Construct: ORF TRCN0000479912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014792.1_s317c1
- Derived from:
- ccsbBroadEn_10683
- DNA Barcode:
- GGTACCCAGATTCAGTATATAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APP (351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479912
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 351 | APP | amyloid beta precursor protein | XM_024452075.1 | 54.4% | 53.3% | (many diffs) |
| 2 | human | 351 | APP | amyloid beta precursor protein | NM_001204303.2 | 44.4% | 42.9% | (many diffs) |
| 3 | human | 351 | APP | amyloid beta precursor protein | NM_201414.3 | 43.3% | 41.8% | (many diffs) |
| 4 | human | 351 | APP | amyloid beta precursor protein | NM_001204302.2 | 40.5% | 39.6% | (many diffs) |
| 5 | human | 351 | APP | amyloid beta precursor protein | NM_201413.3 | 39.5% | 38.7% | (many diffs) |
| 6 | human | 351 | APP | amyloid beta precursor protein | NM_001204301.2 | 39.5% | 38.6% | (many diffs) |
| 7 | human | 351 | APP | amyloid beta precursor protein | NM_000484.4 | 38.5% | 37.7% | (many diffs) |
| 8 | human | 351 | APP | amyloid beta precursor protein | NM_001136131.2 | 38.2% | 36.8% | (many diffs) |
| 9 | human | 351 | APP | amyloid beta precursor protein | NM_001136016.3 | 37.3% | 36.5% | (many diffs) |
| 10 | human | 351 | APP | amyloid beta precursor protein | NM_001136129.3 | 35.2% | 33.8% | (many diffs) |
| 11 | human | 351 | APP | amyloid beta precursor protein | NM_001136130.3 | 31.3% | 30.5% | (many diffs) |
| 12 | mouse | 11820 | App | amyloid beta (A4) precursor... | XM_006522874.1 | 45.9% | 48.4% | (many diffs) |
| 13 | mouse | 11820 | App | amyloid beta (A4) precursor... | XM_006522873.2 | 39.1% | 41.2% | (many diffs) |
| 14 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_007471.3 | 38.1% | 40.1% | (many diffs) |
| 15 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198825.1 | 36.1% | 38% | (many diffs) |
| 16 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198824.1 | 35.2% | 37.1% | (many diffs) |
| 17 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198826.1 | 35.2% | 37.1% | (many diffs) |
| 18 | mouse | 11820 | App | amyloid beta (A4) precursor... | NM_001198823.1 | 34.3% | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcccggtttg gcactgctcc tgctggccgc ctggacggct cgggcgctgg 121 aggtacccac tgatggtaat gctggcctgc tggctgaacc ccagattgcc atgttctgtg 181 gcagactgaa catgcacatg aatgtccaga atgggaagtg ggattcagat ccatcaggga 241 ccaaaacctg cattgatacc aaggaaggca tcctgcagta ttgccaagaa gtctaccctg 301 aactgcagat caccaatgtg gtagaagcca accaaccagt gaccatccag aactggtgca 361 agcggggccg caagcagtgc aagacccatc cccactttgt gattccctac cgctgcttag 421 ttggtgagtt tgtaagtgat gcccttctcg ttcctgacaa gtgcaaattc ttacaccagg 481 agaggatgga tgtttgcgaa actcatcttc actggcacac cgtcgccaaa gagacatgca 541 gtgagaagag taccaacttg catgactacg gcatgttgct gcccTGCGGA ATTGACAAGT 601 TCCGAGGGGT AGAGTTTGTG TGTTGCCCAC TGGCTGAAGA AAGTGACAAT GTGGATTCTG 661 CTGATGCGGA GGAGGATGAC TCGGATGTCT GGTGGGGCGG AGCAGACACA GACTATGCAG 721 ATGGGAGTGA AGACAAAGTA GTAGAAGTAG CAGAGGAGGA AGAAGTGGCT GAGGTGGAAG 781 AAGAAGAAGC CGATGATGAC GAGGACGATG AGGATGGTGA TGAGGTAGAG GAAGAGGCTG 841 AGGAACCCTA CGAAGAAGCC ACAGAGAGAA CCACCAGCAT TGCCACCACC ACCACCACCA 901 CCACAGAGTC TGTGGAAGAG GTGGTTCGAG AGAAGTGGTA TAAGGAAGTA CATTCTGGCC 961 AGGCACGATG GCTCATGCTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGTA CCCAGATTCA 1141 GTATATAAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt