Construct: ORF TRCN0000479998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016061.1_s317c1
- Derived from:
- ccsbBroadEn_08501
- DNA Barcode:
- ATACATACCTCCAAATAACTCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT15 (55270)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479998
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55270 | NUDT15 | nudix hydrolase 15 | NM_018283.4 | 99.7% | 100% | 384A>G |
| 2 | human | 55270 | NUDT15 | nudix hydrolase 15 | NM_001304745.1 | 79.9% | 65.7% | (many diffs) |
| 3 | human | 55270 | NUDT15 | nudix hydrolase 15 | NR_136688.1 | 16.8% | 1_180del;564A>G;673_2921del | |
| 4 | human | 55270 | NUDT15 | nudix hydrolase 15 | NR_136687.1 | 16.7% | 1_180del;564A>G;673_2928del | |
| 5 | mouse | 214254 | Nudt15 | nudix (nucleoside diphospha... | NM_172527.2 | 80.3% | 85.3% | (many diffs) |
| 6 | mouse | 214254 | Nudt15 | nudix (nucleoside diphospha... | XM_017315941.1 | 80.3% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 561
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggccagc gcacagccgc gcgggcggcg gccaggagtc ggagtcggag 121 tcgtggtgac cagctgcaag catccgcgtt gcgtcctcct ggggaagagg aaaggctcgg 181 ttggagctgg CAGTTTCCAA CTCCCTGGAG GTCATCTGGA GTTCGGTGAA ACCTGGGAAG 241 AATGTGCTCA AAGGGAAACC TGGGAAGAAG CAGCTCTTCA CCTGAAAAAT GTTCACTTTG 301 CCTCAGTTGT GAATTCTTTC ATTGAGAAGG AGAATTACCA TTATGTTACT ATATTAATGA 361 AAGGAGAAGT GGATGTGACT CATGATTCAG AACCAAAGAA TGTAGAGCCT GAAAAAAATG 421 AAAGTTGGGA GTGGGTTCCT TGGGAAGAAC TGCCTCCCCT GGACCAGCTT TTCTGGGGAC 481 TGCGTTGTTT AAAAGAACAA GGCTATGATC CATTTAAAGA AGATCTGAAC CATCTGGTGG 541 GATACAAAGG AAATCATCTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAC ATACCTCCAA 721 ATAACTCGAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt