Construct: ORF TRCN0000479857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000494.1_s317c1
- Derived from:
- ccsbBroadEn_06166
- DNA Barcode:
- ACCATACGAGCTATAAAGGCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STOM (2040)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479857
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2040 | STOM | stomatin | NM_004099.6 | 99.8% | 99.6% | 15G>N |
| 2 | human | 2040 | STOM | stomatin | NM_001270526.2 | 68.2% | 67.7% | 15G>N;588_589insGTG;591_592ins270 |
| 3 | human | 2040 | STOM | stomatin | NM_001270527.1 | 57% | 56.9% | (many diffs) |
| 4 | human | 2040 | STOM | stomatin | NM_198194.3 | 42.5% | 42.3% | 15G>N;162_163ins495 |
| 5 | human | 2040 | STOM | stomatin | NR_073037.2 | 26.3% | (many diffs) | |
| 6 | mouse | 13830 | Stom | stomatin | NM_013515.2 | 81.4% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagaagcgn cacacacggg actccgaagc ccagcggctc cccgactcct 121 tcaaggacag ccccagtaag ggccttggac cttgcggatg gattttggtg gcgttctcat 181 tcttattcac cgttataact ttcccaatct caatatggat gtgcataaag attataaaag 241 agtatgaaag agccatcatc tttagattgg gtcgcatttt acaaggagga gccaaaggac 301 ctggtttgtt ttttattctg ccatgcactg acagcttcat caaagtggac atgagaacta 361 tttcatttga tattcctcct caggagatcc tcacaaagga ttcagtgaca attagcgtgg 421 atggtgtggt ctattaccgc gttcagaatg caaccctggc tgtggcaaat atcaccaacg 481 ctgactcagc aacccgtctt ttggcacaaa ctactctgag gaatgttctg ggcaccaaga 541 atctttctca gaTCCTCTCT GACAGAGAAG AAATTGCACA CAACATGCAG TCTACTCTGG 601 ATGATGCCAC TGATGCCTGG GGAATAAAGG TGGAGCGTGT GGAAATTAAG GATGTGAAAC 661 TACCTGTGCA GCTCCAGAGA GCTATGGCTG CAGAAGCAGA AGCGTCCCGC GAGGCCCGCG 721 CCAAGGTTAT TGCAGCCGAA GGAGAAATGA ATGCATCCAG GGCTCTGAAA GAAGCCTCCA 781 TGGTCATCAC TGAATCTCCT GCAGCCCTTC AGCTCCGATA CCTGCAGACA CTGACCACCA 841 TTGCTGCTGA GAAAAACTCA ACAATTGTCT TCCCTCTGCC CATAGATATG CTGCAAGGAA 901 TCATAGGGGC AAAACACAGC CATCTAGGCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCAT 1081 ACGAGCTATA AAGGCACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt