Construct: ORF TRCN0000479632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006471.1_s317c1
- Derived from:
- ccsbBroadEn_07691
- DNA Barcode:
- TGCTTTCCACCTATATGGGCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R13L (10848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10848 | PPP1R13L | protein phosphatase 1 regul... | NM_001142502.2 | 99.9% | 99.8% | 592C>T |
| 2 | human | 10848 | PPP1R13L | protein phosphatase 1 regul... | NM_006663.4 | 99.9% | 99.8% | 592C>T |
| 3 | human | 10848 | PPP1R13L | protein phosphatase 1 regul... | XM_017026177.1 | 99.9% | 99.8% | 592C>T |
| 4 | human | 10848 | PPP1R13L | protein phosphatase 1 regul... | XM_017026178.1 | 99.9% | 99.8% | 592C>T |
| 5 | human | 10848 | PPP1R13L | protein phosphatase 1 regul... | XM_017026179.1 | 73.5% | 73.3% | (many diffs) |
| 6 | mouse | 333654 | Ppp1r13l | protein phosphatase 1, regu... | NM_001010836.3 | 82.3% | 88.1% | (many diffs) |
| 7 | mouse | 333654 | Ppp1r13l | protein phosphatase 1, regu... | XM_006540126.1 | 82.3% | 88.1% | (many diffs) |
| 8 | mouse | 333654 | Ppp1r13l | protein phosphatase 1, regu... | XM_006540127.3 | 82.3% | 88.1% | (many diffs) |
| 9 | mouse | 333654 | Ppp1r13l | protein phosphatase 1, regu... | XM_006540128.2 | 82.3% | 88.1% | (many diffs) |
| 10 | mouse | 333654 | Ppp1r13l | protein phosphatase 1, regu... | XM_006540129.1 | 82.3% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2550
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cagcgaggca ttccagagcg cgcgggactt tctggacatg aacttccagt 121 cgctggccat gaaacacatg gatctgaagc agatggagct ggacacggcg gcggccaagg 181 tggatgaact gaccaagcag ctggagtcgc tgtggtcaga ctctcccgcg cctcctggcc 241 cgcaggccgg acccccttct aggccgcccc ggtacagctc cagctcgatc cctgagccct 301 tcggcagccg agggtccccc cggaaggcgg ccaccgacgg cgcagacacc ccgttcggac 361 gatcagagag tgccccaacc ctacacccct acagcccgct gtcccccaag ggacggccgt 421 cgtcgccgcg caccccgctc tacctgcagc cggacgccta cggcagcctg gaccgcgcga 481 cctcgccccg gccccgcgcc ttcgatggcg caggcagctc cctcggccgt gcgccctccc 541 cgcggcccgg gccaggcccg ctccgccagc agggtccccc cacgcctttc gacttcctgg 601 gccgcgcagg ctccccccgc ggcagccccc tggcggaggg gccccaggcc ttcttctccg 661 agcgtgggcc gtcaccgcgc ccccctgcca cagcctacga cgcgccagcg tccgccttcg 721 ggagctccct gctaggctcc ggcggcagcg cattcgcccc gcctctgcgc gcgcaagacg 781 acctgacgct gcgccggcgg cctccgaaag cctggaacga gtctgacctg gacgtggcgt 841 acgagaagaa gccttcgcag acagcgagct atgaacgcct ggacgtcttc gcaaggcctg 901 cctcgccgag cctgcagctg ttgccttgga gggagagcag cctggatgga ctggggggca 961 ccggcaagga caacctcact agcgccaccc tgccgcgcaa ttacaaggtc tctcctctgg 1021 ccagcgaccg gcgttcagac gcgggcagct accggcgctc gctgggctcc gcggggccgt 1081 cgggcacttt gcctcgcagc tggcagcccg tcagccgcat ccccatgccc ccctccagcc 1141 cccagccccg cggggccccg cgccagcgtc ccatccccct cagcatgatc ttcaagctgc 1201 agaacgcctt ctgggagcac ggggccagcc gcgccatgct ccctgggtcc cccctcttca 1261 cccgagcacc cccgcctaag ctgcagcccc aaccacaacc acagccccag ccacaatcac 1321 aaccacagcc ccagctgccc ccacagcccc agacccaacc ccaaacccct accccagccc 1381 cccagcatcc ccaacagaca tggccccctg tgaacgaagg accccccaaa ccccccaccg 1441 agctggagcc tgagccggag atagaggggc tgctgacacc agtgctggag gctggcgatg 1501 tggatgaagg ccctgtagca aggcctctca gccccacgag gctgcagcca gcactgccac 1561 cggaggcaca gtcggtgccc gagctggagg aggtggcacg ggtgttggcg gaaattcccc 1621 ggcccctcaa acgcaggggc tccatggagc aggcccctgc tgtggccctg ccccctaccc 1681 acaagaaaca gtaccagcag atcatcagcc gcctcttcca tcgtcatggg gggccagggc 1741 ccggggggcc ggagccagag ctgtccccca tcactgaggg atctgaggcc agggcagggc 1801 cccctgctcc tgccccacca gctcccattc cacccccggc cccgtcccag agcagcccac 1861 cagagcagcc gcagagcatg gagatgcgct ctgtgctgcg gaaggcgggc tccccgcgca 1921 aggcccgccg cgcgcgcctc aaccctctgg tgctcctcct ggacgcggcg ctgaccgggg 1981 agctggaggt ggtgcagcag gcggtgaagg agatgaacga cccgagccag cccaacgagg 2041 agggcatcac tgccttgcac aacgccatct gcggcgccaa ctactctatc gtggatttcc 2101 tcatcaccgc gggtgccaat gtcaactccc ccgacagcca cggctggaca cccttgcact 2161 gcgcggcgtc gtgcaacgac acagtcatct gcatggcgct ggtgcagcac ggcgctgcaa 2221 tcttcgccac cacgctcagc gacggcgcca ccgccTTCGA GAAGTGCGAC CCTTACCGCG 2281 AGGGTTATGC TGACTGCGCC ACCTACCTGG CAGACGTCGA GCAGAGTATG GGGCTGATGA 2341 ACAGCGGGGC AGTGTACGCT CTCTGGGACT ACAGCGCCGA GTTCGGGGAC GAGCTGTCCT 2401 TCCGCGAGGG CGAGTCGGTC ACCGTGCTGC GGAGGGACGG GCCGGAGGAG ACCGACTGGT 2461 GGTGGGCCGC GCTGCACGGC CAGGAGGGCT ACGTGCCGCG GAACTACTTC GGGCTGTTCC 2521 CCAGGGTGAA GCCTCAAAGG AGTAAAGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2581 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2641 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCTT 2701 TCCACCTATA TGGGCGGCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2761 tgaaagatt