Construct: ORF TRCN0000479602
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015342.1_s317c1
- Derived from:
- ccsbBroadEn_08014
- DNA Barcode:
- CCTATTCGAATTCCTTACTCGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBGT1 (26301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479602
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26301 | GBGT1 | globoside alpha-1,3-N-acety... | NM_021996.6 | 99.8% | 99.7% | 58C>T;987C>T |
| 2 | human | 26301 | GBGT1 | globoside alpha-1,3-N-acety... | NM_001282632.2 | 94.9% | 94.5% | 58C>T;137_138ins51;936C>T |
| 3 | human | 26301 | GBGT1 | globoside alpha-1,3-N-acety... | NM_001288572.2 | 86.3% | 86.4% | 0_1ins141;846C>T |
| 4 | human | 26301 | GBGT1 | globoside alpha-1,3-N-acety... | NM_001282629.2 | 84.6% | 38.2% | 58C>T;359_360insGTACACTCATTTCATCCAG;882_883ins140 |
| 5 | human | 26301 | GBGT1 | globoside alpha-1,3-N-acety... | NM_001288573.2 | 60.7% | 60.8% | 0_1ins408;579C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1107
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcgccggaga ctggccctgg gtctggggtt ctgcctgttg gcgggcacaa 121 gcttcagtgt cctgtgggtg tatcttgaga actggctgcc agtctcctat gtcccctatt 181 atctcccctg cccagagatc ttcaacatga agctgcacta caagagggag aagccactcc 241 agcccgtggt atggtcacag taccctcagc ccaagctgct ggagcacagg cccacacagc 301 tgctgacact cacaccctgg ttggcgccca tcgtctccga gggaaccttc aacccagagc 361 ttctgcagca catctaccag ccactgaacc tgaccattgg ggtcacggtg tttgccgtgg 421 ggaagtacac tcatttcatc cagtccttcc tggagtcagc cgaggagttc ttcatgcgtg 481 ggtaccgggt gcactactac atcttcactg acaaccctgc agccgttccc ggggtcccgc 541 tgggtcccca ccggcttctc agctccatcc ccatccaggg tcactcccac tgggaggaga 601 catccatgcg ccggatggag accatcagcc agcacattgc taagagggct caccgggagg 661 tggactacct cttctgcctt gatgtggaca tggtgtttcg gaacccgtgg ggccctgaga 721 ccttgggaga cctggtggct gccattcacc caagctacTA CGCCGTTCCC CGCCAGCAGT 781 TCCCCTATGA GCGCAGGCGT GTTTCCACTG CCTTTGTGGC AGACAGCGAA GGGGACTTCT 841 ATTATGGTGG GGCAGTCTTC GGGGGGCAGG TGGCCAGGGT ATATGAGTTT ACTAGGGGCT 901 GCCACATGGC CATCCTGGCG GACAAGGCCA ATGGCATCAT GGCTGCCTGG CGGGAGGAAA 961 GCCACCTGAA CCGTCACTTC ATCTCAAACA AGCCGTCCAA GGTGCTGTCC CCCGAGTACC 1021 TCTGGGACGA CAGGAAGCCC CAGCCACCCA GTCTGAAGCT GATCCGCTTT TCTACACTGG 1081 ACAAGGATAT CAGCTGCCTG AGGAGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTATTCG 1261 AATTCCTTAC TCGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt