Construct: ORF TRCN0000479648

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005475.2_s317c1
Derived from:
ccsbBroadEn_14249
DNA Barcode:
GACATCTCACACGCCATAGCCTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NT5DC2 (64943)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479648
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64943 NT5DC2 5'-nucleotidase domain cont... NM_022908.3 88% 87.5% (many diffs)
2 human 64943 NT5DC2 5'-nucleotidase domain cont... XM_005265406.2 84.3% 84.5% (many diffs)
3 human 64943 NT5DC2 5'-nucleotidase domain cont... XM_006713305.2 84.3% 84.5% (many diffs)
4 human 64943 NT5DC2 5'-nucleotidase domain cont... NM_001134231.2 82.5% 82.5% (many diffs)
5 human 64943 NT5DC2 5'-nucleotidase domain cont... XM_024453719.1 79.7% 79.9% (many diffs)
6 human 64943 NT5DC2 5'-nucleotidase domain cont... XM_006713303.3 75.3% 75.4% (many diffs)
7 mouse 70021 Nt5dc2 5'-nucleotidase domain cont... NM_027289.1 76.1% 82.8% (many diffs)
8 mouse 70021 Nt5dc2 5'-nucleotidase domain cont... XM_006519507.2 74.8% 81.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1449
ORF length:
1383
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg gagactggtg cacgacctcc tgccccccga ggtctgcagt ctcctgaacc
121cagcagccat ctacgccaac aacgagatca gcctgcgtga cgttgaggtc tacggctttg
181actacgacta caccctggac cagtatgcag acgcactgca ccccgagatc ttcagtaccg
241cccgtgacat cctgatcgag cactacaaga gccttctgat gaagattgac gccttccact
301acgtgcagct ggggacagcc tacaggggcc tccagcctgt gccagacgag gaggtgattg
361agctgtatgg gggtacccag cacatcccac tataccagat gagtggcttc tatggcaagg
421gtccctccat taagcagttc atggacatct tctcgctacc ggagatggct ctgctgtcct
481gtgtggtgga ctactttctg ggccacagcc tggagtttga ccaagcacat ctctacaagg
541acgtgacgga cgccatccga gacgtgcatg tgaagggcct catgtaccag tggatcgagc
601aggacatgga gaagtacatc ctgagagggg atgagacgtt tgctgtcctg agccgcctgg
661tggcccatgg gaagcagctg ttcctcatca ccaacagtcc tttcagcttc gtagacaagg
721ggatgcggca catggtgggt cccgattggc gccagctctt cgatgtggtc attgtccagg
781cagacaagcc cagcttcttc actgaccggc gcaagccttt cagaaaactc gatgagaagg
841gctcacttca gtgggaccgg atcacccgct tggaaaaggg caagatctat cggcagggaa
901acctgtttga cttcttgcgc ttgacggaat ggcgtggccc ccgcgtgctc tacttcgggg
961accacctcta tagtgatctg gcggatctca tgctgcggca cggctggcgc acaggcgcca
1021tcatccccga gctggagcgt gagatccgca tcatcaacac ggagcagtac atgcactcgc
1081tgacgtggca acaggcgctc acggggctgc tggagcgcat gcagacctat caggacgcgg
1141agtcgaggca ggtgctggct gcctggatga aagagcggca ggagctgagg tgcaTCACCA
1201AGGCCCTGTT CAATGCGCAG TTCGGCAGCA TCTTCCGCAC CTTCCACAAC CCCACCTACT
1261TCTCAAGGCG CCTCGTGCGC TTCTCTGACC TCTACATGGC CTCCCTCAGC TGCCTGCTCA
1321ACTACCGCGT GGACTTCACC TTCTACCCAC GCCGTACGCC GCTGCAGCAC GAGGCACCCC
1381TCTGGATGGA CCAGCTCTGC ACCGGCTGCA TGAAGACCCC CTTCCTTGGT GACATGGCCC
1441ACATCCGCTG CACAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1501CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1561TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACATC TCACACGCCA TAGCCTTAAC
1621GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)