Construct: ORF TRCN0000479683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001030.1_s317c1
- Derived from:
- ccsbBroadEn_02185
- DNA Barcode:
- ACACCGAGTCGGATATACAAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS4B (9525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9525 | VPS4B | vacuolar protein sorting 4 ... | NM_004869.4 | 100% | 100% | |
| 2 | human | 9525 | VPS4B | vacuolar protein sorting 4 ... | XM_011526268.3 | 93.5% | 92.7% | (many diffs) |
| 3 | human | 9525 | VPS4B | vacuolar protein sorting 4 ... | XM_006722582.3 | 73.4% | 72.7% | 0_1ins286;9_10ins68 |
| 4 | human | 9525 | VPS4B | vacuolar protein sorting 4 ... | XR_002958190.1 | 26.9% | (many diffs) | |
| 5 | mouse | 20479 | Vps4b | vacuolar protein sorting 4B | NM_009190.2 | 88.4% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atccacttcg cccaacctcc agaaagcgat agatctggct agcaaagcag 121 cgcaagaaga caaggctggg aactacgaag aagcccttca gctctatcag catgctgtgc 181 agtattttct tcatgtcgtt aaatatgaag cacagggtga taaagccaag caaagtatca 241 gggcaaagtg tacagaatat cttgatagag cagaaaaact aaaggagtac ctgaaaaata 301 aagagaaaaa agcacagaag ccagtgaaag aaggacagcc gagtccagca gatgagaagg 361 ggaatgacag tgatggggaa ggagaatctg atgatcctga aaaaaagaaa ctacagaatc 421 aacttcaagg tgccattgtt atagaacgac caaatgtgaa atggagtgac gttgctggac 481 ttgaaggagc caaagaagca ctgaaagagg ctgtgatact gcctattaaa tttcctcatc 541 tttttacagg caagagaaca ccttggaggg gaatcctatt atttgggccg cctggaacag 601 gaaagtccta cttagccaaa gctgtagcaa cagaagccaa caactcaaca tttttttcaa 661 tatcttcctc tgatcttgtt tctaagtggc taggtgaaag tgaaaaactg gttaagaatt 721 tattccaact tgccagagag aacaagccct ccattatctt cattgatgaa attgattctc 781 tctgtggttc aagaagtgaa aatgaaagtg aagccgcacg tagaattaag acggagttcc 841 tagtgcaaat gcaaggggtt ggtgtagaca atgatggaat tttggttctg ggagctacaa 901 atataccctg ggttctggat tctgccatta ggcgaagatt tgagaaacga atttatattc 961 ccttgccgga accccatgcc cgagcagcaa tgtttaaact gcacctaggg accacTCAGA 1021 ACAGTCTCAC GGAAGCAGAC TTTCGGGAAC TTGGGAGGAA AACAGATGGT TATTCAGGGG 1081 CAGATATAAG TATCATTGTA CGTGATGCCC TTATGCAGCC TGTTAGGAAA GTACAGTCAG 1141 CTACTCATTT TAAAAAGGTT CGCGGACCTT CCCGAGCTGA TCCTAACCAT CTTGTAGATG 1201 ATCTGCTAAC ACCTTGCTCT CCAGGTGACC CTGGTGCCAT TGAAATGACA TGGATGGATG 1261 TCCCTGGAGA TAAACTTTTG GAGCCAGTTG TTTCCATGTC GGATATGTTG CGGTCACTAT 1321 CTAACACAAA ACCTACAGTC AATGAACATG ACTTGTTGAA ATTAAAGAAG TTTACAGAAG 1381 ATTTTGGTCA AGAAGGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACACCGA GTCGGATATA 1561 CAAATCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt