Construct: ORF TRCN0000479670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008957.1_s317c1
- Derived from:
- ccsbBroadEn_00903
- DNA Barcode:
- CAAGAGGGCTGTCGGTTGTTCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNQ1 (3784)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479670
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3784 | KCNQ1 | potassium voltage-gated cha... | NM_181798.1 | 100% | 100% | |
| 2 | human | 3784 | KCNQ1 | potassium voltage-gated cha... | NM_000218.3 | 81.1% | 80.9% | 1_362del;366_384del;386T>A |
| 3 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_006508492.2 | 82% | 88% | (many diffs) |
| 4 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_017321998.1 | 79.2% | 85% | (many diffs) |
| 5 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | NM_008434.2 | 68.5% | 73.4% | (many diffs) |
| 6 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_006508488.3 | 66.6% | 71.3% | (many diffs) |
| 7 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_011241982.2 | 60.8% | 60.8% | (many diffs) |
| 8 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_006508490.3 | 48.7% | 53% | (many diffs) |
| 9 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_006508491.3 | 48.7% | 53% | (many diffs) |
| 10 | mouse | 16535 | Kcnq1 | potassium voltage-gated cha... | XM_006508489.3 | 47.4% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1716
- ORF length:
- 1647
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacttcctc atcgtcctgg tctgcctcat cttcagcgtg ctgtccacca 121 tcgagcagta tgccgccctg gccacgggga ctctcttctg gatggagatc gtgctggtgg 181 tgttcttcgg gacggagtac gtggtccgcc tctggtccgc cggctgccgc agcaagtacg 241 tgggcctctg ggggcggctg cgctttgccc ggaagcccat ttccatcatc gacctcatcg 301 tggtcgtggc ctccatggtg gtcctctgcg tgggctccaa ggggcaggtg tttgccacgt 361 cggccatcag gggcatccgc ttcctgcaga tcctgaggat gctacacgtc gaccgccagg 421 gaggcacctg gaggctcctg ggctccgtgg tcttcatcca ccgccaggag ctgataacca 481 ccctgtacat cggcttcctg ggcctcatct tctcctcgta ctttgtgtac ctggctgaga 541 aggacgcggt gaacgagtca ggccgcgtgg agttcggcag ctacgcagat gcgctgtggt 601 ggggggtggt cacagtcacc accatcggct atggggacaa ggtgccccag acgtgggtcg 661 ggaagaccat cgcctcctgc ttctctgtct ttgccatctc cttctttgcg ctcccagcgg 721 ggattcttgg ctcggggttt gccctgaagg tgcagcagaa gcagaggcag aagcacttca 781 accggcagat cccggcggca gcctcactca ttcagaccgc atggaggtgc tatgctgccg 841 agaaccccga ctcctccacc tggaagatct acatccggaa ggccccccgg agccacactc 901 tgctgtcacc cagccccaaa cccaagaagt ctgtggtggt aaagaaaaaa aagttcaagc 961 tggacaaaga caatggggtg actcctggag agaagatgct cacagtcccc catatcacgt 1021 gcgacccccc agaagagcgg cggctggacc acttctctgt cgacggctat gacagttctg 1081 taaggaagag cccaacactg ctggaagtga gcatgcccca tttcatgaga accaacagct 1141 tcgccgagga cctggacctg gaaggggaga ctctgctgac acccatcacc cacatctcac 1201 agctgcggga acaccatcgg gccaccatta aggtcattcg acgcatgcag tactttgtgg 1261 ccaagaagaa attccagcaa gcgcggaagc cttacgatgt gcgggacgtc attgagcagt 1321 actcgcaggg ccacctcaac ctcatggtgc gcatcaagga gctgcagagg aggctggacc 1381 agtccattgg gaagccctca ctgttcatcT CCGTCTCAGA AAAGAGCAAG GATCGCGGCA 1441 GCAACACGAT CGGCGCCCGC CTGAACCGAG TAGAAGACAA GGTGACGCAG CTGGACCAGA 1501 GGCTGGCACT CATCACCGAC ATGCTTCACC AGCTGCTCTC CTTGCACGGT GGCAGCACCC 1561 CCGGCAGCGG CGGCCCCCCC AGAGAGGGCG GGGCCCACAT CACCCAGCCC TGCGGCAGTG 1621 GCGGCTCCGT CGACCCTGAG CTCTTCCTGC CCAGCAACAC CCTGCCCACC TACGAGCAGC 1681 TGACCGTGCC CAGGAGGGGC CCCGATGAGG GGTCCTTGCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 ACAAGAGGGC TGTCGGTTGT TCTCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt