Construct: ORF TRCN0000479773
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012092.1_s317c1
- Derived from:
- ccsbBroadEn_14104
- DNA Barcode:
- TTGCCTTGAAAGTTTAACACCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSBPL3 (26031)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479773
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NM_015550.4 | 99.9% | 100% | 753G>A |
| 2 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_005249698.3 | 99.9% | 100% | 753G>A |
| 3 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_011515258.2 | 99.9% | 100% | 753G>A |
| 4 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NM_145320.2 | 96.4% | 96.5% | 753G>A;774_775ins93 |
| 5 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_006715681.3 | 96.4% | 96.5% | 753G>A;774_775ins93 |
| 6 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NM_145321.2 | 95.9% | 95.9% | 753G>A;1158_1159ins108 |
| 7 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_006715682.3 | 95.9% | 95.9% | 753G>A;1158_1159ins108 |
| 8 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_011515259.2 | 95% | 95% | 0_1ins132;621G>A |
| 9 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_017011948.1 | 95% | 95% | 0_1ins132;621G>A |
| 10 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NM_145322.2 | 92.4% | 92.4% | 753G>A;774_775ins93;1065_1066ins108 |
| 11 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_006715683.3 | 92.4% | 92.4% | 753G>A;774_775ins93;1065_1066ins108 |
| 12 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_017011949.1 | 90.9% | 90.9% | 0_1ins132;621G>A;1026_1027ins108 |
| 13 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_926927.2 | 65.9% | (many diffs) | |
| 14 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XM_006715684.4 | 60.2% | 60.2% | 0_1ins1059 |
| 15 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_926929.1 | 48.3% | (many diffs) | |
| 16 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NR_104111.1 | 38.7% | (many diffs) | |
| 17 | human | 26031 | OSBPL3 | oxysterol binding protein l... | NR_104112.1 | 37.4% | (many diffs) | |
| 18 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_428073.3 | 37.3% | (many diffs) | |
| 19 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_428074.3 | 37% | (many diffs) | |
| 20 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_428075.3 | 35.7% | (many diffs) | |
| 21 | human | 26031 | OSBPL3 | oxysterol binding protein l... | XR_001744620.1 | 35.5% | (many diffs) | |
| 22 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | NM_001347213.1 | 86.1% | 91.4% | (many diffs) |
| 23 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506630.3 | 86.1% | 91.4% | (many diffs) |
| 24 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506631.3 | 86.1% | 91.4% | (many diffs) |
| 25 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_011241468.2 | 86.1% | 91.4% | (many diffs) |
| 26 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | NM_027881.3 | 82.8% | 88.1% | (many diffs) |
| 27 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506633.3 | 82.8% | 88.1% | (many diffs) |
| 28 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506634.3 | 82.4% | 87.7% | (many diffs) |
| 29 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | NM_001163645.1 | 79% | 84.4% | (many diffs) |
| 30 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506635.3 | 70.1% | 75.4% | (many diffs) |
| 31 | mouse | 71720 | Osbpl3 | oxysterol binding protein-l... | XM_006506636.3 | 50.4% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2727
