Construct: ORF TRCN0000479954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008890.1_s317c1
- Derived from:
- ccsbBroadEn_05973
- DNA Barcode:
- TGTATTTACTAAAAAATATTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC27 (996)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 996 | CDC27 | cell division cycle 27 | NM_001114091.4 | 99.9% | 99.8% | 1225A>G |
| 2 | human | 996 | CDC27 | cell division cycle 27 | XM_011525546.3 | 99.8% | 99.7% | 1225A>G;1394_1395insAGA |
| 3 | human | 996 | CDC27 | cell division cycle 27 | NM_001256.6 | 99.2% | 99.1% | 955_956insAGACTTTTCGTGTTTTAC;1207A>G |
| 4 | human | 996 | CDC27 | cell division cycle 27 | NM_001293089.3 | 99.1% | 99% | 955_956insAGACTTTTCGTGTTTTAC;1207A>G;1376_1377insAGA |
| 5 | human | 996 | CDC27 | cell division cycle 27 | XM_011525547.3 | 93.3% | 90.6% | 1225A>G;2251_2252ins64;2325_2326ins101 |
| 6 | human | 996 | CDC27 | cell division cycle 27 | XM_017025481.2 | 93.2% | 90.4% | (many diffs) |
| 7 | human | 996 | CDC27 | cell division cycle 27 | NM_001353035.2 | 92.6% | 89.8% | (many diffs) |
| 8 | human | 996 | CDC27 | cell division cycle 27 | XM_011525548.3 | 92.6% | 89.6% | 0_1ins35;68_69ins148;1042A>G |
| 9 | human | 996 | CDC27 | cell division cycle 27 | XM_011525549.2 | 92.6% | 89.6% | 0_1ins35;68_69ins148;1042A>G |
| 10 | human | 996 | CDC27 | cell division cycle 27 | NM_001353047.2 | 92.4% | 89.7% | (many diffs) |
| 11 | human | 996 | CDC27 | cell division cycle 27 | NM_001293091.3 | 91.8% | 88.9% | (many diffs) |
| 12 | human | 996 | CDC27 | cell division cycle 27 | XM_024451052.1 | 91.8% | 88.9% | (many diffs) |
| 13 | human | 996 | CDC27 | cell division cycle 27 | XM_017025484.2 | 91.7% | 88.7% | (many diffs) |
| 14 | human | 996 | CDC27 | cell division cycle 27 | NM_001353050.2 | 87.5% | 84.4% | 0_1ins35;67_68ins274;916A>G |
| 15 | human | 996 | CDC27 | cell division cycle 27 | XM_017025485.2 | 86.8% | 83.7% | (many diffs) |
| 16 | human | 996 | CDC27 | cell division cycle 27 | XM_017025486.1 | 86.7% | 83.6% | (many diffs) |
| 17 | human | 996 | CDC27 | cell division cycle 27 | NM_001353049.2 | 85.2% | 79.7% | (many diffs) |
| 18 | human | 102723570 | CDC27P9 | cell division cycle 27 pseu... | XR_430342.4 | 75.2% | (many diffs) | |
| 19 | human | 102724621 | LOC102724621 | cell division cycle protein... | XR_002958855.1 | 75.2% | (many diffs) | |
| 20 | human | 102723584 | CDC27P10 | cell division cycle 27 pseu... | XR_430343.4 | 74.3% | (many diffs) | |
| 21 | human | 102723603 | CDC27P11 | cell division cycle 27 pseu... | XR_430344.3 | 54.6% | (many diffs) | |
| 22 | human | 996 | CDC27 | cell division cycle 27 | XM_017025488.1 | 51.4% | 51.3% | 0_1ins1209;16A>G |
| 23 | human | 996 | CDC27 | cell division cycle 27 | NM_001353051.2 | 51.2% | 51.2% | 0_1ins1209;16A>G;185_186insAGA |
| 24 | human | 996 | CDC27 | cell division cycle 27 | XR_934630.2 | 44.3% | (many diffs) | |
| 25 | human | 996 | CDC27 | cell division cycle 27 | NR_148340.2 | 37.7% | (many diffs) | |
| 26 | mouse | 217232 | Cdc27 | cell division cycle 27 | XM_006533050.2 | 92.7% | 96.7% | (many diffs) |
| 27 | mouse | 217232 | Cdc27 | cell division cycle 27 | NM_001285988.1 | 92.6% | 96.6% | (many diffs) |
| 28 | mouse | 217232 | Cdc27 | cell division cycle 27 | NM_145436.2 | 92% | 96.1% | (many diffs) |
| 29 | mouse | 217232 | Cdc27 | cell division cycle 27 | NM_001285989.1 | 91.9% | 96% | (many diffs) |
