Transcript: Human NM_001136129.3

Homo sapiens amyloid beta precursor protein (APP), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-07-16
Taxon:
Homo sapiens (human)
Gene:
APP (351)
Length:
3190
CDS:
151..2070

Additional Resources:

NCBI RefSeq record:
NM_001136129.3
NBCI Gene record:
APP (351)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001136129.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000011043 GCCATCTTTGACCGAAACGAA pLKO.1 1539 CDS 100% 3.000 4.200 N APP n/a
2 TRCN0000318477 GCCATCTTTGACCGAAACGAA pLKO_005 1539 CDS 100% 3.000 4.200 N APP n/a
3 TRCN0000006706 CGCTGCTTAGTTGGTGAGTTT pLKO.1 328 CDS 100% 4.950 3.960 N APP n/a
4 TRCN0000006705 CCCTGTTCATTGTAAGCACTT pLKO.1 2765 3UTR 100% 4.050 2.835 N APP n/a
5 TRCN0000349493 CCCTGTTCATTGTAAGCACTT pLKO_005 2765 3UTR 100% 4.050 2.835 N APP n/a
6 TRCN0000006707 GCAGACACAGACTATGCAGAT pLKO.1 619 CDS 100% 4.050 2.835 N APP n/a
7 TRCN0000318475 GCAGACACAGACTATGCAGAT pLKO_005 619 CDS 100% 4.050 2.835 N APP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001136129.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489725 CCATTTGTTTAATCGGTTTCATTC pLX_317 17.3% 91.9% 91.9% V5 (not translated due to prior stop codon) 57_58ins168 n/a
2 TRCN0000488121 TATATTTGTCGTTCATTTAGTTTT pLX_317 14.2% 91.8% 91.6% V5 (not translated due to prior stop codon) 57_58ins168;1617_1618delGAinsTC n/a
3 TRCN0000489208 GGCGGCCGGTGCCCTGCTTTGACC pLX_317 18.5% 91.7% 91.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10683 pDONR223 100% 35.2% 33.8% None (many diffs) n/a
5 ccsbBroad304_10683 pLX_304 0% 35.2% 33.8% V5 (many diffs) n/a
6 TRCN0000479912 GGTACCCAGATTCAGTATATAAGT pLX_317 43.5% 35.2% 33.8% V5 (many diffs) n/a
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