- ORF length:
- 2661
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gagtgatgag aagaaccttg gtgtgtccca aaaattggta tcaccttcaa 121 ggagcacaag tagctgctct tccaagcaag gaagtcgaca ggacagctgg gaagtggtgg 181 aaggactgag gggggagatg aattacaccc aggagccacc agttcagaaa ggatttttgc 241 tgaaaaagag gaagtggccc ttaaaaggct ggcataagag attcttctat ctggacaaag 301 gaatcttgaa atatgccaag agccaaaccg atatagagag agagaagctg catggctgca 361 ttgatgtcgg gctctcagtg atgtctgtaa agaagtcatc aaaatgcata gaccttgaca 421 ccgaggagca catctaccat ctgaaggtca agtcagaaga agtctttgat gagtgggtat 481 cgaaacttcg ccaccacaga atgtatcgtc agaatgaaat tgccatgttt ccacatgaag 541 ttaaccactt tttctcaggg tccaccatca cagactcttc atctggggtg tttgactcca 601 tttcaagtag gaagcgtagc agtatatcaa agcagaattt atttcaaact ggaagcaatg 661 tatcattttc ttgtggtggt gagacacgag ttccattatg gttacagtct tcagaggaca 721 tggaaaaatg ctccaaagac ctggcgcact gtcatgccta cctggtagaa atgagccagc 781 tcctgcaaag catggacgtc ctgcatcgga catactcagc accagctatc aacgccatcc 841 agggtggatc ttttgaaagt cccaaaaagg aaaaaagatc gcacaggagg tggcggtcca 901 gagctattgg caaagatgct aaaggaacac tgcaggtccc gaaacctttt tctggcccag 961 taagactaca ctcctccaat cctaatttgt caacactaga ttttggagaa gagaaaaatt 1021 attctgatgg ctctgaaacc tcatcagagt tttctaaaat gcaagaagat ctgtgtcata 1081 ttgcccataa agtttacttc actttaaggt cagcttttaa tatcatgtca gcggagagag 1141 agaaactgaa gcagctgatg gagcaggatg cctcctcctc cccgtctgct caggtcattg 1201 gtctgaagaa cgccctgtca tccgccctag cacaaaacac agatcttaaa gaacgcttac 1261 gcagaatcca tgccgagtct ctgctcctcg actcccccgc tgtcgccaag tcgggtgaca 1321 atctggcaga ggaaaactcc agagatgaaa accgagctct agttcatcag ctttctaatg 1381 aaagtagact ctccatcact gactcccttt ctgagttttt tgatgctcag gaagttctgt 1441 tatctccaag ctcttcagaa aacgagattt ctgatgatga ctcatatgtc agtgacataa 1501 gtgataatct ttccttagat aatctcagta atgatttaga taatgagaga cagaccttgg 1561 ggcctgtcct tgatagtggt cgggaagcga agtcccggag aagaacgtgc ctgccggcgc 1621 cctgcccgag cagcagtaac atcagcctgt ggaacatcct gaggaacaac atcgggaagg 1681 acctgtccaa ggtggccatg ccggtggagc tgaacgagcc cctgaacacg ctgcagaggc 1741 tctgcgagga gctggagtac agcgagctcc tggacaaggc cgcgcagatt cccagccccc 1801 tggaaaggat ggtatatgtg gcagcctttg ccatatcagc gtatgcatct agctactacc 1861 gagctggaag caagccattt aatccggttc ttggagaaac atatgaatgt attcgggagg 1921 acaagggctt ccagtttttt tcagaacagg tcagccacca tccgcctatc tctgcgtgtc 1981 atgctgagtc tagaaatttt gttttctggc aagatgtgag atggaaaaac aaattctggg 2041 gcaaatccat ggaaattgtt ccaattggca caacccatgt gactctgcca gtttttgggg 2101 atcattttga gtggaacaaa gtgacctctt gcatccataa catcttaagc gggcagaggt 2161 ggattgagca ctatggagag attgtcatca agaacctgca tgatgattcc tgctactgca 2221 aagtgaattt tataaaggca aaatactgga gcactaatgc ccatgagatt gaaggcacag 2281 tgtttgacag gagtggaaaa gcggttcatc ggctgtttgg gaaatggcat gaaagcatct 2341 actgtggcgg cggctcctct tctgcctgtg tatggagagc aaaTCCTATG CCGAAAGGCT 2401 ACGAGCAATA CTATAGCTTC ACACAGTTTG CGCTGGAATT AAATGAAATG GATCCATCAT 2461 CAAAGTCTTT ATTGCCACCT ACTGACACTC GATTTAGGCC AGACCAGAGG TTTCTAGAGG 2521 AAGGGAACTT AGAAGAAGCT GAAATACAAA AGCAGAGGAT TGAACAACTG CAGAGAGAAA 2581 GGCGGCGGGT CTTAGAAGAA AATCATGTGG AGCACCAGCC TCGGTTTTTC AGGAAATCCG 2641 ACGATGACTC TTGGGTGAGC AACGGCACCT ATTTGGAACT TAGAAAAGAT CTTGGTTTTT 2701 CCAAACTGGA CCATCCTGTC TTATGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 2761 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2821 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTGCCTTG 2881 AAAGTTTAAC ACCAATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2941 aagatt