Transcript: Mouse XM_006506633.3

PREDICTED: Mus musculus oxysterol binding protein-like 3 (Osbpl3), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Osbpl3 (71720)
Length:
7071
CDS:
766..3333

Additional Resources:

NCBI RefSeq record:
XM_006506633.3
NBCI Gene record:
Osbpl3 (71720)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506633.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105315 CCTGTAACTTTGCCCACATTT pLKO.1 3356 3UTR 100% 13.200 18.480 N Osbpl3 n/a
2 TRCN0000105317 CCCGGAAGCAATTTGTCATTT pLKO.1 1345 CDS 100% 13.200 9.240 N Osbpl3 n/a
3 TRCN0000105319 CTCGGTTTAGACCAGATCAAA pLKO.1 3092 CDS 100% 5.625 3.938 N Osbpl3 n/a
4 TRCN0000105318 CCTCAGTAATGATTTAGACAA pLKO.1 2127 CDS 100% 4.950 3.465 N Osbpl3 n/a
5 TRCN0000105316 CCACAAAGTTTACTTCGCTTT pLKO.1 1689 CDS 100% 4.050 2.835 N Osbpl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506633.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000479773 TTGCCTTGAAAGTTTAACACCAAT pLX_317 13.2% 82.8% 88.1% V5 (many diffs) n/a
2 ccsbBroadEn_14104 pDONR223 100% 82.7% 87.9% None (many diffs) n/a
3 ccsbBroad304_14104 pLX_304 0% 82.7% 87.9% V5 (not translated due to frame shift) (many diffs) n/a
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