Construct: ORF TRCN0000480000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014205.1_s317c1
- Derived from:
- ccsbBroadEn_15673
- DNA Barcode:
- TACACGGTCCCAAACAGCCATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE4DIP (9659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002810.3 | 92.9% | 91.8% | 74G>T;146T>C;519_519delGins37 |
| 2 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001195261.1 | 89.5% | 86.7% | (many diffs) |
| 3 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001195260.1 | 75.8% | 75.1% | 0_1ins96;50T>C;423_423delGins37 |
| 4 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_022359.6 | 51.3% | 50% | (many diffs) |
| 5 | human | 653513 | LOC653513 | phosphodiesterase 4D intera... | NR_037182.1 | 45.6% | (many diffs) | |
| 6 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002812.2 | 18.3% | 17.9% | (many diffs) |
| 7 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198832.2 | 7.7% | 7.3% | (many diffs) |
| 8 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002903.2 | 7.7% | 7.3% | (many diffs) |
| 9 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350523.1 | 7.6% | 7.3% | (many diffs) |
| 10 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510179.1 | 7.6% | 7.4% | (many diffs) |
| 11 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_014644.5 | 7.6% | 7.4% | (many diffs) |
| 12 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198834.3 | 7.5% | 7.3% | (many diffs) |
| 13 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002899.2 | 7.5% | 7.1% | (many diffs) |
| 14 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510176.2 | 7.4% | 7.1% | (many diffs) |
| 15 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451068.1 | 7.4% | 7% | (many diffs) |
| 16 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350522.1 | 7.3% | 7% | (many diffs) |
| 17 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711652.1 | 7.3% | 7.1% | (many diffs) |
| 18 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711651.1 | 7.3% | 7.1% | (many diffs) |
| 19 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711650.4 | 7.2% | 6.8% | (many diffs) |
| 20 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451067.1 | 7.1% | 6.8% | (many diffs) |
| 21 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350521.2 | 7% | 6.7% | (many diffs) |
| 22 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510175.2 | 6.9% | 6.6% | (many diffs) |
| 23 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002878.2 | 6.9% | 6.6% | (many diffs) |
| 24 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711646.2 | 6.9% | 6.6% | (many diffs) |
| 25 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510173.2 | 6.7% | 6.4% | (many diffs) |
| 26 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510172.2 | 6.7% | 6.4% | (many diffs) |
| 27 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002887.1 | 6% | 5.8% | (many diffs) |
| 28 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002888.1 | 6% | 5.8% | (many diffs) |
| 29 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002889.2 | 6% | 5.8% | (many diffs) |
| 30 | human | 100996724 | LOC100996724 | phosphodiesterase 4D intera... | NR_144517.1 | 4.9% | (many diffs) | |
| 31 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002891.2 | 4.8% | 4.2% | (many diffs) |
| 32 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002892.2 | 4.8% | 4.2% | (many diffs) |
| 33 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002893.1 | 4.8% | 4.2% | (many diffs) |
| 34 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002894.2 | 4.8% | 4.2% | (many diffs) |
| 35 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002895.2 | 4.8% | 4.2% | (many diffs) |
| 36 | human | 100996724 | LOC100996724 | phosphodiesterase 4D intera... | NR_144516.1 | 4.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc taatggatat cgcactctgt cccagcacct caatgacctg aagaaggaga 121 acttcagcct caagctgctc atctacttcc tggaggagcg catgcaacag aagtatgagg 181 ccagccgGGA GGACATCTAC AAGCGGAACA CTGAGCTGAA GGTTGAAGTG GAGAGCTTGA 241 AACGAGAACT CCAGGACAAG AAACAGCATC TGGATAAAAC ATGGGCTGAT GTGGAGAATC 301 TCAACAGTCA GAATGAAGCT GAGCTCCGAC GCCAGTTTGA GGAGCGACAG CAGGAGACGG 361 AGCATGTTTA TGAGCTCTTG GAGAATAAGA TCCAGCTTCT GCAGGAGGAA TCCAGGCTAG 421 CAAAGAATGA AGCTGCGCGG ATGGCAGCTC TGGTGGAAGC AGAGAAGGAG TGTAACCTGG 481 AGCTCTCAGA GAAACTGAAG GGAGTCACCA AAAACTGGGA AGATGTACCA GGAGACCAGG 541 TCAAGCCCGA CCAATACACT GAGGCCCTGG CCCAGAGGGA CAATCCTTCA ACTGACAGCT 601 TAAAGAACTT CAGGTTGTTC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA CGGTCCCAAA 781 CAGCCATTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt