repeat domain 76 | NM_024908.4 | 89.7% | 89.7% | 1_192del |
| 4 | human | 79968 | WDR76 | WD repeat domain 76 | XR_001751398.2 | 44% | 1_232del;1919_3829del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1755
- ORF length:
- 1686
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgccccaaa tccctatcag aaaagaattc taacaatgaa gtggcgtgta 121 agaagactaa aataaagaaa acttgcagaa ggattatacc tccaaagatg aaaaacacat 181 cttccaaggc agaatccacg ctgcaaaatt catcctcagc tgttcatact gaaagtaaca 241 agctacaacc caagagaacg gcagatgcga tgaatctcag tgttgatgtg gaaagtagtc 301 aggatggaga cagtgatgaa gataccacac catccctgga tttttcggga ttgtcaccct 361 acgaaaggaa gagactgaag aacatatcag aaaacgcaga cttttttgct tctcttcagt 421 tgtctgagtc tgctgcaaga ctccgtgaaa tgatagagaa gagacagcct cctaaatcca 481 aaagaaagaa gcctaagaga gaaaatggga ttggatgtag aaggtcaatg cgattactaa 541 aagttgatcc ttcgggagtt tcattaccag cagctccaac accgccgaca ttagtagcag 601 atgaaactcc tttgttacct cctgggcctt tagaaatgac ttctgaaaat caagaagaca 661 acaatgaacg atttaaagga tttctgcaca catgggcagg aatgagcaag ccaagtagta 721 agaacactga gaagggatta tctagcatta aaagctacaa agccaattta aatggcatgg 781 tcattagtga agataccgtt tacaaagtta ccacaggccc aatattctct atggctctcc 841 atccatcaga aactagaact ttggtagcag ttggggccaa atttgggcaa gttggacttt 901 gtgatttgac ccagcaacct aaagaagatg gagtttatgt ttttcatccc catagtcagc 961 cagttagctg tctttacttc tcacccgcca atccggccca catactgtca ctgagctatg 1021 atggcacgtt acgctgtggg gatttttcca gggctatttt tgaagaggtg tatagaaatg 1081 aaagaagtag cttttcctcc ttcgacttct tggcagaaga tgcctccact ttaatagtag 1141 gacactggga tggaaatatg tcactggtgg atagacggac acctggaact tcttatgaga 1201 aacttaccag ttcttctatg ggaaaaataa gaactgttca tgtccaccca gtgcatagac 1261 agtattttat cactgccgga ttgagggata ctcatattta tgatgcaagg cgattgaatt 1321 ccaggagaag tcagcctttg atttctttga ctgaacatac aaagagcatt gcttccgcct 1381 atttttcacc tcttactggt aacagagtgg tgacCACATG TGCTGATTGT AATCTGAGAA 1441 TTTTTGACAG CAGCTGTATA TCTTCTAAGA TTCCGCTCCT CACCACCATC AGGCACAACA 1501 CTTTCACTGG GCGATGGCTG ACCAGGTTCC AAGCCATGTG GGATCCTAAA CAAGAAGACT 1561 GTGTCATAGT TGGCAGCATG GCCCATCCAC GACGGGTAGA AATCTTCCAT GAGACAGGAA 1621 AGAGGGTGCA TTCGTTTGGT GGAGAATACC TTGTCTCTGT GTGTTCCATC AATGCCATGC 1681 ACCCAACTCG GTATATTTTG GCTGGAGGTA ATTCCAGCGG GAAGATACAT GTTTTTATGA 1741 ATGAAAAAAG CTGCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAACCAGCTG TTTCAAATCC 1921 ATGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt