Construct: ORF TRCN0000479729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015072.2_s317c1
- Derived from:
- ccsbBroadEn_02087
- DNA Barcode:
- TAGCGTTGGACCCATTGGCTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF4 (9128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_004697.4 | 100% | 100% | |
| 2 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001244926.1 | 99.8% | 99.8% | 26_27insGCA |
| 3 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001322266.1 | 52.5% | 52.1% | (many diffs) |
| 4 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001322267.1 | 52.5% | 52.1% | (many diffs) |
| 5 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NR_136265.1 | 51.8% | 1_110del;1206_1207ins49;1628_2875del | |
| 6 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NR_136266.1 | 51.7% | (many diffs) | |
| 7 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | NM_027297.3 | 90.7% | 98.8% | (many diffs) |
| 8 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | XM_006538249.2 | 48.5% | 51.6% | (many diffs) |
| 9 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | XR_390341.1 | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcctcgcga gcctcttcca cgcaggcaac caaaactaaa gcacccgacg 121 acttagttgc tccggtcgtg aagaaaccac acatctatta tggaagtttg gaagagaagg 181 agagggagcg tctggccaaa ggagagtctg ggattttggg gaaagacgga cttaaagcag 241 ggatcgaagc tggaaatatt aatataacct ctggagaagt gtttgaaatt gaagagcata 301 tcagcgagcg acaggcagaa gtattggctg agtttgagag aaggaagcga gcccggcaga 361 tcaatgtttc cacagatgac tcagaggtca aagcttgcct tagagccttg ggggaaccca 421 tcacactttt tggagagggt cctgctgaaa gaagagaaag gttaagaaat atcctctcag 481 ttgtcggtac tgatgccttg aaaaagacca aaaaggatga tgagaagtct aaaaagtcca 541 aagaagagta tcagcaaacc tggtatcatg aaggaccaaa tagcttgaag gtggcaagac 601 tatggattgc taattattcg ttgcccaggg caatgaaacg cttggaagag gcccgactcc 661 ataaggagat tcctgagaca acaaggacct cccagatgca agagctgcac aagtctctcc 721 ggtctttgaa taatttttgc agtcagattg gggatgatcg gcctatctcc tactgtcact 781 ttagtcccaa ttccaagatg ctggccacag cttgttggag tgggctttgc aagctctggt 841 ctgttcctga ttgcaacctc cttcacactc ttcgagggca taacacaaat gtaggagcaa 901 ttgtattcca tcccaaatcc actgtctcct tggacccaaa agatgtcaac ctggcctctt 961 gtgcggctga tggctctgtg aagctttgga gtctcgacag tgatgaacca gtggcagata 1021 ttgaaggcca tacagtgcgt gtggcgcggg taatgtggca tccttcagga cgtttcctgg 1081 gcaccacctg ctatgaccgt tcatggcgct tatgggattt ggaggctcaa gaggagatcc 1141 tgcatcagga aggccatagc atgggtgtgt atgacattgc cttccatcaa gatggctctt 1201 tggctggcac tgggggactg gatgcatttg gtcgagtttg ggacctacgc acaggacgtt 1261 gtatcatgtt cttagaaggc CACCTGAAAG AAATCTATGG AATAAATTTC TCCCCCAATG 1321 GCTATCACAT TGCAACCGGC AGTGGTGACA ACACCTGCAA AGTGTGGGAC CTCCGACAGC 1381 GGCGTTGCGT CTACACCATC CCTGCTCATC AGAACTTAGT GACTGGTGTC AAGTTTGAGC 1441 CTATCCATGG GAACTTCTTG CTTACTGGTG CCTATGATAA CACAGCCAAG ATCTGGACGC 1501 ACCCAGGCTG GTCCCCGCTG AAGACTCTGG CTGGCCACGA AGGCAAAGTG ATGGGCCTAG 1561 ATATTTCTTC CGATGGGCAG CTCATAGCCA CTTGCTCATA TGACAGGACC TTCAAGCTGT 1621 GGATGGCTGA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAG CGTTGGACCC ATTGGCTGGA 1801 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t