Construct: ORF TRCN0000479670

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008957.1_s317c1
Derived from:
ccsbBroadEn_00903
DNA Barcode:
CAAGAGGGCTGTCGGTTGTTCTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCNQ1 (3784)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479670
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3784 KCNQ1 potassium voltage-gated cha... NM_181798.1 100% 100%
2 human 3784 KCNQ1 potassium voltage-gated cha... NM_000218.3 81.1% 80.9% 1_362del;366_384del;386T>A
3 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_006508492.2 82% 88% (many diffs)
4 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_017321998.1 79.2% 85% (many diffs)
5 mouse 16535 Kcnq1 potassium voltage-gated cha... NM_008434.2 68.5% 73.4% (many diffs)
6 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_006508488.3 66.6% 71.3% (many diffs)
7 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_011241982.2 60.8% 60.8% (many diffs)
8 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_006508490.3 48.7% 53% (many diffs)
9 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_006508491.3 48.7% 53% (many diffs)
10 mouse 16535 Kcnq1 potassium voltage-gated cha... XM_006508489.3 47.4% 51.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1716
ORF length:
1647
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggacttcctc atcgtcctgg tctgcctcat cttcagcgtg ctgtccacca
121tcgagcagta tgccgccctg gccacgggga ctctcttctg gatggagatc gtgctggtgg
181tgttcttcgg gacggagtac gtggtccgcc tctggtccgc cggctgccgc agcaagtacg
241tgggcctctg ggggcggctg cgctttgccc ggaagcccat ttccatcatc gacctcatcg
301tggtcgtggc ctccatggtg gtcctctgcg tgggctccaa ggggcaggtg tttgccacgt
361cggccatcag gggcatccgc ttcctgcaga tcctgaggat gctacacgtc gaccgccagg
421gaggcacctg gaggctcctg ggctccgtgg tcttcatcca ccgccaggag ctgataacca
481ccctgtacat cggcttcctg ggcctcatct tctcctcgta ctttgtgtac ctggctgaga
541aggacgcggt gaacgagtca ggccgcgtgg agttcggcag ctacgcagat gcgctgtggt
601ggggggtggt cacagtcacc accatcggct atggggacaa ggtgccccag acgtgggtcg
661ggaagaccat cgcctcctgc ttctctgtct ttgccatctc cttctttgcg ctcccagcgg
721ggattcttgg ctcggggttt gccctgaagg tgcagcagaa gcagaggcag aagcacttca
781accggcagat cccggcggca gcctcactca ttcagaccgc atggaggtgc tatgctgccg
841agaaccccga ctcctccacc tggaagatct acatccggaa ggccccccgg agccacactc
901tgctgtcacc cagccccaaa cccaagaagt ctgtggtggt aaagaaaaaa aagttcaagc
961tggacaaaga caatggggtg actcctggag agaagatgct cacagtcccc catatcacgt
1021gcgacccccc agaagagcgg cggctggacc acttctctgt cgacggctat gacagttctg
1081taaggaagag cccaacactg ctggaagtga gcatgcccca tttcatgaga accaacagct
1141tcgccgagga cctggacctg gaaggggaga ctctgctgac acccatcacc cacatctcac
1201agctgcggga acaccatcgg gccaccatta aggtcattcg acgcatgcag tactttgtgg
1261ccaagaagaa attccagcaa gcgcggaagc cttacgatgt gcgggacgtc attgagcagt
1321actcgcaggg ccacctcaac ctcatggtgc gcatcaagga gctgcagagg aggctggacc
1381agtccattgg gaagccctca ctgttcatcT CCGTCTCAGA AAAGAGCAAG GATCGCGGCA
1441GCAACACGAT CGGCGCCCGC CTGAACCGAG TAGAAGACAA GGTGACGCAG CTGGACCAGA
1501GGCTGGCACT CATCACCGAC ATGCTTCACC AGCTGCTCTC CTTGCACGGT GGCAGCACCC
1561CCGGCAGCGG CGGCCCCCCC AGAGAGGGCG GGGCCCACAT CACCCAGCCC TGCGGCAGTG
1621GCGGCTCCGT CGACCCTGAG CTCTTCCTGC CCAGCAACAC CCTGCCCACC TACGAGCAGC
1681TGACCGTGCC CAGGAGGGGC CCCGATGAGG GGTCCTTGCC AACTTTCTTG TACAAAGTGG
1741TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1801CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1861ACAAGAGGGC TGTCGGTTGT TCTCGACGCG TTAAGTCgac aatcaacctc tggattacaa
1921aatttgtgaa agatt

Download FASTA (ORF) (Full)

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