| 30 | mouse | 217232 | Cdc27 | cell division cycle 27 | NM_001285990.1 | 77.2% | 78.7% | (many diffs) |
| 31 | mouse | 217232 | Cdc27 | cell division cycle 27 | XM_006533051.2 | 55.7% | 59% | (many diffs) |
| 32 | mouse | 217232 | Cdc27 | cell division cycle 27 | XM_006533052.3 | 55.7% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2556
- ORF length:
- 2490
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtgctgcag gaacccgtcc aggctgctat atggcaagca ctaaaccact 121 atgcttaccg agatgcggtt ttcctcgcag aacgccttta tgcagaagta cactcagaag 181 aagccttgtt tttactggca acctgttatt accgctcagg aaaggcatat aaagcatata 241 gactcttgaa aggacacagt tgtactacac cgcaatgcaa atacctgctt gcaaaatgtt 301 gtgttgatct cagcaagctt gcagaagggg aacaaatctt atctggtgga gtgtttaata 361 agcagaaaag ccatgatgat attgttactg agtttggtga ttcagcttgc tttactcttt 421 cattgttggg acatgtatat tgcaagacag atcggcttgc caaaggatca gaatgttacc 481 aaaagagcct tagtttaaat cctttcctct ggtctccctt tgaatcatta tgtgaaatag 541 gtgaaaagcc agatcctgac caaacattta aattcacatc tttacagaac tttagcaact 601 gtctgcccaa ctcttgcaca acacaagtac ctaatcatag tttatctcac agacagcctg 661 agacagttct tacggaaaca ccccaggaca caattgaatt aaacagattg aatttagaat 721 cttccaattc aaagtactcc ttgaatacag attcctcagt gtcttatatt gattcagctg 781 taatttcacc tgatactgtc ccactgggaa caggaacttc catattatct aaacaggttc 841 aaaataaacc aaaaactggt cgaagtttat taggaggacc agcagctctt agtccattaa 901 ccccaagttt tgggattttg ccattagaaa ccccaagtcc tggagatgga tcctatttac 961 aaaactacac taatacacct cctgtaattg atgtgccatc caccggagcc ccttcaaaaa 1021 agacttttcg tgttttacag tctgttgcca gaatcggcca aactggaaca aagtctgtct 1081 tctcacagag tggaaatagc cgagaggtaa ctccaattct tgcacaaaca caaagttctg 1141 gtccacaaac aagtacaaca cctcaggtat tgagccccac tattacatct cccccaaacg 1201 cactgcctcg aagaagttca cgactcttta ctagtgacag ctccacaacc aaggagaata 1261 gcaaaaaatt aaaaatgaag tttccacctg aaatcccaaa cagaaaaaca aaaagtaaaa 1321 ctaataaagg aggaataact caacctaaca taaatgatag cctggaaatt acaaaattgg 1381 actcttccat catttcagaa gggaaaatat ccacaatcac acctcagatt caggccttta 1441 atctacaaaa agcagcagca gaaggtttga tgagccttct tcgtgaaatg gggaaaggtt 1501 atttagcttt gtgttcatac aactgcaaag aagctataaa tattttgagc catctacctt 1561 ctcaccacta caatactggt tgggtactgt gccaaattgg aagggcctat tttgaacttt 1621 cagagtacat gcaagctgaa agaatattct cagaggttag aaggattgag aattatagag 1681 ttgaaggcat ggagatctac tctacaacac tttggcatct tcaaaaagat gttgctcttt 1741 cagttctgtc aaaagactta acagacatgg ataaaaattc gccagaggcc tggtgtgctg 1801 cagggaactg tttcagtctg caacgggaac atgatattgc aattaaattc ttccagagag 1861 ctatccaagt tgatccaaat tacgcttatg cctatactct attagggcat gagtttgtct 1921 taactgaaga attggacaaa gcattagctt gttttcgaaa tgctatcaga gtcaatccta 1981 gacattataa tgcatggtat ggtttaggaa tgatttatta caagcaagaa aaattcagcc 2041 ttgcagaaat gcatttccaa aaagcgcttg atatcaaccc tcaaagttca gttttacttt 2101 gccacattgg agtagttcaa catgcactga aaaaatcaga gaaggctttg gataccctaa 2161 acaaagccat tgtcattgat cccaagaacc ctctatgcaa atttcacaga gccTCAGTTT 2221 TATTTGCAAA TGAAAAATAT AAGTCTGCTT TACAAGAACT TGAAGAATTG AAACAAATTG 2281 TTCCCAAAGA ATCCCTCGTT TACTTCTTAA TAGGAAAGGT TTACAAGAAG TTAGGTCAAA 2341 CGCACCTCGC CCTGATGAAT TTCTCTTGGG CTATGGATTT AGATCCTAAA GGAGCCAATA 2401 ACCAGATTAA AGAGGCAATT GATAAGCGTT ATCTTCCAGA TGATGAGGAG CCAATAACCC 2461 AAGAAGAACA GATCATGGGA ACAGATGAAT CCCAGGAGAG CAGCATGACA GATGCGGATG 2521 ACACACAACT TCATGCAGCT GAAAGTGATG AATTTTACCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 ATGTATTTAC TAAAAAATAT TCCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt