Construct: ORF TRCN0000479837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002645.1_s317c1
- Derived from:
- ccsbBroadEn_08553
- DNA Barcode:
- ATTGAATCGACACTATGGGCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAP2 (55620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55620 | STAP2 | signal transducing adaptor ... | NM_001013841.2 | 99.9% | 99.7% | 277G>A |
| 2 | human | 55620 | STAP2 | signal transducing adaptor ... | XM_011528123.1 | 89.8% | 89.7% | 277G>A;1069_1203del |
| 3 | human | 55620 | STAP2 | signal transducing adaptor ... | NM_017720.3 | 89.6% | 89.5% | 277G>A;1069_1206del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctgccctg aggccacccc gtgtccccaa gcctaagggt gtcctgcctt 121 cacactacta tgagagcttt ctagagaaga aggggccctg tgaccgggat tacaagaagt 181 tctgggcagg cctgcagggt ctcaccattt atttctacaa tagcaatcgg gacttccagc 241 acgtggagaa gctcaacttg ggagcatttg agaaactcac agatgagatt ccctggggaa 301 gctcacgtga ccctggcacc cacttcagcc tgattctccg gaatcaggag atcaagttca 361 aggtagagac cttggagtgt cgggaaatgt ggaaaggctt catcttaacg gtggtggagc 421 tccgtgtccc gaccgacttg accctgcttc ctgggcacct atacatgatg tctgaagtct 481 tggccaaaga ggaggcgcgc cgtgcactgg agacaccctc gtgcttcctg aaggtgagcc 541 ggctggaggc acaactgctc ctggagcgct accccgagtg cgggaacctg ctgctgcggc 601 ccagcgggga cggcgccgac ggcgtgtcgg tcaccacgcg gcagatgcac aacgggacgc 661 acgtggtccg gcattacaag gtgaagcggg agggccccaa gtacgtgatc gatgtggaac 721 agccgttctc ttgcacctcc ctggacgccg tggtcaacta tttcgtgtcg cataccaaaa 781 aggcgctggt gccattcctg ttagacgagg actacgagaa ggtgctaggc tacgtggaag 841 ccgataagga gaatggcgag aatgtgtggg tggcgccctc cgctccgggc ccaggtcctg 901 caccctgcac aggtggcccc aagccgctgt caccTGCGTC TAGCCAGGAC AAGCTGCCCC 961 CACTGCCCCC ACTACCGAAC CAGGAAGAGA ACTACGTGAC CCCCATTGGA GATGGCCCAG 1021 CTGTTGACTA TGAGAACCAA GATGTGGCTT CCTCTAGTTG GCCAGTCATC CTGAAGCCAA 1081 AGAAGTTGCC AAAGCCTCCT GCCAAGCTTC CAAAGCCACC CGTTGGACCC AAGCCAGAGC 1141 CCAAAGTCTT TAATGGTGGC TTGGGCAGGA AGCTGCCAGT CAGTTCAGCC CAGCCTCTCT 1201 TCCCCACAGC CGGGCTGGCA GACATGACGG CAGAGCTACA GAAGAAGCTG GAGAAGAGGC 1261 GGGCACTGGA GCACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTGAATCGA CACTATGGGC 1441 ACAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt