Construct: ORF TRCN0000479692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009220.2_s317c1
- Derived from:
- ccsbBroadEn_07620
- DNA Barcode:
- ATAACACCATCGCCAGTTGTGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAP2 (10486)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10486 | CAP2 | cyclase associated actin cy... | NM_006366.3 | 99.9% | 100% | 1137A>G |
| 2 | human | 10486 | CAP2 | cyclase associated actin cy... | NM_001363534.1 | 94.4% | 94.5% | 222_223ins78;1059A>G |
| 3 | human | 10486 | CAP2 | cyclase associated actin cy... | NM_001363533.1 | 76.4% | 76.5% | 300_301ins336;801A>G |
| 4 | mouse | 67252 | Cap2 | CAP, adenylate cyclase-asso... | NM_026056.4 | 84.3% | 88.8% | (many diffs) |
| 5 | mouse | 67252 | Cap2 | CAP, adenylate cyclase-asso... | XM_006516946.2 | 79.3% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1497
- ORF length:
- 1431
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caacatgcag ggactggtgg aaagactgga acgagctgtc agccgcctgg 121 agtcgctgtc tgcagagtcc cacaggcccc ctgggaactg cggggaagtc aatggtgtca 181 ttgcaggtgt ggcaccctcc gtggaagcct ttgacaagct gatggacagt atggtggccg 241 agtttttaaa gaacagtagg atccttgctg gggacgtgga gacccatgca gaaatggtgc 301 acagtgcttt ccaggcccag cgggctttcc ttctgatggc ctctcagtac caacaacccc 361 acgagaatga cgtggccgca cttctgaaac ccatatcgga aaagattcag gaaatccaaa 421 ctttcagaga gagaaaccgg gggagtaaca tgtttaatca tctttcggcc gtcagcgaaa 481 gcatccctgc ccttggatgg atagctgtgt ctcccaaacc tggtccttat gtcaaggaga 541 tgaatgacgc tgccaccttt tacactaaca gggtcttaaa ggactacaaa cacagtgatt 601 tgcgtcatgt ggattgggtg aagtcatatt tgaacatttg gagtgaactt caagcataca 661 tcaaggaaca ccacaccacg ggcctcacat ggagcaaaac aggtcctgta gcatccacag 721 tatcagcgtt ttctgtcctc tcctctgggc ctggccttcc tccaccccct cctcctctgc 781 ctcctccagg gccacctcca cttttcgaga atgaaggcaa aaaagaggaa tcttctcctt 841 cacgctcagc tttatttgcc caacttaacc agggagaagc aattacaaaa gggctccgcc 901 atgtcacaga tgaccagaag acatacaaaa atcccagcct gcgggctcaa ggagggcaaa 961 ctcaatctcc caccaaaagt cacactccaa gtcccacatc tcctaaatct tatccttctc 1021 aaaaacatgc cccagtgttg gagttggaag gaaagaaatg gagagtggag taccaagagg 1081 acaggaatga ccttgtgatt tcagagactg agctgaaaca agtggcttac attttcaaat 1141 gcgaaaaaTC AACTATTCAG ATAAAAGGGA AAGTAAACTC CATTATAATT GACAACTGTA 1201 AGAAACTCGG CCTGGTGTTT GACAATGTGG TGGGCATTGT GGAAGTGATC AACTCCCAGG 1261 ACATTCAAAT CCAGGTAATG GGGAGAGTGC CAACAATTTC CATTAATAAG ACAGAAGGTT 1321 GCCACATATA CCTCAGTGAA GATGCATTAG ACTGTGAGAT CGTGAGCGCC AAGTCATCTG 1381 AAATGAACAT ACTTATCCCT CAGGATGGTG ATTATAGAGA ATTTCCCATT CCTGAACAGT 1441 TCAAGACAGC ATGGGATGGA TCCAAGTTAA TCACTGAACC TGCAGAAATT ATGGCCTACC 1501 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GAATAACACC ATCGCCAGTT GTGTCTACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt