Construct: ORF TRCN0000479675
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013280.1_s317c1
- Derived from:
- ccsbBroadEn_03658
- DNA Barcode:
- ATTATTACCGATCCATTACTGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCP1A (55802)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479675
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55802 | DCP1A | decapping mRNA 1A | NM_018403.7 | 100% | 100% | |
| 2 | human | 55802 | DCP1A | decapping mRNA 1A | NM_001290204.2 | 93.4% | 93.4% | 509_510ins114 |
| 3 | human | 55802 | DCP1A | decapping mRNA 1A | NM_001290205.2 | 81.8% | 82.9% | 0_1ins271;34_88del |
| 4 | human | 55802 | DCP1A | decapping mRNA 1A | NM_001290206.2 | 79.2% | 79.2% | 0_1ins363 |
| 5 | human | 55802 | DCP1A | decapping mRNA 1A | NM_001290207.2 | 72.6% | 72.6% | 0_1ins363;146_147ins114 |
| 6 | mouse | 75901 | Dcp1a | decapping mRNA 1A | NM_133761.3 | 85.2% | 86.2% | (many diffs) |
| 7 | mouse | 75901 | Dcp1a | decapping mRNA 1A | XM_006519655.3 | 82.1% | 82.7% | (many diffs) |
| 8 | mouse | 75901 | Dcp1a | decapping mRNA 1A | XM_006519656.2 | 79.4% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1812
- ORF length:
- 1746
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgctgagt cgagctgggc aggagatgag cctagcggcc ctgaagcaac 121 acgaccccta tatcaccagc atcgcagacc tcacgggcca ggtcgctctg tacaccttct 181 gccccaaggc caaccagtgg gagaagactg atatagaagg gaccttattc gtatatcgaa 241 ggtcagcttc cccttaccat ggttttacca ttgtgaatcg actaaatatg cacaatctag 301 ttgaaccagt gaataaagat ttggaatttc agctccatga accatttctt ctgtatagaa 361 atgcaagctt gtcgatatat agtatctggt tttatgacaa gaatgactgt caccgcatag 421 caaaactcat ggctgatgtg gtagaagagg agacacggcg atcccagcaa gctgctcggg 481 acaaacagag tcccagccag gccaatggct gcagcgacca caggcccatc gacatcctgg 541 agatgctgag cagagccaag gatgagtatg agaggaatca gatgggtgac tcaaatatct 601 ccagccctgg gttacagcca agcactcagc tctccaatct gggaagcacc gagactctag 661 aagaaatgcc ctccgggtca caggataagt ctgctccatc tggacacaag catctgacgg 721 tagaagagtt atttggaacc tctttgccaa aggaacaacc agcagttgtg ggtctggatt 781 cagaagaaat ggagaggttg ccaggagatg cctcccagaa agagcccaat tcattcctac 841 catttccctt tgagcagtta ggaggagccc ctcaatcaga aaccctgggt gtcccttctg 901 ctgcccacca ttcagtccag cctgaaatca ccaccccggt gctaatcact ccagcctcca 961 tcacacagtc caatgaaaag catgctccaa cctacacaat cccgttgagc cctgttctca 1021 gtcccactct gccagctgaa gctcctactg cacaggttcc ccccagctta cctcgaaaca 1081 gcaccatgat gcaggcagtg aagaccacgc ctagacagag gtctccactc ctgaaccagc 1141 cagtccctga gctaagccat gccagtctga ttgccaacca gagccccttc agggccccat 1201 tgaacgtgac gaacacagct ggcacatccc tcccaagcgt tgatcttctc cagaaactca 1261 ggttgacccc acagcatgac caaatacaga cacaaccact tgggaaaggt gcaatggtag 1321 ccagcttttc tccggcagct ggtcagctag ccacacctga gagcttcata gagcctccct 1381 ctaagacagc agcagcaaga gtggcggcct cagcctccct gagcaacatg gtgcttgctc 1441 cccttcagtc tatgcagcag aaccaggatc ctgaagtatt tgtgcagccT AAGGTGTTAT 1501 CCAGTGCCAT CCCGGTTGCA GGCGCCCCAC TGGTTACTGC AACGACCACT GCAGTGTCTT 1561 CAGTCCTGCT GGCCCCAAGT GTTTTCCAGC AGACAGTTAC AAGATCTTCG GACCTTGAGA 1621 GGAAAGCCAG CTCCCCTTCT CCTCTAACTA TTGGAACGCC AGAAAGTCAG AGAAAGCCTT 1681 CCATTATTCT CAGCAAGTCT CAGCTCCAGG ATACATTAAT ACATCTAATA AAGAATGATT 1741 CCAGCTTCCT CAGTACACTT CATGAAGTCT ACTTGCAGGT TCTGACCAAG AACAAAGACA 1801 ACCACAACCT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT ATTACCGATC CATTACTGAC 1981 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t