Transcript: Human NM_152596.4

Homo sapiens exonuclease 3'-5' domain containing 1 (EXD1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
EXD1 (161829)
Length:
2999
CDS:
252..1796

Additional Resources:

NCBI RefSeq record:
NM_152596.4
NBCI Gene record:
EXD1 (161829)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152596.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322718 GGCCACAAATTGCCGAGTTTA pLKO_005 614 CDS 100% 13.200 18.480 N EXD1 n/a
2 TRCN0000322776 TGGTGCTGCGATACTCCATAT pLKO_005 515 CDS 100% 10.800 15.120 N EXD1 n/a
3 TRCN0000051402 GAACACTTTATGACACCCAAA pLKO.1 1533 CDS 100% 4.050 5.670 N EXD1 n/a
4 TRCN0000322719 CCTGGTGGATGGTTACCTAAA pLKO_005 1070 CDS 100% 10.800 8.640 N EXD1 n/a
5 TRCN0000322720 ATGAAAGTTGAAGACCTAAAT pLKO_005 369 CDS 100% 13.200 9.240 N EXD1 n/a
6 TRCN0000322777 CATGTTGTAAATGTAGTAATG pLKO_005 1877 3UTR 100% 10.800 7.560 N EXD1 n/a
7 TRCN0000162795 CTTCCAAAGTGCTGGGATTAT pLKO.1 2677 3UTR 100% 13.200 6.600 Y SLC48A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152596.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05114 pDONR223 100% 99.8% 100% None 1194A>G;1209C>T n/a
2 ccsbBroad304_05114 pLX_304 0% 99.8% 100% V5 1194A>G;1209C>T n/a
3 TRCN0000479647 GAGGACTTATGCGCACCTGCCCGG pLX_317 23.4% 99.8% 100% V5 1194A>G;1209C>T n/a
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