Construct: ORF TRCN0000479857

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000494.1_s317c1
Derived from:
ccsbBroadEn_06166
DNA Barcode:
ACCATACGAGCTATAAAGGCACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STOM (2040)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479857
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2040 STOM stomatin NM_004099.6 99.8% 99.6% 15G>N
2 human 2040 STOM stomatin NM_001270526.2 68.2% 67.7% 15G>N;588_589insGTG;591_592ins270
3 human 2040 STOM stomatin NM_001270527.1 57% 56.9% (many diffs)
4 human 2040 STOM stomatin NM_198194.3 42.5% 42.3% 15G>N;162_163ins495
5 human 2040 STOM stomatin NR_073037.2 26.3% (many diffs)
6 mouse 13830 Stom stomatin NM_013515.2 81.4% 85.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
930
ORF length:
864
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgagaagcgn cacacacggg actccgaagc ccagcggctc cccgactcct
121tcaaggacag ccccagtaag ggccttggac cttgcggatg gattttggtg gcgttctcat
181tcttattcac cgttataact ttcccaatct caatatggat gtgcataaag attataaaag
241agtatgaaag agccatcatc tttagattgg gtcgcatttt acaaggagga gccaaaggac
301ctggtttgtt ttttattctg ccatgcactg acagcttcat caaagtggac atgagaacta
361tttcatttga tattcctcct caggagatcc tcacaaagga ttcagtgaca attagcgtgg
421atggtgtggt ctattaccgc gttcagaatg caaccctggc tgtggcaaat atcaccaacg
481ctgactcagc aacccgtctt ttggcacaaa ctactctgag gaatgttctg ggcaccaaga
541atctttctca gaTCCTCTCT GACAGAGAAG AAATTGCACA CAACATGCAG TCTACTCTGG
601ATGATGCCAC TGATGCCTGG GGAATAAAGG TGGAGCGTGT GGAAATTAAG GATGTGAAAC
661TACCTGTGCA GCTCCAGAGA GCTATGGCTG CAGAAGCAGA AGCGTCCCGC GAGGCCCGCG
721CCAAGGTTAT TGCAGCCGAA GGAGAAATGA ATGCATCCAG GGCTCTGAAA GAAGCCTCCA
781TGGTCATCAC TGAATCTCCT GCAGCCCTTC AGCTCCGATA CCTGCAGACA CTGACCACCA
841TTGCTGCTGA GAAAAACTCA ACAATTGTCT TCCCTCTGCC CATAGATATG CTGCAAGGAA
901TCATAGGGGC AAAACACAGC CATCTAGGCT ACCCAACTTT CTTGTACAAA GTGGTTGATA
961TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1021CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCAT
1081ACGAGCTATA AAGGCACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1141tgaaagatt

Download FASTA (ORF) (Full)