Transcript: Human NM_001322266.1

Homo sapiens pre-mRNA processing factor 4 (PRPF4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
PRPF4 (9128)
Length:
2960
CDS:
876..1715

Additional Resources:

NCBI RefSeq record:
NM_001322266.1
NBCI Gene record:
PRPF4 (9128)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322266.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364701 AGACTATGGATTGCTAATTAT pLKO_005 639 5UTR 100% 15.000 21.000 N PRPF4 n/a
2 TRCN0000364756 ATGACCGTTCATGGCGCTTAT pLKO_005 1174 CDS 100% 10.800 15.120 N PRPF4 n/a
3 TRCN0000364755 CCACGAACTGTGTAGACATTG pLKO_005 1945 3UTR 100% 10.800 15.120 N PRPF4 n/a
4 TRCN0000364754 CTACGCACAGGACGTTGTATC pLKO_005 1326 CDS 100% 10.800 15.120 N PRPF4 n/a
5 TRCN0000074769 CGTTGTATCATGTTCTTAGAA pLKO.1 1338 CDS 100% 5.625 7.875 N PRPF4 n/a
6 TRCN0000369441 GGATCGAAGCTGGAAATATTA pLKO_005 283 5UTR 100% 15.000 10.500 N PRPF4 n/a
7 TRCN0000369442 CACAAGTCTCTCCGGTCTTTG pLKO_005 750 5UTR 100% 10.800 7.560 N PRPF4 n/a
8 TRCN0000074772 GCAAAGTGATGGGCCTAGATA pLKO.1 1624 CDS 100% 5.625 3.938 N PRPF4 n/a
9 TRCN0000074771 AGGACGTTGTATCATGTTCTT pLKO.1 1334 CDS 100% 4.950 3.465 N PRPF4 n/a
10 TRCN0000074770 CCTATCTCCTACTGTCACTTT pLKO.1 804 5UTR 100% 4.950 3.465 N PRPF4 n/a
11 TRCN0000074768 GCTCTCTCTAAGGAGCTGTTT pLKO.1 1746 3UTR 100% 4.950 3.465 N PRPF4 n/a
12 TRCN0000364700 ATGAACCAGTGGCAGATATTG pLKO_005 1084 CDS 100% 13.200 7.920 N PRPF4 n/a
13 TRCN0000138391 CGCCTGTAATCCTAGCACTTT pLKO.1 2005 3UTR 100% 4.950 2.475 Y DENND6A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322266.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11339 pDONR223 100% 52.6% 52.2% None (many diffs) n/a
2 ccsbBroad304_11339 pLX_304 0% 52.6% 52.2% V5 (many diffs) n/a
3 TRCN0000478206 TCCATAGACGACGTCGATTACTTT pLX_317 17.1% 52.6% 52.2% V5 (many diffs) n/a
4 ccsbBroadEn_02087 pDONR223 100% 52.5% 52.1% None (many diffs) n/a
5 ccsbBroad304_02087 pLX_304 0% 52.5% 52.1% V5 (many diffs) n/a
6 TRCN0000479729 TAGCGTTGGACCCATTGGCTGGAC pLX_317 22.8% 52.5% 52.1% V5 (many diffs) n/a
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