Construct: ORF TRCN0000479647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005518.1_s317c1
- Derived from:
- ccsbBroadEn_05114
- DNA Barcode:
- GAGGACTTATGCGCACCTGCCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXD1 (161829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | NM_152596.4 | 99.8% | 100% | 1194A>G;1209C>T |
| 2 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_011521299.2 | 97.8% | 97.8% | (many diffs) |
| 3 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_011521301.2 | 95.5% | 95.7% | 26_94del;1263A>G;1278C>T |
| 4 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_006720415.3 | 93.1% | 92.1% | (many diffs) |
| 5 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_011521300.2 | 92.8% | 92.9% | 0_1ins108;1086A>G;1101C>T |
| 6 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_017021970.1 | 92.8% | 92.9% | 0_1ins108;1086A>G;1101C>T |
| 7 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_017021971.1 | 92.8% | 92.9% | 0_1ins108;1086A>G;1101C>T |
| 8 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | NM_001286441.2 | 89.3% | 88.2% | (many diffs) |
| 9 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_011521298.2 | 89.3% | 88.2% | (many diffs) |
| 10 | human | 161829 | EXD1 | exonuclease 3'-5' domain co... | XM_011521302.2 | 71.6% | 71.7% | 0_1ins435;759A>G;774C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggacagtgaa ttcctagctt atgtggaact actagatgaa gtggaacaag 121 gctcagtgag agcaaaagca tcttctgtta gtctacatgc agaaagaacc tggatggaga 181 aaatgaaagt tgaagaccta aatgtatgtg agcctgcttc tcctgcccct gaagcaccag 241 ctacctctct gctgaatgac ctcaagtaca gcccatcaga ggaagaggag gtgacataca 301 cagtcattaa tcaattccag cagaagtttg gtgctgcgat actccatatc aagaagcaga 361 atgtcctgag tgtggcagca gaaggagcga atgtatgtcg ccatggcaaa ctgtgctggc 421 tgcaggtggc cacaaattgc cgagtttact tatttgacat tttccttctg ggaagtcgag 481 ctttccacaa tggacttcag atgatactag aagacaagag aattttgaag gttatccatg 541 attgtcgttg gctttctgat tgcctctctc atcagtatgg aattttgctg aataatgtct 601 ttgacacaca ggtagcagat gtacttcagt tttccatgga aacgggtggc tatcttccaa 661 actgcatcac tactttgcag gagagtttaa tcaaacacct tcaagtagcc cctaaatatc 721 tctcctttct agaaaagaga caaaaactaa ttcaggaaaa tccagaagta tggttcatcc 781 gacctgtttc accctcttta ctgaaaattt tggccctgga agctacctac ctgttacccc 841 ttcgcttggc actcctagat gagatgatgt ctgacctaac caccctggtg gatggttacc 901 taaacacgta tcgcgaaggg tctgcagacc ggcttggagg cactgagcct acatgtatgg 961 agctgccaga ggaactgctt caactcaagg acttccagaa gcagcgcagg gagaaagctg 1021 caagagaata tagggtgaat gcacagggac tcctgataag gacagtgcta cagccaaaga 1081 aattagtgac agagacagca gggaaagagg agaaagtcaa aggcttctta tttggtaaaa 1141 attttaggat agataaagct ccaagtttta catctcaaga ctttcacggg gatgtgaatt 1201 tactgaaaga agaatctttg aataaacaag ctacaaatcc tcaacatcta ccTCCCACGG 1261 AGGAAGGGGA AACTAGTGAG GATTCCAGTA ACAAACTCAT TTGCACAAAG TCAAAGGGGT 1321 CAGAGGACCA GAGAATAACT CAGAAAGAAC ACTTTATGAC ACCCAAACAT GAGTTTCAGG 1381 CAAGTTTATC TTTGAAAGAG GAGACAGAAC AGTTATTGAT GGTGGAAAAC AAGGAAGATT 1441 TAAAATGCAC AAAACAGGCT GTTTCAATGT CTTCCTTTCC TCAGGAAACC AGAGTGTCTC 1501 CAAGTGACAC TTTTTATCCT ATCAGAAAGA CTGTGGTTTC CACACTCCCT CCCTGTCCAG 1561 CCTTGGAGAA GATCGATTCC TGGATAAGTC CTTTTCTAAA TCTGCCCTAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAGAGGACT TATGCGCACC TGCCCGGACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt