Construct: ORF TRCN0000479792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001825.1_s317c1
- Derived from:
- ccsbBroadEn_04751
- DNA Barcode:
- CCTGGGCTACGAACCGCCCCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEDD1 (121441)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | NM_001135176.2 | 100% | 100% | |
| 2 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | NM_152905.4 | 100% | 100% | |
| 3 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | NM_001135175.1 | 98.9% | 98.9% | 1_21del |
| 4 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_005268644.2 | 98.9% | 98.9% | 1_21del |
| 5 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | NM_001135177.2 | 86.5% | 86.5% | 0_1ins267 |
| 6 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_006719237.4 | 86.5% | 86.5% | 0_1ins267 |
| 7 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_011537903.3 | 86.5% | 86.5% | 0_1ins267 |
| 8 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_011537904.3 | 77.5% | 76.7% | (many diffs) |
| 9 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_011537905.3 | 77.5% | 76.7% | (many diffs) |
| 10 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_017018801.2 | 77.5% | 76.7% | (many diffs) |
| 11 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_011537906.3 | 75.7% | 75.3% | (many diffs) |
| 12 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_017018802.2 | 75.7% | 75.3% | (many diffs) |
| 13 | human | 121441 | NEDD1 | NEDD1 gamma-tubulin ring co... | XM_024448835.1 | 75.7% | 75.3% | (many diffs) |
| 14 | mouse | 17997 | Nedd1 | neural precursor cell expre... | NM_008682.2 | 85.2% | 85.4% | (many diffs) |
| 15 | mouse | 17997 | Nedd1 | neural precursor cell expre... | XM_006513334.1 | 80.4% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2046
- ORF length:
- 1980
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggaaaacctc agatttgctt catcaggaga tgatattaaa atatgggatg 121 cttcatctat gacattggtg gataaattca acccacacac atcaccacat ggaatcagct 181 caatatgttg gagcagcaat aataactttt tagtaacagc atcttccagt ggcgacaaaa 241 tagttgtctc aagttgcaaa tgtaaacctg ttccactttt agagcttgct gaagggcaaa 301 agcagacatg tgtcaattta aattctacat ctatgtattt ggtaagcgga ggcctaaata 361 acactgttaa tatttgggat ttaaaatcaa aaagagttca tcgatctctt aaggatcata 421 aagatcaagt aacttgtgta acatacaatt ggaatgattg ctacattgct tctggatctc 481 ttagtggtga aattatttta cacagtgtaa ccactaattt atctagtact ccttttggcc 541 atggtagtaa ccagtctgtt cggcacttga agtactcctt gtttaagaaa tcactactgg 601 gcagtgtttc ggataatgga atagtaactc tctgggatgt aaatagtcag agtccatacc 661 ataactttga cagtgtacac aaagctccag cgtcaggcat ctgtttttct cctgtcaatg 721 aattgctctt tgtaaccata ggcttggata aaagaatcat cctctatgac acttcaagta 781 agaagctagt gaaaacttta gtggctgaca ctcctctaac tgcggtagat ttcatgcctg 841 atggagccac tttggctatt ggatcttccc gggggaaaat atatcaatat gatttaagaa 901 tgttgaaatc accagttaag accatcagtg ctcacaagac atctgtgcag tgtatagcat 961 ttcagtactc cactgttctt actaagtcaa gtttaaataa aggctgttca aataagccca 1021 caacagtgaa caaacgaagt gttaatgtga atgctgctag tggaggagtt cagaattccg 1081 gaattgtcag agaagcacct gccacgtcca ttgccacagt tctaccacaa cctatgacat 1141 cagctatggg gaaaggaaca gttgctgttc aagaaaaagc aggtttgcct cgaagcataa 1201 acacagacac tttatctaag gaaacagaca gtggaaaaaa tcaggatttc tccagctttg 1261 atgatactgg gaaaagtagt ttaggtgaca tgttctcacc tatcagagat gatgctgtag 1321 ttaacaaggg aagtgatgag tccataggca aaggagatgg ctttgacttt ctaccgcagt 1381 tgaactcagt gtttcctcca agaaaaaatc cagtaacttc aagtacttca gtattgcatt 1441 ctagtcctct taatgttttt atgggatctc cagggaaaga ggaaaatgaa aaccgtgatc 1501 taacagctga gtctaagaaa atatatatgg gaaaacagga atctaaagac tccttcaaac 1561 agttagcaaa gttggtcaca tctggtgctg aaagtggaaa tctaaatacc tctccatcat 1621 ctaaccaaac aagaaattcT GAGAAATTTG AAAAGCCAGA GAATGAAATT GAAGCCCAGT 1681 TGATATGTGA ACCCCCAATC AATGGATCCT CAACTCCAAA TCCAAAGATA GCATCTTCTG 1741 TCACTGCTGG AGTTGCCAGT TCACTCTCAG AAAAAATAGC CGACAGCATT GGAAATAACC 1801 GGCAAAATGC ACCATTGACT TCCATTCAAA TTCGTTTTAT TCAGAACATG ATACAGGAAA 1861 CGTTGGATGA CTTTAGAGAA GCATGCCATA GGGACATTGT GAATTTGCAA GTGGAGATGA 1921 TTAAACAGTT TCATATGCAA CTGAATGAAA TGCATTCTTT GCTGGAAAGA TACTCAGTGA 1981 ATGAAGGTTT AGTGGCTGAA ATTGAAAGAC TACGAGAAGA AAACAAAAGA TTACGGGCCC 2041 ACTTTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTGGGCTA CGAACCGCCC CACCTACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt