Transcript: Human NR_136265.1

Homo sapiens pre-mRNA processing factor 4 (PRPF4), transcript variant 5, non-coding RNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
PRPF4 (9128)
Length:
2875
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_136265.1
NBCI Gene record:
PRPF4 (9128)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_136265.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364701 AGACTATGGATTGCTAATTAT pLKO_005 642 3UTR 100% 15.000 21.000 N PRPF4 n/a
2 TRCN0000364756 ATGACCGTTCATGGCGCTTAT pLKO_005 1138 3UTR 100% 10.800 15.120 N PRPF4 n/a
3 TRCN0000364755 CCACGAACTGTGTAGACATTG pLKO_005 1860 3UTR 100% 10.800 15.120 N PRPF4 n/a
4 TRCN0000364754 CTACGCACAGGACGTTGTATC pLKO_005 1241 3UTR 100% 10.800 15.120 N PRPF4 n/a
5 TRCN0000074769 CGTTGTATCATGTTCTTAGAA pLKO.1 1253 3UTR 100% 5.625 7.875 N PRPF4 n/a
6 TRCN0000369441 GGATCGAAGCTGGAAATATTA pLKO_005 286 3UTR 100% 15.000 10.500 N PRPF4 n/a
7 TRCN0000369442 CACAAGTCTCTCCGGTCTTTG pLKO_005 753 3UTR 100% 10.800 7.560 N PRPF4 n/a
8 TRCN0000074772 GCAAAGTGATGGGCCTAGATA pLKO.1 1539 3UTR 100% 5.625 3.938 N PRPF4 n/a
9 TRCN0000074771 AGGACGTTGTATCATGTTCTT pLKO.1 1249 3UTR 100% 4.950 3.465 N PRPF4 n/a
10 TRCN0000074770 CCTATCTCCTACTGTCACTTT pLKO.1 807 3UTR 100% 4.950 3.465 N PRPF4 n/a
11 TRCN0000074768 GCTCTCTCTAAGGAGCTGTTT pLKO.1 1661 3UTR 100% 4.950 3.465 N PRPF4 n/a
12 TRCN0000364700 ATGAACCAGTGGCAGATATTG pLKO_005 1048 3UTR 100% 13.200 7.920 N PRPF4 n/a
13 TRCN0000138391 CGCCTGTAATCCTAGCACTTT pLKO.1 1920 3UTR 100% 4.950 2.475 Y DENND6A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_136265.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02087 pDONR223 100% 51.8% None 1_110del;1206_1207ins49;1628_2875del n/a
2 ccsbBroad304_02087 pLX_304 0% 51.8% V5 1_110del;1206_1207ins49;1628_2875del n/a
3 TRCN0000479729 TAGCGTTGGACCCATTGGCTGGAC pLX_317 22.8% 51.8% V5 1_110del;1206_1207ins49;1628_2875del n/a
4 ccsbBroadEn_11339 pDONR223 100% 51.7% None (many diffs) n/a
5 ccsbBroad304_11339 pLX_304 0% 51.7% V5 (many diffs) n/a
6 TRCN0000478206 TCCATAGACGACGTCGATTACTTT pLX_317 17.1% 51.7% V5 (many diffs) n/a
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