Construct: ORF TRCN0000479605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003002.1_s317c1
- Derived from:
- ccsbBroadEn_13875
- DNA Barcode:
- CACTTCATTTGTATTATAGTATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGFG2 (3268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479605
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012113.1 | 50.3% | 48.3% | (many diffs) |
| 2 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | NM_006076.5 | 31% | 29.9% | (many diffs) |
| 3 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_005250306.2 | 30.3% | 29.2% | (many diffs) |
| 4 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012112.1 | 13.1% | 12.2% | (many diffs) |
| 5 | mouse | 231801 | Agfg2 | ArfGAP with FG repeats 2 | XM_006504570.2 | 31.2% | 31.8% | (many diffs) |
| 6 | mouse | 231801 | Agfg2 | ArfGAP with FG repeats 2 | XM_006504569.2 | 28.4% | 29% | (many diffs) |
| 7 | mouse | 231801 | Agfg2 | ArfGAP with FG repeats 2 | NM_178162.3 | 28.4% | 29% | (many diffs) |
| 8 | mouse | 231801 | Agfg2 | ArfGAP with FG repeats 2 | NM_001303271.1 | 27.8% | 28.4% | (many diffs) |
| 9 | mouse | 231801 | Agfg2 | ArfGAP with FG repeats 2 | NM_001303266.1 | 27.7% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gatggcggcg aagaagggcc cgggcccggg cggcggggtc agcgggggca 121 aggcggaggc ggaggcggcc TCGGAGGTGT GGTGCCGTCG GGTGCGGGAG CTGGGTGGCT 181 GCAGCCAGGC CGGGAACCGC CACTGCTTCG AGTGCGCCCA GCGCGGGGTC ACCTACGTGG 241 ATATCACCGT GGGCAGCTTC GTGTGCACCA CCTGCTCCGG CCTCCTGAGA GGGCTGAACC 301 CCCCTCATCG TGTCAAGTCA ATCTCCATGA CAACTTTCAC TGAGCCTGAA GTAGTATTCC 361 TGCAATCCCG TGGAAATGAG GTTTGCCGGA AGATTTGGTT GGGTCTGTTT GATGCTCGGA 421 CATCTTTAGT ACCAGATTCC AGGGATCCTC AGAAAGTGAA GGAGTTTCTC CAGGAAAAAT 481 ATGAGAAGAA GAGATGGCCA GACACCTTCC CAAGGAGGCT TTGCCAACTT TGCCCAGCTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACACT TCATTTGTAT TATAGTATAT ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt