Construct: ORF TRCN0000479662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003501.1_s317c1
- Derived from:
- ccsbBroadEn_12933
- DNA Barcode:
- ATGCGTACCTCTTTGCTGCATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN18 (90139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90139 | TSPAN18 | tetraspanin 18 | NM_130783.5 | 91.1% | 87.6% | (many diffs) |
| 2 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_006718373.4 | 91.1% | 87.6% | (many diffs) |
| 3 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_011520459.3 | 91.1% | 87.6% | (many diffs) |
| 4 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_005253217.3 | 87.9% | 84.5% | (many diffs) |
| 5 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_006718372.3 | 87.9% | 84.5% | (many diffs) |
| 6 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_017018531.1 | 66.9% | 63.4% | (many diffs) |
| 7 | human | 90139 | TSPAN18 | tetraspanin 18 | XM_017018530.1 | 64.5% | 61.1% | (many diffs) |
| 8 | mouse | 241556 | Tspan18 | tetraspanin 18 | NM_183180.2 | 79.8% | 79.8% | (many diffs) |
| 9 | mouse | 241556 | Tspan18 | tetraspanin 18 | XM_006499474.3 | 79.8% | 79.8% | (many diffs) |
| 10 | mouse | 241556 | Tspan18 | tetraspanin 18 | XM_006499475.3 | 79.8% | 79.8% | (many diffs) |
| 11 | mouse | 241556 | Tspan18 | tetraspanin 18 | XM_006499476.3 | 79.8% | 79.8% | (many diffs) |
| 12 | mouse | 241556 | Tspan18 | tetraspanin 18 | XM_006499477.3 | 79.8% | 79.8% | (many diffs) |
| 13 | mouse | 241556 | Tspan18 | tetraspanin 18 | XM_011239525.2 | 79.8% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 867
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggcgactgt ctgagctgca tgaagtatct gatgtttgta ttcaatttct 121 tcatatttct gggcggggcc tgcctgctgg ccatcggcat ctgggtcatg gtggacccca 181 ccggcttccg ggagatcgtg gctgccaatc ctctgctcct cacgggcgcc tacatcctcc 241 tggccatggg gggcctgctc tttctgctcg gcttcctggg ctgctgcggg gccgtccgtg 301 agaacaagtg tctgctgcta tttttcttcc tgttcatcct gatcatcttc ctggcagagc 361 tctcagcagc catcctggcc ttcatcttca gggaaaatct cacccgagaa ttcttcacca 421 aggagctcac caagcactac cagggcaata acgacacaga cgtcTTCTCT GCCACCTGGA 481 ACTCGGTCAT GATCACATTT GGTTGCTGCG GGGTCAACGG GCCTGAAGAC TTTAAGTTTG 541 CATCTGTGTT TCGACTCCTG ACCCTGGATA GTGAAGAGGT GCCGGAGGCC TGCTGCCGGA 601 GGGAACCCCA AAGTCGGGAC GGGGTCCTGC TGAGCCGGGA GGAGTGCCTC CTGGGAAGGA 661 GCCTATTCCT AAACAAGCAG GGCTGTTACA CGGTGATCCT CAACACCTTC GAGACCTACG 721 TCTACTTGGC CGGAGCCCTT GCCATCGGGG TACTGGCCAT CGAGGAAGAA AGAGCTCATT 781 CTGTCTCACA AGCCACCGGC ATCCTGTATC AGCTTCCAGC CTCCCCTCAG GCTTTCCAGT 841 CACCAGGGAC ACTCGGAGCC ACAGCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 901 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 961 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGCGTAC 1021 CTCTTTGCTG CATACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1081 aagatt