Construct: ORF TRCN0000480011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001681.1_s317c1
- Derived from:
- ccsbBroadEn_08534
- DNA Barcode:
- CGACCGGTTCTTATGCTTCATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STRADB (55437)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55437 | STRADB | STE20 related adaptor beta | NM_018571.6 | 99.8% | 100% | 246A>G;609G>T |
| 2 | human | 55437 | STRADB | STE20 related adaptor beta | NM_001206864.2 | 89.5% | 88.9% | (many diffs) |
| 3 | human | 55437 | STRADB | STE20 related adaptor beta | XM_005246669.2 | 68% | 66.2% | (many diffs) |
| 4 | human | 55437 | STRADB | STE20 related adaptor beta | XM_017004438.1 | 66.9% | 66.9% | 0_1ins414;195G>T |
| 5 | mouse | 227154 | Stradb | STE20-related kinase adapto... | NM_172656.5 | 89.5% | 90.9% | (many diffs) |
| 6 | mouse | 227154 | Stradb | STE20-related kinase adapto... | XR_373251.2 | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcttttggat tgcttctgca cttcaagaac acaagttgaa tcactcagac 121 ctgaaaaaca gtctgaaacc agtatccatc aatacttggt tgatgagcca accctttcct 181 ggtcacgtcc atccactaga gccagtgaag tactatgttc caccaacgtt tctcactatg 241 agctccaagt agaaatagga agaggatttg acaacttgac ttctgtccat cttgcacggc 301 atactcccac gggaacactg gtaactataa aaattacaaa tctggaaaac tgcaatgaag 361 aacgcctgaa agctttacag aaagccgtga ttctatccca ctttttccgg catcccaata 421 ttacaactta ttggacagtt ttcactgttg gcagctggct ttgggttatt tctccattta 481 tggcctatgg ttcagcaagt caactcttga ggacctattt tcctgaagga atgagtgaaa 541 ctttaataag aaacattctc tttggagccg tgagagggtt gaactatctg caccaaaatg 601 gctgtattca caggagtatt aaagccagcc atatcctcat ttctggtgat ggcctagtga 661 ccctctctgg cctttcccat ctgcatagtt tggttaagca tggacagagg catagggctg 721 tgtatgattt cccacagttc agcacatcag tgcagccgtg gctgagtcca gaactactga 781 gacaggattt acatgggtat aatgtgaagt cagatattta cagtgttggg attacagcat 841 gtgaattagc cagtgggcag gtgcctttcc aggacatgca tagaactcag atgctgttac 901 agaaactgaa aggtcctccT TATAGCCCAT TGGATATCAG TATTTTCCCT CAATCAGAAT 961 CCAGAATGAA AAATTCCCAG TCAGGTGTAG ACTCTGGGAT TGGAGAAAGT GTGCTTGTCT 1021 CCAGTGGAAC TCACACAGTA AATAGTGACC GATTACACAC ACCATCCTCA AAAACTTTCT 1081 CTCCTGCCTT CTTTAGCTTG GTACAGCTCT GTTTGCAACA AGATCCTGAG AAAAGGCCAT 1141 CAGCAAGCAG TTTATTGTCC CATGTTTTCT TCAAACAGAT GAAAGAAGAA AGCCAGGATT 1201 CAATACTTTC ACTGTTGCCT CCTGCTTATA ACAAGCCATC AATATCATTG CCTCCAGTGT 1261 TACCTTGGAC TGAGCCAGAA TGTGATTTTC CTGATGAAAA AGACTCATAC TGGGAATTCT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACGACC GGTTCTTATG CTTCATGCAA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt