Construct: ORF TRCN0000479854
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002052.1_s317c1
- Derived from:
- ccsbBroadEn_13891
- DNA Barcode:
- GATATTGTCGAACACGCCTCACTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LCK (3932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479854
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001042771.2 | 94.3% | 93.6% | 9T>C;963_964ins90;1516C>T |
| 2 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_005356.5 | 94.3% | 93.6% | 9T>C;963_964ins90;1516C>T |
| 3 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447046.1 | 85.1% | 84.5% | (many diffs) |
| 4 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447047.1 | 85.1% | 84.5% | (many diffs) |
| 5 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001330468.2 | 84.8% | 84% | (many diffs) |
| 6 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_011541453.3 | 76.6% | 75.8% | (many diffs) |
| 7 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162433.1 | 83.5% | 90.7% | (many diffs) |
| 8 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_010693.3 | 83.5% | 90.7% | (many diffs) |
| 9 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_006502818.3 | 83.5% | 90.7% | (many diffs) |
| 10 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253275.1 | 83.5% | 90.7% | (many diffs) |
| 11 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253276.1 | 83.5% | 90.7% | (many diffs) |
| 12 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162432.1 | 81.9% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1671
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcggctgc agctcacacc cggaagatga ctggatggaa aacatcgatg 121 tgtgtgagaa ctgccattat cccatagtcc cactggatgg caagggcacg ctgctcatcc 181 gaaatggctc tgaggtgcgg gacccactgg ttacctacga aggctccaat ccgccggctt 241 ccccactgca agacaacctg gttatcgctc tgcacagcta tgagccctct cacgacggag 301 atctgggctt tgagaagggg gaacagctcc gcatcctgga gcagagcggc gagtggtgga 361 aggcgcagtc cctgaccacg ggccaggaag gcttcatccc cttcaatttt gtggccaaag 421 cgaacagcct ggagcccgaa ccctggttct tcaagaacct gagccgcaag gacgcggagc 481 ggcagctcct ggcgcccggg aacactcacg gctccttcct catccgggag agcgagagca 541 ccgcgggatc gttttcactg tcggtccggg acttcgacca gaaccaggga gaggtggtga 601 aacattacaa gatccgtaat ctggacaacg gtggcttcta catctcccct cgaatcactt 661 ttcccggcct gcatgaactg gtccgccatt acaccaatgc ttcagatggg ctgtgcacac 721 ggttgagccg cccctgccag acccagaagc cccagaagcc gtggtgggag gacgagtggg 781 aggttcccag ggagacgctg aagctggtgg agcggctggg ggctggacag ttcggggagg 841 tgtggatggg gtactacaac gggcacacga aggtggcggt gaagagcctg aagcagggca 901 gcatgtcccc ggacgccttc ctggccgagg ccaacctcat gaagcagctg caacaccagc 961 ggctggttcg gctctacgct gtggtcaccc aggagcccat ctacatcatc actgaataca 1021 tggagaatga cacacttcta gactcccagt tggaggagaa aggtctgggg gcctccccct 1081 ggggcaactt gggccagcaa ctcttgcttc tgcccacagg gagtctagtg gattttctca 1141 agaccccttc aggcatcaag ttgaccatca acaaactcct ggacatggca gcccaaattg 1201 cagaaggcat ggcattcatt gaagagcgga attatattca tcgtgacctt cgggctgcca 1261 acattctggt gtctgacacc ctgagctgca agattgcaga ctttggccta gcacgccTCA 1321 TTGAGGACAA CGAGTACACA GCCAGGGAGG GGGCCAAGTT TCCCATTAAG TGGACAGCGC 1381 CAGAAGCCAT TAACTACGGG ACATTCACCA TCAAGTCAGA TGTGTGGTCT TTTGGGATCC 1441 TGCTGACGGA AATTGTCACC CACGGCCGCA TCCCTTACCC AGGGATGACC AACCCGGAGG 1501 TGATTCAGAA CCTGGAGCGA GGCTACCGCA TGGTGCGCCC TGACAACTGT CCAGAGGAGC 1561 TGTACCAACT CATGAGGCTG TGCTGGAAGG AGCGCCCAGA GGACCGGCCC ACCTTTGACT 1621 ACCTGCGCAG TGTGCTGGAG GACTTCTTCA CGGCCACAGA GGGCCAGTAC TAGCCTCAGC 1681 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1741 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1801 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TATTGTCGAA CACGCCTCAC TGACGCGTTA 1861 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt