Construct: ORF TRCN0000479754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000419.3_s317c1
- Derived from:
- ccsbBroadEn_15103
- DNA Barcode:
- TATCTCCTATGTTGTTCGCTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAGK (55577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_017567.6 | 99.8% | 99.7% | 273G>C;707G>T |
| 2 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001330425.3 | 84.9% | 84.8% | 0_1ins153;120G>C;554G>T |
| 3 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001330426.2 | 84.9% | 84.8% | 0_1ins153;120G>C;554G>T |
| 4 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001365466.2 | 84.9% | 84.8% | 0_1ins153;120G>C;554G>T |
| 5 | human | 55577 | NAGK | N-acetylglucosamine kinase | XM_011532957.1 | 84.9% | 84.8% | 0_1ins153;120G>C;554G>T |
| 6 | human | 55577 | NAGK | N-acetylglucosamine kinase | XR_939694.1 | 59.8% | (many diffs) | |
| 7 | human | 55577 | NAGK | N-acetylglucosamine kinase | XM_017004440.1 | 55.4% | 49.7% | (many diffs) |
| 8 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_019542.2 | 86.8% | 91.2% | (many diffs) |
| 9 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_001347315.1 | 82.1% | 84.5% | (many diffs) |
| 10 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506408.2 | 77.7% | 81.9% | (many diffs) |
| 11 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_001164187.1 | 73.5% | 77.9% | (many diffs) |
| 12 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506409.2 | 63.4% | 61.8% | (many diffs) |
| 13 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506410.1 | 63.4% | 61.8% | (many diffs) |
| 14 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_017321685.1 | 63.4% | 61.8% | (many diffs) |
| 15 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_017321686.1 | 38.6% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1098
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgatctat gggggtgtag aggggggagg cacacgatcc gaggtccttt 121 tagtctcaga ggatgggaag atcctggcag aagcagatgg actgagcaca aaccactggc 181 tgatcgggac agacaagtgt gtggagagga tcaatgagat ggtgaacagg gccaaacgga 241 aagcaggggt ggatcctctg gtaccgctgc gaagcttggg cctatctctg agcggtgggg 301 accaggagga cgcggggagg atcctgatcg aggagctcag ggaccgattt ccctacctga 361 gtgaaagcta cttaatcacc accgatgccg ccggctccat cgccacagct acaccggatg 421 gtggagttgt gctcatatct ggaacaggct ccaactgcag gctcatcaac cctgatggct 481 ccgagagtgg ctgcggcggc tggggccata tgatgggtga tgagggttca gcctactgga 541 tcgcacacca agcagtgaaa atagtgtttg actccattga caacctagag gcggctcctc 601 atgatatcgg ctacgtcaaa caggccatgt tccactattt ccaggtgcca gatcggctag 661 ggatactcac tcacctgtat agggactttg ataaatgcag gtttgctggg ttttgccgga 721 aaattgcaga aggtgcTCAG CAGGGAGACC CCCTTTCCCG CTATATCTTC ATGAAGGCTG 781 GGGAGATGCT GGGCAGACAC ATCGTAGCAG TGTTGCCCGA GATTGACCCG GTCTTGTTCC 841 AGGGCAAGAT TGGACTCCCC ATCCTGTGCG TGGGCTCTGT GTGGAAGAGC TGGGAGCTGC 901 TGAAGGAAGG TTTTCTTCTG GCGCTGACCC AGGGCAGAGA GATCCAGGCT CAGAACTTCT 961 TCTCCAGCTT CACCCTGATG AAGCTGAGGC ACTCCTCCGC TCTGGGTGGG GCCAGCCTAG 1021 GGGCCAGGCA CATCGGGCAC CTCCTCCCCA TGGACTATAG CGCCAATGCC ATTGCCTTCT 1081 ATTCCTACAC CTTTTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATATCTCC TATGTTGTTC 1261 GCTGGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt